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The map label for this gene is fmt [C]

Identifier: 148824612

GI number: 148824612

Start: 3835172

End: 3835876

Strand: Reverse

Name: fmt [C]

Synonym: TBFG_13439

Alternate gene names: 148824612

Gene position: 3835876-3835172 (Counterclockwise)

Preceding gene: 148824613

Following gene: 148824611

Centisome position: 86.7

GC content: 59.15

Gene sequence:

>705_bases
GTGACGATATTGATCCTGACCGACAACGTCCACGCCCATGCTCTGGCGGTCGATCTGCAGGCCAGGCATGGCGATATGGA
CGTCTATCAGTCCCCCATCGGCCAGCTGCCGGGTGTCCCGCGATGTGATGTCGCAGAGCGCGTCGCGGAAATCGTGGAGC
GGTATGACCTCGTCCTTTCCTTCCACTGTAAACAGAGGTTTCCCGCCGCTTTGATCGATGGGGTCAGGTGTGTGAATGTT
CATCCGGGTTTCAACCCCTACAACCGCGGCTGGTTTCCCCAGGTCTTCTCGATCATCGACGGGCAAAAAGTCGGCGTGAC
GATCCACGAGATCGACGATCAGTTGGACCATGGTCCGATCATCGCCCAGCGGGAATGCGCGATCGAGTCGTGGGATTCCT
CGGGAAGTGTCTACGCCCGGCTGATGGACATCGAGCGTGAGTTGGTGCTGGAACATTTCGACGCCATCCGGGACGGCAGC
TACACGGCTAAATCGCCGGCCACCGAGGGCAACCTCAACCTGAAAAAGGATTTCGAACAACTCCGGCGGCTAGACCTGAA
CGAGCGCGGAACGTTTGGGCATTTCCTGAATCGCCTGCGCGCGTTGACCCATGATGATTTCCGCAACGCTTGGTTCGTCG
ATGCGTCAGGCCGCAAGGTGTTTGTCCGCGTCGTGCTCGAACCGGAGAAGCCCGCGGAAGCCTAA

Upstream 100 bases:

>100_bases
GCCCCAGCGTGGCCGTCCGGTATGCGTCGGCCCTGAAGTAGGGTGAGCTGGTGTTGGGGGCTCCGCTGATGGGCGCTGGG
CAGGCTGGCGGGGGCGAGTC

Downstream 100 bases:

>100_bases
CCACCGCCTCGAGGTTATGTTAGCCTTCCCTTATTTGATGACTATGATCACTCCACCTACCTTCGACGTTGCGTTCATCG
GCAGCGGGGCCGCGTGCTCT

Product: hypothetical protein

Products: tetrahydrofolate; N-formylmethionyl-tRNA [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTILILTDNVHAHALAVDLQARHGDMDVYQSPIGQLPGVPRCDVAERVAEIVERYDLVLSFHCKQRFPAALIDGVRCVNV
HPGFNPYNRGWFPQVFSIIDGQKVGVTIHEIDDQLDHGPIIAQRECAIESWDSSGSVYARLMDIERELVLEHFDAIRDGS
YTAKSPATEGNLNLKKDFEQLRRLDLNERGTFGHFLNRLRALTHDDFRNAWFVDASGRKVFVRVVLEPEKPAEA

Sequences:

>Translated_234_residues
MTILILTDNVHAHALAVDLQARHGDMDVYQSPIGQLPGVPRCDVAERVAEIVERYDLVLSFHCKQRFPAALIDGVRCVNV
HPGFNPYNRGWFPQVFSIIDGQKVGVTIHEIDDQLDHGPIIAQRECAIESWDSSGSVYARLMDIERELVLEHFDAIRDGS
YTAKSPATEGNLNLKKDFEQLRRLDLNERGTFGHFLNRLRALTHDDFRNAWFVDASGRKVFVRVVLEPEKPAEA
>Mature_233_residues
TILILTDNVHAHALAVDLQARHGDMDVYQSPIGQLPGVPRCDVAERVAEIVERYDLVLSFHCKQRFPAALIDGVRCVNVH
PGFNPYNRGWFPQVFSIIDGQKVGVTIHEIDDQLDHGPIIAQRECAIESWDSSGSVYARLMDIERELVLEHFDAIRDGSY
TAKSPATEGNLNLKKDFEQLRRLDLNERGTFGHFLNRLRALTHDDFRNAWFVDASGRKVFVRVVLEPEKPAEA

