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The map label for this gene is truA

Identifier: 148824665

GI number: 148824665

Start: 3889763

End: 3890656

Strand: Reverse

Name: truA

Synonym: TBFG_13492

Alternate gene names: 148824665

Gene position: 3890656-3889763 (Counterclockwise)

Preceding gene: 148824666

Following gene: 148824660

Centisome position: 87.94

GC content: 67.79

Gene sequence:

>894_bases
GTGAGCCTGACGCGAAGGCCCCCGAAGAGCCCCCCGCAGAGGCCCCCGAGAATTAGCGGCGTTGTCCGTCTTCGGCTCGA
TATCGCCTACGACGGAACCGATTTCGCGGGCTGGGCTGCACAAGTGGGGCAGCGCACGGTCGCGGGCGATCTCGATGCGG
CGCTGACTACCATCTTCCGCACCCCGGTGCGGCTGCGGGCGGCCGGACGCACTGATGCGGGCGTGCACGCCAGCGGCCAG
GTCGCCCACGTTGACGTGCCCGCCGATGCCTTGCCGAACGCTTACCCGCGCGCCGGCCACGTCGGTGATCCGGAATTCCT
GCCGCTGTTGCGGAGGCTGGGTCGATTTCTGCCCGCCGACGTTCGAATCCTCGATATCACGCGCGCACCAGCGGGTTTCG
ACGCCAGATTCTCGGCGCTGCGGCGGCATTACGTGTACCGGCTGTCGACAGCACCCTACGGTGTCGAGCCGCAGCAGGCG
CGCTACATCACCGCTTGGCCACGCGAGCTTGATCTGGATGCGATGACGGCCGCGTCGCGAGATTTGATGGGGCTGCACGA
CTTCGCGGCGTTCTGCCGTCATCGCGAGGGCGCCACCACGATTCGTGATCTGCAGCGGCTGGACTGGTCGCGTGCCGGCA
CCCTGGTCACCGCGCACGTCACCGCCGACGCGTTCTGTTGGTCGATGGTGCGGTCGTTGGTGGGGGCGCTACTGGCGGTC
GGCGAGCACCGTCGCGCGACAACTTGGTGTCGTGAGCTGCTCACCGCGACAGGACGATCCAGCGACTTCGCGGTCGCGCC
GGCGCACGGGTTGACGTTGATCCAGGTGGACTATCCGCCCGATGACCAGCTCGCGTCACGGAACTTGGTCACCCGGGATG
TGCGCTCGGGCTAA

Upstream 100 bases:

>100_bases
CGGGTAGCTGCCGCGCAGGCGAAGGCCAAGAAGGCGGCGGCCATGCCGACCGAGGAGTCCGAGGCCAAGCCTGCTGAGGA
GGGTGACGTGGTCGGCGCCA

Downstream 100 bases:

>100_bases
CCCAGGAAGCACATGCTCGGCCTGCTTGTCCGTGGACAAGCACAGCGAACAGACATGCGCACCATGGGTCTGGCAGGCCA
GCATGTCGGGCCGCTCGAAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSLTRRPPKSPPQRPPRISGVVRLRLDIAYDGTDFAGWAAQVGQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQ
VAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPADVRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQA
RYITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAV
GEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPDDQLASRNLVTRDVRSG

Sequences:

>Translated_297_residues
MSLTRRPPKSPPQRPPRISGVVRLRLDIAYDGTDFAGWAAQVGQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQ
VAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPADVRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQA
RYITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAV
GEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPDDQLASRNLVTRDVRSG
>Mature_296_residues
SLTRRPPKSPPQRPPRISGVVRLRLDIAYDGTDFAGWAAQVGQRTVAGDLDAALTTIFRTPVRLRAAGRTDAGVHASGQV
AHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPADVRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQAR
YITAWPRELDLDAMTAASRDLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAVG
EHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPDDQLASRNLVTRDVRSG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=293, Percent_Identity=33.7883959044369, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI199559785, Length=262, Percent_Identity=27.8625954198473, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788657, Length=275, Percent_Identity=33.8181818181818, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321112, Length=257, Percent_Identity=27.2373540856031, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_MYCBO (P65847)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857124.2
- ProteinModelPortal:   P65847
- SMR:   P65847
- EnsemblBacteria:   EBMYCT00000016541
- GeneID:   1091090
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3484c
- GeneTree:   EBGT00050000017011
- HOGENOM:   HBG621020
- ProtClustDB:   PRK00021
- BioCyc:   MBOV233413:MB3484C-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 32620; Mature: 32489

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: ACT_SITE 72-72

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRRPPKSPPQRPPRISGVVRLRLDIAYDGTDFAGWAAQVGQRTVAGDLDAALTTIFR
CCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHCCCHHHCHHHHHHHHHHH
TPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPAD
CCHHEEECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
VRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQARYITAWPRELDLDAMTAASR
EEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCHHHCEEEEECCCCCCHHHHHHHHH
DLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
GEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPDDQLASRNLVTRDVRSG
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
SLTRRPPKSPPQRPPRISGVVRLRLDIAYDGTDFAGWAAQVGQRTVAGDLDAALTTIFR
CCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHCCCHHHCHHHHHHHHHHH
TPVRLRAAGRTDAGVHASGQVAHVDVPADALPNAYPRAGHVGDPEFLPLLRRLGRFLPAD
CCHHEEECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
VRILDITRAPAGFDARFSALRRHYVYRLSTAPYGVEPQQARYITAWPRELDLDAMTAASR
EEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCHHHCEEEEECCCCCCHHHHHHHHH
DLMGLHDFAAFCRHREGATTIRDLQRLDWSRAGTLVTAHVTADAFCWSMVRSLVGALLAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
GEHRRATTWCRELLTATGRSSDFAVAPAHGLTLIQVDYPPDDQLASRNLVTRDVRSG
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972