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The map label for this gene is 148824580

Identifier: 148824580

GI number: 148824580

Start: 3797407

End: 3798747

Strand: Direct

Name: 148824580

Synonym: TBFG_13407

Alternate gene names: NA

Gene position: 3797407-3798747 (Clockwise)

Preceding gene: 148824577

Following gene: 148824581

Centisome position: 85.83

GC content: 65.92

Gene sequence:

>1341_bases
ATGGCACAACTGACGGCACTGGATGCGGGTTTTCTCAAGTCCCGCGATCCGGAGCGGCACCCGGGCCTGGCGATCGGCGC
AGTTGCCGTCGTCAACGGTGCCGCCCCCAGCTACGACCAGCTCAAAACGGTTCTCACAGAACGGATTAAGTCGATACCTC
GATGTACCCAGGTGTTGGCGACCGAGTGGATCGACTATCCGGGATTCGACCTCACCCAGCACGTGCGACGGGTGGCGCTT
CCCCGGCCCGGCGACGAAGCCGAGCTGTTCCGGGCCATCGCGCTGGCACTGGAGCGTCCCCTCGACCCGGACCGCCCGCT
GTGGGAATGCTGGATCATCGAAGGCCTCAACGGCAACCGCTGGGCGATCTTGATAAAAATCCACCATTGCATGGCCGGCG
CCATGTCGGCGGCCCACCTGCTGGCCAGGCTCTGCGACGATGCCGACGGCAGTGCCTTCGCTAACAATGTTGATATCAAA
CAGATTCCGCCGTATGGCGATGCGCGGAGCTGGGCCGAAACGCTGTGGCGAATGTCCGTCAGCATCGCTGGCGCCGTCTG
CACGGCCGCGGCACGCGCCGTCAGCTGGCCGGCAGTGACGTCACCGGCCGGCCCGGTCACCACCAGGCGGCGGTACCAAG
CGGTGCGCGTTCCCCGCGACGCCGTCGACGCCGTGTGCCACAAGTTCGGGGTGACCGCCAACGACGTCGCGCTCGCGGCC
ATCACCGAGGGCTTCCGAACGGTTCTGCTGCACCGCGGCCAGCAACCGCGCGCCGACTCACTGCGTACCCTGGAGAAAAC
CGATGGCAGCTCGGCCATGCTGCCCTATCTCCCCGTCGAGTACGACGACCCGGTGCGGCGATTGCGCACCGTGCACAACC
GGTCACAGCAGAGCGGCCGTCGTCAACCCGACAGTCTGTCGGACTATACGCCTCTCATGTTGTGCGCCAAGATGATTCAC
GCGCTAGCTCGGTTACCGCAACAAGGCATCGTCACCCTGGCGACCAGTGCACCCAGGCCACGCCACCAGTTACGGCTGAT
GGGCCAGAAGATGGACCAGGTGCTGCCCATCCCGCCCACCGCACTGCAGCTGAGCACCGGGATCGCGGTCCTCAGCTACG
GCGATGAGCTGGTGTTCGGCATCACCGCTGACTATGACGCCGCGTCCGAAATGCAGCAGCTGGTCAACGGTATCGAACTG
GGTGTGGCGCGTCTGGTGGCGCTCAGCGACGATTCCGTGCTGCTGTTTACCAAGGATCGGCGTAAGCGTTCATCCCGCGC
ACTCCCCAGCGCCGCGCGGCGGGGGCGGCCCTCTGTGCCGACCGCCCGAGCGCGTCACTGA

Upstream 100 bases:

>100_bases
CACCTGCGAAACATGTTCTTAACCGTTTGGGCCTTCTGCTTCCGGTGCGGTCCGGCGGACACTTATACCTGGGGTCGCAA
AACGACGGTGGGGACTTGTC

Downstream 100 bases:

>100_bases
CGCCATCTCCGTCGGCGTTGACCCCCGTGAGAGGGTGGGTCGTGCGCAAGTTGGGCCCGGTCACCATCGATCCGCGCCGC
CATGACGCGGTGCTGTTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3371/MT3481

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVAL
PRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIK
QIPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIH
ALARLPQQGIVTLATSAPRPRHQLRLMGQKMDQVLPIPPTALQLSTGIAVLSYGDELVFGITADYDAASEMQQLVNGIEL
GVARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH

Sequences:

>Translated_446_residues
MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVAL
PRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIK
QIPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIH
ALARLPQQGIVTLATSAPRPRHQLRLMGQKMDQVLPIPPTALQLSTGIAVLSYGDELVFGITADYDAASEMQQLVNGIEL
GVARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH
>Mature_445_residues
AQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVALP
RPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQ
IPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAAI
TEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIHA
LARLPQQGIVTLATSAPRPRHQLRLMGQKMDQVLPIPPTALQLSTGIAVLSYGDELVFGITADYDAASEMQQLVNGIELG
VARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y3371_MYCTU (O50400)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   B70972
- RefSeq:   NP_217888.1
- RefSeq:   NP_338004.1
- ProteinModelPortal:   O50400
- EnsemblBacteria:   EBMYCT00000001278
- EnsemblBacteria:   EBMYCT00000070135
- GeneID:   888053
- GeneID:   926515
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3481
- KEGG:   mtu:Rv3371
- TIGR:   MT3481
- TubercuList:   Rv3371
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   TRLCDDA
- ProtClustDB:   CLSK792453
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 48848; Mature: 48717

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLA
CCCCCCCCHHHHHCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TEWIDYPGFDLTQHVRRVALPRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNR
HHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCE
WAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQIPPYGDARSWAETLWRMSV
EEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGR
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RQPDSLSDYTPLMLCAKMIHALARLPQQGIVTLATSAPRPRHQLRLMGQKMDQVLPIPPT
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
ALQLSTGIAVLSYGDELVFGITADYDAASEMQQLVNGIELGVARLVALSDDSVLLFTKDR
HHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCH
RKRSSRALPSAARRGRPSVPTARARH
HHHHHHCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLA
CCCCCCCHHHHHCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TEWIDYPGFDLTQHVRRVALPRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNR
HHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCE
WAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQIPPYGDARSWAETLWRMSV
EEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGR
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RQPDSLSDYTPLMLCAKMIHALARLPQQGIVTLATSAPRPRHQLRLMGQKMDQVLPIPPT
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
ALQLSTGIAVLSYGDELVFGITADYDAASEMQQLVNGIELGVARLVALSDDSVLLFTKDR
HHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCH
RKRSSRALPSAARRGRPSVPTARARH
HHHHHHCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036