Specific function: Modifies The Free Amino Group Of The Aminoacyl Moiety Of Methionyl-Trna(Fmet). The Formyl Group Appears To Play A Dual Role In The Initiator Identity Of N-Formylmethionyl-Trna By: (I) Promoting Its Recognition By If2 And (II) Impairing Its Binding To Eftu

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): Y3404_MYCTU (P65073)

Other databases:

- EMBL:   BX842583
- EMBL:   AE000516
- PIR:   A70736
- RefSeq:   NP_217921.1
- RefSeq:   NP_338036.1
- ProteinModelPortal:   P65073
- EnsemblBacteria:   EBMYCT00000001572
- EnsemblBacteria:   EBMYCT00000073071
- GeneID:   887902
- GeneID:   926605
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3512
- KEGG:   mtu:Rv3404c
- TIGR:   MT3512
- TubercuList:   Rv3404c
- GeneTree:   EBGT00050000018465
- HOGENOM:   HBG565354
- OMA:   GATIHEM
- ProtClustDB:   PRK07579
- GO:   GO:0009405
- InterPro:   IPR002376
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138

Pfam domain/function: PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: 2.1.2.9 [C]

Molecular weight: Translated: 26515; Mature: 26384

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILILTDNVHAHALAVDLQARHGDMDVYQSPIGQLPGVPRCDVAERVAEIVERYDLVLS
CEEEEEECCCCEEEEEEEEECCCCCHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHE
FHCKQRFPAALIDGVRCVNVHPGFNPYNRGWFPQVFSIIDGQKVGVTIHEIDDQLDHGPI
EHHHHCCCHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCCCC
IAQRECAIESWDSSGSVYARLMDIERELVLEHFDAIRDGSYTAKSPATEGNLNLKKDFEQ
EECHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
LRRLDLNERGTFGHFLNRLRALTHDDFRNAWFVDASGRKVFVRVVLEPEKPAEA
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
TILILTDNVHAHALAVDLQARHGDMDVYQSPIGQLPGVPRCDVAERVAEIVERYDLVLS
EEEEEECCCCEEEEEEEEECCCCCHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHE
FHCKQRFPAALIDGVRCVNVHPGFNPYNRGWFPQVFSIIDGQKVGVTIHEIDDQLDHGPI
EHHHHCCCHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEEHHHHCCCCCCCCC
IAQRECAIESWDSSGSVYARLMDIERELVLEHFDAIRDGSYTAKSPATEGNLNLKKDFEQ
EECHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
LRRLDLNERGTFGHFLNRLRALTHDDFRNAWFVDASGRKVFVRVVLEPEKPAEA
HHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; K+; Mg2+; Na+; NH4+ [C]

Kcat value (1/min): 1200 [C]

Specific activity: 1.14

Km value (mM): 0.0006 {methionyl-tRNAfMet}} 0.00035 {methionyl-tRNAfMet}} 0.013 {10-formyltetrahydrofolate}} 0.0013 {phenylalanyl-tRNAfMet}} 0.0014 {valyl-tRNAfMet}} [C]

Substrates: Formyltetrahydrofolate; L-methionyl-tRNA; H2O [C]

Specific reaction: Formyltetrahydrofolate + L-methionyl-tRNA + H2O =tetrahydrofolate + N-formylmethionyl-tRNA [C]

General reaction: Formyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036