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The map label for this gene is moeZ

Identifier: 148824403

GI number: 148824403

Start: 3594296

End: 3595474

Strand: Reverse

Name: moeZ

Synonym: TBFG_13230

Alternate gene names: 148824403

Gene position: 3595474-3594296 (Counterclockwise)

Preceding gene: 148824404

Following gene: 148824402

Centisome position: 81.26

GC content: 63.27

Gene sequence:

>1179_bases
GTGTCGACATCCTTGCCGCCACTAGTTGAGCCAGCATCCGCGCTCAGCCGTGAAGAGGTAGCTCGCTACAGCCGCCATCT
CATCATTCCTGACCTGGGTGTTGACGGGCAGAAGAGGCTCAAGAACGCGCGAGTACTGGTGATCGGCGCCGGCGGGCTCG
GAGCGCCCACGTTATTGTATCTGGCCGCCGCCGGCGTCGGCACCATCGGCATCGTCGACTTCGATGTGGTTGACGAGTCG
AACCTGCAGCGCCAGGTCATCCATGGGGTAGCTGACGTCGGGCGCTCCAAGGCTCAGTCGGCGCGCGATTCGATTGTCGC
GATCAACCCGCTGATCCGGGTGCGACTACACGAGCTGCGGCTTGCGCCGAGCAACGCCGTCGACCTGTTCAAGCAGTACG
ACCTGATCTTGGATGGCACCGACAACTTCGCCACCCGGTATCTGGTCAACGACGCCGCGGTGCTGGCTGGCAAGCCGTAC
GTGTGGGGGTCGATTTACCGCTTCGAGGGCCAGGCATCGGTGTTTTGGGAGGACGCCCCCGACGGGTTGGGTGTCAACTA
CCGCGACCTGTATCCCGAGCCGCCGCCGCCGGGCATGGTGCCGTCCTGTGCTGAAGGCGGGGTGCTGGGCATTATCTGTG
CCTCGGTCGCATCGGTGATGGGCACTGAGGCGATCAAGCTGATCACCGGGATAGGTGAAACACTGCTTGGCCGGTTGCTG
GTGTACGACGCGCTGGAGATGAGCTATCGCACGATCACCATCCGCAAGGACCCATCGACACCGAAGATCACCGAGCTGGT
CGACTACGAACAGTTCTGCGGCGTGGTTGCTGACGATGCCGCCCAGGCGGCCAAAGGTTCCACCATCACGCCGCGCGAGC
TGCGCGACTGGCTGGACTCCGGCCGTAAGCTGGCACTGATCGACGTTCGCGATCCCGTGGAGTGGGACATCGTGCATATC
GACGGAGCCCAACTGATTCCGAAATCGTTGATCAACTCGGGTGAGGGTCTGGCCAAGCTCCCCCAGGACCGCACCGCCGT
GCTGTACTGCAAGACGGGCGTGCGCTCGGCCGAGGCATTGGCTGCGGTCAAGAAAGCCGGTTTCTCCGACGCGGTACACC
TGCAGGGTGGGATCGTCGCGTGGGCCAAGCAGATGCAGCCCGACATGGTGATGTACTAG

Upstream 100 bases:

>100_bases
GATTCCCTAACCCGGGGAAGCAAAGGTGGCAAGCTGGGCGTTGAGGTATTTGCAGGACGACGCGCAGCAGACGTTTCGGA
TATAACCGAGGAGATATTCG

Downstream 100 bases:

>100_bases
GACCGATACAGGCCCAATACACCCCGATGGGCTCTACTAGGCTGACCGGTGTGAATGTCGAGCCGCCGCCCGAGCACGTG
TTGGTGGCGTTCGGTTTGGC

Product: molybdopterin biosynthesis-like protein MoeZ

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSTSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDFDVVDES
NLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPY
VWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLL
VYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDSGRKLALIDVRDPVEWDIVHI
DGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEALAAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY

Sequences:

>Translated_392_residues
MSTSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDFDVVDES
NLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPY
VWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLL
VYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDSGRKLALIDVRDPVEWDIVHI
DGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEALAAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY
>Mature_391_residues
STSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDFDVVDESN
LQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPYV
WGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLLV
YDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDSGRKLALIDVRDPVEWDIVHID
GAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEALAAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Homo sapiens, GI7657339, Length=416, Percent_Identity=39.4230769230769, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI4885649, Length=132, Percent_Identity=36.3636363636364, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI38045944, Length=114, Percent_Identity=35.9649122807018, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI38045942, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=37.9591836734694, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082356, Length=250, Percent_Identity=36, Blast_Score=147, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17540406, Length=404, Percent_Identity=38.6138613861386, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI193203301, Length=141, Percent_Identity=31.2056737588652, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984476, Length=185, Percent_Identity=30.2702702702703, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321903, Length=395, Percent_Identity=39.4936708860759, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6320598, Length=148, Percent_Identity=30.4054054054054, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322639, Length=217, Percent_Identity=24.4239631336406, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325438, Length=123, Percent_Identity=29.2682926829268, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=403, Percent_Identity=38.2133995037221, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24660640, Length=139, Percent_Identity=35.9712230215827, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28573937, Length=149, Percent_Identity=28.1879194630872, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129973, Length=108, Percent_Identity=36.1111111111111, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOEZ_MYCTU (Q7D5X9)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   G70594
- RefSeq:   NP_337830.1
- RefSeq:   YP_177942.1
- HSSP:   P12282
- ProteinModelPortal:   Q7D5X9
- SMR:   Q7D5X9
- EnsemblBacteria:   EBMYCT00000003105
- EnsemblBacteria:   EBMYCT00000072500
- GeneID:   888871
- GeneID:   923277
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3301
- KEGG:   mtu:Rv3206c
- TIGR:   MT3301
- TubercuList:   Rv3206c
- GeneTree:   EBGT00050000015471
- HOGENOM:   HBG535305
- OMA:   IHGTSWV
- ProtClustDB:   PRK07878
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF; SSF69572 MoeB

EC number: NA

Molecular weight: Translated: 42174; Mature: 42043

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 203-203 ACT_SITE 350-350 BINDING 52-52 BINDING 73-73 BINDING 97-97

Signals:

None

Transmembrane regions:

HASH(0x1c1755f8)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLY
CCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHCCCEEEEEECCCCCHHHHHH
LAAAGVGTIGIVDFDVVDESNLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELR
HHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHEEHHHC
LAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAP
CCCCHHHHHHHHCCEEEECCCCHHEEEEECCCCEECCCCCEEEEEEEECCCEEEEECCCC
DGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDS
HHHHHHCCEEEEEEEECCCCCHHHHHHCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHC
GRKLALIDVRDPVEWDIVHIDGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEAL
CCEEEEEECCCCCEEEEEEECCHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCHHHHH
AAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY
HHHHHCCCCCEEEECCCHHHHHHHCCCCCCCH
>Mature Secondary Structure 
STSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLY
CCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCHHHHHCCCEEEEEECCCCCHHHHHH
LAAAGVGTIGIVDFDVVDESNLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELR
HHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHEEHHHC
LAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAP
CCCCHHHHHHHHCCEEEECCCCHHEEEEECCCCEECCCCCEEEEEEEECCCEEEEECCCC
DGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDS
HHHHHHCCEEEEEEEECCCCCHHHHHHCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHC
GRKLALIDVRDPVEWDIVHIDGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEAL
CCEEEEEECCCCCEEEEEEECCHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCHHHHH
AAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY
HHHHHCCCCCEEEECCCHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036