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The map label for this gene is nuoM [H]

Identifier: 148824351

GI number: 148824351

Start: 3537255

End: 3538916

Strand: Direct

Name: nuoM [H]

Synonym: TBFG_13178

Alternate gene names: 148824351

Gene position: 3537255-3538916 (Clockwise)

Preceding gene: 148824350

Following gene: 148824352

Centisome position: 79.95

GC content: 64.26

Gene sequence:

>1662_bases
GTGAATAACGTGCCGTGGCTGAGCGTGCTCTGGCTGGTGCCGCTGGCAGGTGCGGTGCTGATCATCCTGCTACCACCCGG
TCGGCGCCGACTCGCCAAGTGGGCCGGTATGGTTGTCAGCGTCCTGACGTTGGCGGTGTCGATCGTCGTCGCGGCCGAAT
TCAAGCCCAGCGCCGAGCCGTATCAGTTCGTCGAAAAGCATTCCTGGATACCGGCGTTCGGCGCCGGCTATACCCTTGGT
GTGGACGGCATCGCAGTGGTGCTGGTGTTGTTGACCACAGTGCTGATTCCGTTGCTGCTGGTGGCCGGCTGGAACGACGC
AACCGATGCTGACGACCTGTCCCCCGCAAGCGGGAGGTACCCCCAGCGCCCGGCTCCGCCGCGCTTGCGATCGTCAGGTG
GCGAACGCACCCGAGGCGTGCACGCCTACGTGGCATTGACGCTGGCCATCGAGTCGATGGTGCTGATGTCGGTGATCGCG
CTGGACGTGCTGCTGTTCTACGTGTTCTTCGAGGCCATGCTGATCCCGATGTACTTCCTCATCGGCGGCTTCGGCCAGGG
GGCCGGACGCTCGCGTGCCGCGGTGAAGTTCTTGCTGTACAACCTGTTTGGCGGGTTGATCATGCTGGCGGCGGTGATCG
GGCTGTATGTGGTGACCGCACAGTACGATTCGGGCACCTTCGACTTCCGTGAGATCGTGGCCGGCGTGGCGGCGGGCCGC
TACGGAGCGGACCCGGCGGTGTTCAAGGCGCTGTTCTTGGGCTTCATGTTCACGTTCGCGATCAAGGCTCCGCTGTGGCC
GTTCCATCGCTGGCTGCCGGACGCCGCCGTCGAGTCCACCCCAGCGACCGCGGTGCTGATGATGGCGGTGATGGACAAGG
TCGGCACCTTCGGCATGCTGCGCTACTGCCTGCAGCTGTTTCCTGACCCGTCAACGTATTTCCGTCCGCTGATCGTGACG
CTGGCCATCATCGGGGTGATCTACGGCGCGATCGTGGCGATCGGCCAAACCGACATGATGCGGCTGATCGCCTACACCTC
GATCTCGCACTTCGGGTTCATCATCGCAGGCATCTTCGTCATGACCACCCAGGGCCAGAGCGGGTCGACGCTGTACATGC
TCAACCACGGCCTGTCCACGGCGGCGGTGTTCCTGATCGCCGGTTTCTTGATAGCGCGGCGCGGCAGCCGATCGATCGCC
GACTACGGCGGTGTCCAGAAGGTGGCGCCCATCCTGGCCGGCACGTTCATGGTCTCGGCCATGGCCACCGTATCGCTGCC
CGGCCTAGCCCCGTTTATCAGCGAATTCCTGGTTCTGCTGGGCACTTTCAGCCGCTACTGGCTGGCGGCGGCGTTCGGCG
TTACCGCACTGGTCCTCTCGGCCGTTTACATGCTGTGGCTCTACCAGCGGGTGATGACCGGTCCGGTAGCCGAAGGCAAC
GAACGCATAGGGGATCTGGTGGGCCGCGAGATGATCGTGGTGGCACCGTTGATCGCGCTGTTACTCGTGCTTGGGGTCTA
CCCCAAACCTGTGCTCGACATCATCAATCCGGCGGTCGAGAACACCATGACCACCATCGGCCAGCATGATCCCGCGCCCA
GCGTGGCACACCCGGTTCCGGCCGTGGGCGCCTCCCGGACAGCCGAAGGACCGCACCCATGA

Upstream 100 bases:

>100_bases
GCCTGCGGCAAATGCAAACCGGCTTCGCCCGTAACTACGCGTTATCGATGCTGGTAGGAGCGGTGTTAGTGGCGGCGGCG
CTGCTGGTGGTGCAGCTGTG

Downstream 100 bases:

>100_bases
TCCTGCCCGCCCCGCACGTCGAGTACTTCCTGCTCGCTCCGATGCTCATCGTCTTTTCGGTTGCGGTCGCCGGTGTGCTG
GCCGAGGCTTTCCTGCCGCG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEPYQFVEKHSWIPAFGAGYTLG
VDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRYPQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIA
LDVLLFYVFFEAMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
YGADPAVFKALFLGFMFTFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGMLRYCLQLFPDPSTYFRPLIVT
LAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFVMTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIA
DYGGVQKVAPILAGTFMVSAMATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVPAVGASRTAEGPHP

Sequences:

>Translated_553_residues
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEPYQFVEKHSWIPAFGAGYTLG
VDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRYPQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIA
LDVLLFYVFFEAMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
YGADPAVFKALFLGFMFTFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGMLRYCLQLFPDPSTYFRPLIVT
LAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFVMTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIA
DYGGVQKVAPILAGTFMVSAMATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVPAVGASRTAEGPHP
>Mature_553_residues
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEPYQFVEKHSWIPAFGAGYTLG
VDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRYPQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIA
LDVLLFYVFFEAMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
YGADPAVFKALFLGFMFTFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGMLRYCLQLFPDPSTYFRPLIVT
LAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFVMTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIA
DYGGVQKVAPILAGTFMVSAMATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVPAVGASRTAEGPHP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=388, Percent_Identity=27.8350515463918, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI251831117, Length=351, Percent_Identity=21.6524216524217, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788613, Length=534, Percent_Identity=31.4606741573034, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=26.2820512820513, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788831, Length=410, Percent_Identity=24.8780487804878, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788829, Length=508, Percent_Identity=24.2125984251968, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693160, Length=368, Percent_Identity=26.6304347826087, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788614, Length=362, Percent_Identity=26.5193370165746, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI2367154, Length=293, Percent_Identity=26.962457337884, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 59238; Mature: 59238

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00673 V8_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEP
CCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YQFVEKHSWIPAFGAGYTLGVDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRY
HHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIALDVLLFYVFFEAMLIPMYFL
CCCCCCCCHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCC
YGADPAVFKALFLGFMFTFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGML
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
RYCLQLFPDPSTYFRPLIVTLAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
MTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIADYGGVQKVAPILAGTFMVSA
HEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHH
MATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AVGASRTAEGPHP
CCCCCCCCCCCCC
>Mature Secondary Structure
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEP
CCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YQFVEKHSWIPAFGAGYTLGVDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRY
HHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIALDVLLFYVFFEAMLIPMYFL
CCCCCCCCHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCC
YGADPAVFKALFLGFMFTFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGML
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
RYCLQLFPDPSTYFRPLIVTLAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
MTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIADYGGVQKVAPILAGTFMVSA
HEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHH
MATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AVGASRTAEGPHP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]