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The map label for this gene is aphA [H]

Identifier: 148825228

GI number: 148825228

Start: 103118

End: 103825

Strand: Direct

Name: aphA [H]

Synonym: CGSHiEE_00520

Alternate gene names: 148825228

Gene position: 103118-103825 (Clockwise)

Preceding gene: 148825225

Following gene: 148825233

Centisome position: 5.69

GC content: 38.14

Gene sequence:

>708_bases
ATGAAAAACGTCATGAAATTATCTGTAATCGCACTTTTAACTGCAGCTGCAGTTCCTGCGATGGCAGGCAAAACAGAACC
TTATACTCAATCTGGCACTAATGCTCGTGAAATGTTACAAGAGCAAGCAATTCATTGGATTAGTGTTGATCAAATCAAAC
AAAGTTTAGAAGGTAAAGCACCAATTAATGTAAGCTTTGATATTGATGATACCGTGCTTTTCAGTAGCCCTTGCTTTTAC
CACGGACAACAAAAATTCTCACCAGGTAAACACGATTATTTGAAAAACCAAGATTTTTGGAATGAAGTAAATGCAGGTTG
CGATAAATATTCTATTCCTAAACAAATTGCCATAGATTTAATTAATATGCACCAAGCACGTGGCGACCAAGTTTACTTTT
TCACTGGCCGTACTGCTGGCAAAGTGGATGGTGTAACACCAATTTTAGAAAAAACGTTCAATATTAAGAATATGCACCCA
GTTGAATTTATGGGCAGCCGTGAACGTACAACAAAATATAACAAAACGCCTGCAATCATTTCACATAAAGTGAGTATTCA
CTATGGCGATAGCGATGATGATGTGCTAGCAGCTAAAGAAGCGGGTGTTCGTGGTATTCGTTTAATGCGTGCCGCAAACT
CTACTTATCAACCAATGCCAACTTTAGGCGGCTATGGCGAAGAAGTGTTAATCAATTCAAGCTATTAA

Upstream 100 bases:

>100_bases
ATCATACTTTGAAACAGCAAACGATTGCTAATTATTGAATATAAGCTAAAAACATGTATGATGTGAGCGGTTTTTAAATC
AACGTTTGTGAGGAAAGGCA

Downstream 100 bases:

>100_bases
TTGACCGCAAGTTGGGATTTATCTTCCCCCAAAAAGTTAGATTGATTTAGTTCAAGGACTGAGTTCTGTCATTCACAGGA
CTCAGCCCTTTTAGTTTCCC

Product: acid phosphatase/phosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKNVMKLSVIALLTAAAVPAMAGKTEPYTQSGTNAREMLQEQAIHWISVDQIKQSLEGKAPINVSFDIDDTVLFSSPCFY
HGQQKFSPGKHDYLKNQDFWNEVNAGCDKYSIPKQIAIDLINMHQARGDQVYFFTGRTAGKVDGVTPILEKTFNIKNMHP
VEFMGSRERTTKYNKTPAIISHKVSIHYGDSDDDVLAAKEAGVRGIRLMRAANSTYQPMPTLGGYGEEVLINSSY

Sequences:

>Translated_235_residues
MKNVMKLSVIALLTAAAVPAMAGKTEPYTQSGTNAREMLQEQAIHWISVDQIKQSLEGKAPINVSFDIDDTVLFSSPCFY
HGQQKFSPGKHDYLKNQDFWNEVNAGCDKYSIPKQIAIDLINMHQARGDQVYFFTGRTAGKVDGVTPILEKTFNIKNMHP
VEFMGSRERTTKYNKTPAIISHKVSIHYGDSDDDVLAAKEAGVRGIRLMRAANSTYQPMPTLGGYGEEVLINSSY
>Mature_235_residues
MKNVMKLSVIALLTAAAVPAMAGKTEPYTQSGTNAREMLQEQAIHWISVDQIKQSLEGKAPINVSFDIDDTVLFSSPCFY
HGQQKFSPGKHDYLKNQDFWNEVNAGCDKYSIPKQIAIDLINMHQARGDQVYFFTGRTAGKVDGVTPILEKTFNIKNMHP
VEFMGSRERTTKYNKTPAIISHKVSIHYGDSDDDVLAAKEAGVRGIRLMRAANSTYQPMPTLGGYGEEVLINSSY

Specific function: Dephosphorylates Several Organic Phosphomonoesters And Catalyzes The Transfer Of Low-Energy Phosphate Groups From Phosphomonoesters To Hydroxyl Groups Of Various Organic Compounds. Preferentially Acts On Aryl Phosphoesters. Maximal Activity At Ph 6-6.5 To

COG id: COG3700

COG function: function code R; Acid phosphatase (class B)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class B bacterial acid phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI2367341, Length=232, Percent_Identity=46.1206896551724, Blast_Score=221, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005519
- InterPro:   IPR023214
- InterPro:   IPR010025 [H]

Pfam domain/function: PF03767 Acid_phosphat_B [H]

EC number: =3.1.3.2 [H]

Molecular weight: Translated: 26140; Mature: 26140

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVMKLSVIALLTAAAVPAMAGKTEPYTQSGTNAREMLQEQAIHWISVDQIKQSLEGKA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEECHHHHHHHHCCCC
PINVSFDIDDTVLFSSPCFYHGQQKFSPGKHDYLKNQDFWNEVNAGCDKYSIPKQIAIDL
CEEEEECCCCEEEECCCCEECCCCCCCCCCHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
INMHQARGDQVYFFTGRTAGKVDGVTPILEKTFNIKNMHPVEFMGSRERTTKYNKTPAII
HHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCEE
SHKVSIHYGDSDDDVLAAKEAGVRGIRLMRAANSTYQPMPTLGGYGEEVLINSSY
EEEEEEEECCCCCCEEEHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MKNVMKLSVIALLTAAAVPAMAGKTEPYTQSGTNAREMLQEQAIHWISVDQIKQSLEGKA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEECHHHHHHHHCCCC
PINVSFDIDDTVLFSSPCFYHGQQKFSPGKHDYLKNQDFWNEVNAGCDKYSIPKQIAIDL
CEEEEECCCCEEEECCCCEECCCCCCCCCCHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
INMHQARGDQVYFFTGRTAGKVDGVTPILEKTFNIKNMHPVEFMGSRERTTKYNKTPAII
HHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCEE
SHKVSIHYGDSDDDVLAAKEAGVRGIRLMRAANSTYQPMPTLGGYGEEVLINSSY
EEEEEEEECCCCCCEEEHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]