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The map label for this gene is limB [H]

Identifier: 148824822

GI number: 148824822

Start: 4071701

End: 4072888

Strand: Direct

Name: limB [H]

Synonym: TBFG_13649

Alternate gene names: 148824822

Gene position: 4071701-4072888 (Clockwise)

Preceding gene: 148824821

Following gene: 148824827

Centisome position: 92.03

GC content: 64.06

Gene sequence:

>1188_bases
ATGAAGGCACCGTTGCGTTTTGGCGTTTTCATCACGCCATTCCATCCGACCGGTCAATCCCCGACCGTGGCGTTGCAATA
CGACATGGAGCGCGTCGTTGCGCTGGACCGGCTCGGCTACGACGAGGCGTGGTTTGGCGAACACCACTCCGGTGGCTACG
AGCTGATCGCTTGCCCGGAGGTGTTTATCGCGGCCGCAGCGGAACGGACCACCCACATCCGGCTAGGTACCGGAGTGGTT
TCGCTGCCCTACCATCATCCGCTAATGGTGGCCGACCGTTGGGTGCTGCTGGATCACCTGACCCGTGGGCGGGTCATGTT
CGGCACCGGCCCCGGCGCGCTGCCGTCGGACGCCTACATGATGGGCATCGATCCGGTCGAGCAGCGACGAATGATGCAGG
AGTCCCTCGAGGCGATTCTCGCGCTGTTCCGTGCCGCACCTGACGAGCGAATCGACCGCCACTCCGACTGGTTCACCCTG
CGTGAAGCGCAATTGCACATCCGCCCCTACACCTGGCCGTACCCCGAAATCGCTACCGCAGCCATGATTTCGCCATCGGG
TCCGCGACTGGCCGGTGCGCTGGGCACGTCGCTGTTATCACTGTCGATGTCAGTGCCCGGCGGCTACGCTGCGCTGGAAA
CAGCGTGGGGCGTGGTGCGGGAGCAGGCCGCCAAAGCTGGGCGGGGCGAGCCGGATCGCGCCGATTGGCGGGTGTTGAGC
ATCATGCACTTGTCGGACAGCCGCGACCAGGCGATCGACGACTGCACTTACGGGTTACCCGACTTCTCGAGGTACTTCGG
CGCGGCAGGGTTTGTCCCGTTGGCGAACACCGTGGAAGGCACCCAGTCGTCTCGGGAATTCGTCGAGCAATACGCGGCCA
AGGGAAATTGCTGCATCGGCACGCCCGATGACGCGATCGCCCACATTGAAGACTTGCTGCACCGGTCGGGTGGCTTCGGA
ACGTTGCTACTGCTCGGCCACGACTGGGCCCCGCCACCGGCAACCTTTCACTCCTATGAGCTGTTCGCCCGTGCTGTGAT
TCCTTATTTCAAGGGACAACTCGCGGCGCCGCGGGCGTCGCACGAATGGGCTAGAGGCAAGCGCGACCAATTGATTGGCC
GCGCCGGCGAAGCGGTCGTCAAAGCCATCACCGAGCACGTCGCCGAACAAGGGGAAGCGGGCAGCTGA

Upstream 100 bases:

>100_bases
GCGAGGTGCTGATCGACGGGGCCGGTCACTGGCTGCAGCAGGAACGTCCCGGTGAGGTGACCGCGGCCCTGCTGGAGTTC
CTGACGGGGTTGGAGTTGCG

Downstream 100 bases:

>100_bases
CGCGGGCGCAGTGTTCCCGACGACGACATGCCCGTGTATCGGGCGCCAAAGTCGACGCTGATCGGCCCGCCCTGCGCGGA
CCCAACTTAGGACCCGGGTT

Product: monooxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPEVFIAAAAERTTHIRLGTGVV
SLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTL
REAQLHIRPYTWPYPEIATAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLS
IMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIGTPDDAIAHIEDLLHRSGGFG
TLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRASHEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS

Sequences:

>Translated_395_residues
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPEVFIAAAAERTTHIRLGTGVV
SLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTL
REAQLHIRPYTWPYPEIATAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLS
IMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIGTPDDAIAHIEDLLHRSGGFG
TLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRASHEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS
>Mature_395_residues
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPEVFIAAAAERTTHIRLGTGVV
SLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYMMGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTL
REAQLHIRPYTWPYPEIATAAMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLS
IMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIGTPDDAIAHIEDLLHRSGGFG
TLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRASHEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS

Specific function: Acts on both enantiomers of limonene by their NAD- dependent epoxidation at the 1,2 double bond forming limonene-1,2- epoxide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial luciferase oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1789551, Length=154, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.14.13.107 [H]

Molecular weight: Translated: 43278; Mature: 43278

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPE
CCCCEEECEEEECCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHH
VFIAAAAERTTHIRLGTGVVSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYM
HHHHHHHCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEE
MGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTLREAQLHIRPYTWPYPEIATA
ECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHH
AMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLS
EEECCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
IMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIG
EEECCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEC
TPDDAIAHIEDLLHRSGGFGTLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRAS
CCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
HEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKAPLRFGVFITPFHPTGQSPTVALQYDMERVVALDRLGYDEAWFGEHHSGGYELIACPE
CCCCEEECEEEECCCCCCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHH
VFIAAAAERTTHIRLGTGVVSLPYHHPLMVADRWVLLDHLTRGRVMFGTGPGALPSDAYM
HHHHHHHCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEE
MGIDPVEQRRMMQESLEAILALFRAAPDERIDRHSDWFTLREAQLHIRPYTWPYPEIATA
ECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHH
AMISPSGPRLAGALGTSLLSLSMSVPGGYAALETAWGVVREQAAKAGRGEPDRADWRVLS
EEECCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
IMHLSDSRDQAIDDCTYGLPDFSRYFGAAGFVPLANTVEGTQSSREFVEQYAAKGNCCIG
EEECCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEC
TPDDAIAHIEDLLHRSGGFGTLLLLGHDWAPPPATFHSYELFARAVIPYFKGQLAAPRAS
CCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
HEWARGKRDQLIGRAGEAVVKAITEHVAEQGEAGS
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA