Click here to switch to the map view.

The map label for this gene is priB

Identifier: 148825180

GI number: 148825180

Start: 48576

End: 48902

Strand: Direct

Name: priB

Synonym: CGSHiEE_00260

Alternate gene names: 148825180

Gene position: 48576-48902 (Clockwise)

Preceding gene: 148825179

Following gene: 148825181

Centisome position: 2.68

GC content: 35.17

Gene sequence:

>327_bases
ATGCTGAAGAGTAATTTGAAAATTGATAATCGTTTTTCGGTTATGGGAGTCGTTTCTCAGTTACCAAAGCGATTAAAAAG
CCCTAGTGGAATTGAGCATTGTAAGTTTTTGTTAGAACATCGTTCTGATCAGATTGAAAGTGGTTTCACTCGTCAAGCGT
GGTTAAAAATGCCAGTTCAAATTAGTGGTAATCAATTGATAGAAAAAACTCAAAGCATTACGGTCGGTAGTAAAATTTTG
GTGGTGGGATTTATTACATCACATAAAACCCAAAGTGGGTTATGCCAATTAGTTTTACATGCCGAGCAAATCGAATTTAT
AGATTAG

Upstream 100 bases:

>100_bases
GTTATCCATACTAAGCACGCCGTAACAGAAGCGTCCCCAATGAAAGCGGCTAAAGAAGAACGTAAACCTTTAGCTGAAGT
TGAAAACAACGATTTTGAGG

Downstream 100 bases:

>100_bases
GAGACTAGCCAAATGGCACGTTATTTCCGTCGTCGTAAGTTCTGCCGTTTCACAGCGGAAAATGTTGTTGAAATCGATTA
CAAAGATATCGCTACATTAA

Product: primosomal replication protein N

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 108

Protein sequence:

>108_residues
MLKSNLKIDNRFSVMGVVSQLPKRLKSPSGIEHCKFLLEHRSDQIESGFTRQAWLKMPVQISGNQLIEKTQSITVGSKIL
VVGFITSHKTQSGLCQLVLHAEQIEFID

Sequences:

>Translated_108_residues
MLKSNLKIDNRFSVMGVVSQLPKRLKSPSGIEHCKFLLEHRSDQIESGFTRQAWLKMPVQISGNQLIEKTQSITVGSKIL
VVGFITSHKTQSGLCQLVLHAEQIEFID
>Mature_108_residues
MLKSNLKIDNRFSVMGVVSQLPKRLKSPSGIEHCKFLLEHRSDQIESGFTRQAWLKMPVQISGNQLIEKTQSITVGSKIL
VVGFITSHKTQSGLCQLVLHAEQIEFID

Specific function: Binds single-stranded DNA at the primosome assembly site (PAS)

COG id: COG2965

COG function: function code L; Primosomal replication protein N

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain

Homologues:

Organism=Escherichia coli, GI1790645, Length=101, Percent_Identity=57.4257425742574, Blast_Score=119, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIB_HAEI8 (Q4QN02)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248255.1
- ProteinModelPortal:   Q4QN02
- SMR:   Q4QN02
- STRING:   Q4QN02
- GeneID:   3429982
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0672
- eggNOG:   COG2965
- HOGENOM:   HBG632331
- OMA:   WCRMPVI
- PhylomeDB:   Q4QN02
- ProtClustDB:   PRK02801
- BioCyc:   HINF281310:NTHI0672-MONOMER
- HAMAP:   MF_00720
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF00436 SSB; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 12148; Mature: 12148

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSNLKIDNRFSVMGVVSQLPKRLKSPSGIEHCKFLLEHRSDQIESGFTRQAWLKMPVQ
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCCCCCHHHHCCCEE
ISGNQLIEKTQSITVGSKILVVGFITSHKTQSGLCQLVLHAEQIEFID
ECCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKSNLKIDNRFSVMGVVSQLPKRLKSPSGIEHCKFLLEHRSDQIESGFTRQAWLKMPVQ
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHCCCCCHHHHCCCEE
ISGNQLIEKTQSITVGSKILVVGFITSHKTQSGLCQLVLHAEQIEFID
ECCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA