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The map label for this gene is mdeA [H]

Identifier: 148824546

GI number: 148824546

Start: 3738454

End: 3739803

Strand: Direct

Name: mdeA [H]

Synonym: TBFG_13373

Alternate gene names: 148824546

Gene position: 3738454-3739803 (Clockwise)

Preceding gene: 148824543

Following gene: 148824547

Centisome position: 84.5

GC content: 66.81

Gene sequence:

>1350_bases
ATGAGCGCCGACAGCAATAGCACCGACGCCGATCCGACCGCGCATTGGTCGTTCGAAACCAAACAGATACACGCTGGTCA
GCACCCTGATCCGACCACCAACGCCCGGGCTCTGCCGATCTATGCGACCACGTCGTACACCTTCGACGACACCGCGCACG
CCGCCGCCCTGTTCGGACTGGAAATTCCGGGCAATATCTACACCCGGATCGGCAACCCCACCACCGACGTCGTCGAGCAG
CGCATCGCCGCGCTCGAGGGCGGTGTGGCCGCGCTGTTCCTGTCGTCGGGGCAGGCCGCGGAGACGTTCGCCATCTTGAA
CCTGGCCGGCGCGGGCGATCACATCGTGTCCAGCCCGCGCCTGTACGGCGGCACCTACAACCTGTTCCACTATTCGCTGG
CCAAGCTCGGCATCGAGGTCAGCTTCGTCGACGATCCGGACGATCTGGACACCTGGCAGGCGGCGGTACGGCCCAACACC
AAGGCGTTCTTCGCCGAGACCATCTCCAACCCGCAGATCGACCTGCTGGACACCCCGGCGGTTTCCGAGGTCGCCCATCG
CAACGGGGTGCCGTTGATCGTCGACAACACCATCGCCACGCCATACCTGATCCAACCGTTGGCCCAGGGCGCCGACATCG
TCGTGCATTCGGCCACCAAGTACCTGGGCGGGCACGGTGCCGCCATCGCGGGTGTGATCGTCGACGGCGGCAACTTCGAT
TGGACCCAGGGCCGCTTCCCCGGCTTCACCACCCCCGACCCCAGCTACCACGGCGTGGTGTTCGCCGAGCTGGGTCCACC
GGCGTTTGCGCTCAAAGCTCGAGTGCAGCTGCTCCGTGACTACGGCTCGGCGGCTTCGCCGTTCAACGCGTTCTTGGTGG
CGCAGGGTCTGGAAACGCTGAGCCTGCGGATCGAGCGGCACGTCGCCAACGCGCAGCGCGTCGCCGAGTTCCTGGCCGCC
CGCGACGACGTGCTTTCGGTCAACTATGCGGGGCTGCCCTCCTCGCCCTGGCATGAGCGGGCCAAGAGGCTGGCGCCCAA
GGGAACCGGGGCCGTGCTGTCCTTCGAGTTGGCCGGCGGCATCGAGGCCGGCAAGGCATTCGTGAACGCGTTGAAGCTGC
ACAGCCACGTCGCCAACATCGGTGACGTGCGCTCGCTGGTGATCCACCCGGCATCGACCACTCATGCCCAGCTGAGCCCG
GCCGAGCAGCTGGCGACCGGGGTCAGCCCGGGCCTGGTGCGTTTGGCTGTGGGCATCGAAGGTATCGACGATATCCTGGC
CGACCTGGAGCTTGGCTTTGCCGCGGCCCGCAGATTCAGCGCCGACCCGCAGTCCGTGGCGGCGTTCTGA

Upstream 100 bases:

>100_bases
GTGGGGTGCCCCGGGTGATGACCAGGTTGAGTAGCCATCGCCGGCTGCGCGGCAAGCGCGGGTCCGCCATGACGGGCCCC
TGACCAGACGGGGAAAGCTC

Downstream 100 bases:

>100_bases
GGAATTCTGACATGACGATCTCCGATGTACCCACCCAGACGCTGCCCGCCGAAGGCGAAATCGGCCTGATAGACGTCGGC
TCGCTGCAACTGGAAAGCGG

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSADSNSTDADPTAHWSFETKQIHAGQHPDPTTNARALPIYATTSYTFDDTAHAAALFGLEIPGNIYTRIGNPTTDVVEQ
RIAALEGGVAALFLSSGQAAETFAILNLAGAGDHIVSSPRLYGGTYNLFHYSLAKLGIEVSFVDDPDDLDTWQAAVRPNT
KAFFAETISNPQIDLLDTPAVSEVAHRNGVPLIVDNTIATPYLIQPLAQGADIVVHSATKYLGGHGAAIAGVIVDGGNFD
WTQGRFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDYGSAASPFNAFLVAQGLETLSLRIERHVANAQRVAEFLAA
RDDVLSVNYAGLPSSPWHERAKRLAPKGTGAVLSFELAGGIEAGKAFVNALKLHSHVANIGDVRSLVIHPASTTHAQLSP
AEQLATGVSPGLVRLAVGIEGIDDILADLELGFAAARRFSADPQSVAAF

Sequences:

>Translated_449_residues
MSADSNSTDADPTAHWSFETKQIHAGQHPDPTTNARALPIYATTSYTFDDTAHAAALFGLEIPGNIYTRIGNPTTDVVEQ
RIAALEGGVAALFLSSGQAAETFAILNLAGAGDHIVSSPRLYGGTYNLFHYSLAKLGIEVSFVDDPDDLDTWQAAVRPNT
KAFFAETISNPQIDLLDTPAVSEVAHRNGVPLIVDNTIATPYLIQPLAQGADIVVHSATKYLGGHGAAIAGVIVDGGNFD
WTQGRFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDYGSAASPFNAFLVAQGLETLSLRIERHVANAQRVAEFLAA
RDDVLSVNYAGLPSSPWHERAKRLAPKGTGAVLSFELAGGIEAGKAFVNALKLHSHVANIGDVRSLVIHPASTTHAQLSP
AEQLATGVSPGLVRLAVGIEGIDDILADLELGFAAARRFSADPQSVAAF
>Mature_448_residues
SADSNSTDADPTAHWSFETKQIHAGQHPDPTTNARALPIYATTSYTFDDTAHAAALFGLEIPGNIYTRIGNPTTDVVEQR
IAALEGGVAALFLSSGQAAETFAILNLAGAGDHIVSSPRLYGGTYNLFHYSLAKLGIEVSFVDDPDDLDTWQAAVRPNTK
AFFAETISNPQIDLLDTPAVSEVAHRNGVPLIVDNTIATPYLIQPLAQGADIVVHSATKYLGGHGAAIAGVIVDGGNFDW
TQGRFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDYGSAASPFNAFLVAQGLETLSLRIERHVANAQRVAEFLAAR
DDVLSVNYAGLPSSPWHERAKRLAPKGTGAVLSFELAGGIEAGKAFVNALKLHSHVANIGDVRSLVIHPASTTHAQLSPA
EQLATGVSPGLVRLAVGIEGIDDILADLELGFAAARRFSADPQSVAAF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=34.4913151364764, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=30.7692307692308, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI25453487, Length=402, Percent_Identity=31.0945273631841, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=34.4418052256532, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1789383, Length=424, Percent_Identity=27.8301886792453, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=413, Percent_Identity=34.3825665859564, Blast_Score=231, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=35.3808353808354, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=35.8722358722359, Blast_Score=218, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=48.4988452655889, Blast_Score=348, Evalue=9e-97,
Organism=Saccharomyces cerevisiae, GI6319307, Length=427, Percent_Identity=34.4262295081967, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6321170, Length=339, Percent_Identity=28.023598820059, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=438, Percent_Identity=26.9406392694064, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321904, Length=420, Percent_Identity=26.1904761904762, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=34.1981132075472, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47371; Mature: 47240

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADSNSTDADPTAHWSFETKQIHAGQHPDPTTNARALPIYATTSYTFDDTAHAAALFGL
CCCCCCCCCCCCCEEECEECCEEECCCCCCCCCCCEEEEEEEEECCCCCCCHHEEEEEEE
EIPGNIYTRIGNPTTDVVEQRIAALEGGVAALFLSSGQAAETFAILNLAGAGDHIVSSPR
ECCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCE
LYGGTYNLFHYSLAKLGIEVSFVDDPDDLDTWQAAVRPNTKAFFAETISNPQIDLLDTPA
EECCCHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCH
VSEVAHRNGVPLIVDNTIATPYLIQPLAQGADIVVHSATKYLGGHGAAIAGVIVDGGNFD
HHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCEEEECHHHHHCCCCCEEEEEEEECCCCC
WTQGRFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDYGSAASPFNAFLVAQGLETL
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHH
SLRIERHVANAQRVAEFLAARDDVLSVNYAGLPSSPWHERAKRLAPKGTGAVLSFELAGG
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEECCC
IEAGKAFVNALKLHSHVANIGDVRSLVIHPASTTHAQLSPAEQLATGVSPGLVRLAVGIE
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCEEEEECCC
GIDDILADLELGFAAARRFSADPQSVAAF
CHHHHHHHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
SADSNSTDADPTAHWSFETKQIHAGQHPDPTTNARALPIYATTSYTFDDTAHAAALFGL
CCCCCCCCCCCCEEECEECCEEECCCCCCCCCCCEEEEEEEEECCCCCCCHHEEEEEEE
EIPGNIYTRIGNPTTDVVEQRIAALEGGVAALFLSSGQAAETFAILNLAGAGDHIVSSPR
ECCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCE
LYGGTYNLFHYSLAKLGIEVSFVDDPDDLDTWQAAVRPNTKAFFAETISNPQIDLLDTPA
EECCCHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCH
VSEVAHRNGVPLIVDNTIATPYLIQPLAQGADIVVHSATKYLGGHGAAIAGVIVDGGNFD
HHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCEEEECHHHHHCCCCCEEEEEEEECCCCC
WTQGRFPGFTTPDPSYHGVVFAELGPPAFALKARVQLLRDYGSAASPFNAFLVAQGLETL
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHH
SLRIERHVANAQRVAEFLAARDDVLSVNYAGLPSSPWHERAKRLAPKGTGAVLSFELAGG
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEECCC
IEAGKAFVNALKLHSHVANIGDVRSLVIHPASTTHAQLSPAEQLATGVSPGLVRLAVGIE
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCEEEEECCC
GIDDILADLELGFAAARRFSADPQSVAAF
CHHHHHHHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]