Click here to switch to the map view.

The map label for this gene is dinB2

Identifier: 148824248

GI number: 148824248

Start: 3428401

End: 3429441

Strand: Direct

Name: dinB2

Synonym: TBFG_13073

Alternate gene names: 148824248

Gene position: 3428401-3429441 (Clockwise)

Preceding gene: 148824247

Following gene: 148824251

Centisome position: 77.49

GC content: 62.92

Gene sequence:

>1041_bases
ATGCCGACAGCAGCACCGCGATGGATCCTGCACGTAGACCTCGACCAGTTTTTGGCGTCGGTCGAACTGCTCCGCCACCC
CGAACTGGCAGGTTTGCCGGTCATCGTCGGCGGCAACGGTGATCCGACCGAACCGCGAAAGGTCGTCACCTGTGCGTCGT
ATGAGGCCCGCGCCTACGGTGTGCGCGCCGGCATGCCGTTGCGGACCGCCGCCCGACGATGCCCTGAGGCCACCTTCTTG
CCGTCGAACCCAGCCGCCTACAACGCGGCGTCCGAGGAGGTGGTGGCGTTATTGCGCGACCTGGGATACCCGGTCGAGGT
ATGGGGCTGGGACGAGGCTTACCTCGCGGTGGCGCCCGGGACTCCCGACGACCCCATCGAAGTCGCCGAAGAGATCCGAA
AAGTCATCTTGTCGCAAACCGGGCTGTCTTGCTCGATAGGTATCAGTGACAACAAGCAGCGCGCCAAGATCGCTACCGGG
TTGGCGAAACCAGCTGGCATCTATCAGCTCACCGATGCCAACTGGATGGCCATCATGGGTGACCGTACCGTCGAAGCACT
GTGGGGTGTGGGGCCCAAGACTACGAAAAGGCTGGCAAAGCTTGGGATCAACACCGTTTACCAACTTGCACACACCGATT
CCGGGCTATTGATGTCCACGTTCGGTCCGCGAACCGCGCTGTGGCTGCTGCTGGCCAAAGGCGGAGGCGATACCGAAGTC
AGTGCCCAAGCTTGGGTTCCACGCTCGCGCAGCCACGCCGTCACCTTTCCACGAGACCTCACCTGCCGATCCGAAATGGA
ATCGGCCGTGACGGAATTGGCGCAGCGAACACTCAACGAGGTGGTGGCTTCGTCGCGAACCGTCACCCGAGTCGCGGTCA
CCGTGCGCACGGCGACGTTCTACACCCGCACCAAGATCCGAAAGCTGCAAGCTCCCAGCACCGATCCCGACGTCATCACC
GCTGCCGCCCGGCACGTTCTTGACCTATTCGAGCTGGATCGGCCCGTCCGGTTGCTGGGAGTGCGGTTAGAACTGGCCTA
G

Upstream 100 bases:

>100_bases
TGTCGAGCACCAACTCAACGCCGGCGGCCATACCCTGCAAACCCTGGGCGACGCATGGGAGAGCCTGGCGCGAAAACTGT
GCGGACGATGATCGATCACT

Downstream 100 bases:

>100_bases
AACCGGCGGGCACACCGCACCTGGGCGGCGCGAAGTCTTGACCGCACCGGCCGCTATGGCCCGGGCCGAAGCGCGCGCGT
GAAGAACACGTTGACTCGTC

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 2

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPTAAPRWILHVDLDQFLASVELLRHPELAGLPVIVGGNGDPTEPRKVVTCASYEARAYGVRAGMPLRTAARRCPEATFL
PSNPAAYNAASEEVVALLRDLGYPVEVWGWDEAYLAVAPGTPDDPIEVAEEIRKVILSQTGLSCSIGISDNKQRAKIATG
LAKPAGIYQLTDANWMAIMGDRTVEALWGVGPKTTKRLAKLGINTVYQLAHTDSGLLMSTFGPRTALWLLLAKGGGDTEV
SAQAWVPRSRSHAVTFPRDLTCRSEMESAVTELAQRTLNEVVASSRTVTRVAVTVRTATFYTRTKIRKLQAPSTDPDVIT
AAARHVLDLFELDRPVRLLGVRLELA

Sequences:

>Translated_346_residues
MPTAAPRWILHVDLDQFLASVELLRHPELAGLPVIVGGNGDPTEPRKVVTCASYEARAYGVRAGMPLRTAARRCPEATFL
PSNPAAYNAASEEVVALLRDLGYPVEVWGWDEAYLAVAPGTPDDPIEVAEEIRKVILSQTGLSCSIGISDNKQRAKIATG
LAKPAGIYQLTDANWMAIMGDRTVEALWGVGPKTTKRLAKLGINTVYQLAHTDSGLLMSTFGPRTALWLLLAKGGGDTEV
SAQAWVPRSRSHAVTFPRDLTCRSEMESAVTELAQRTLNEVVASSRTVTRVAVTVRTATFYTRTKIRKLQAPSTDPDVIT
AAARHVLDLFELDRPVRLLGVRLELA
>Mature_345_residues
PTAAPRWILHVDLDQFLASVELLRHPELAGLPVIVGGNGDPTEPRKVVTCASYEARAYGVRAGMPLRTAARRCPEATFLP
SNPAAYNAASEEVVALLRDLGYPVEVWGWDEAYLAVAPGTPDDPIEVAEEIRKVILSQTGLSCSIGISDNKQRAKIATGL
AKPAGIYQLTDANWMAIMGDRTVEALWGVGPKTTKRLAKLGINTVYQLAHTDSGLLMSTFGPRTALWLLLAKGGGDTEVS
AQAWVPRSRSHAVTFPRDLTCRSEMESAVTELAQRTLNEVVASSRTVTRVAVTVRTATFYTRTKIRKLQAPSTDPDVITA
AARHVLDLFELDRPVRLLGVRLELA

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain

Homologues:

Organism=Homo sapiens, GI84043967, Length=342, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI7706681, Length=343, Percent_Identity=26.2390670553936, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI7705344, Length=109, Percent_Identity=37.6146788990826, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786425, Length=344, Percent_Identity=29.0697674418605, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787432, Length=351, Percent_Identity=26.7806267806268, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17537959, Length=344, Percent_Identity=27.3255813953488, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19923006, Length=344, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO42_MYCBO (P63988)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856727.1
- ProteinModelPortal:   P63988
- SMR:   P63988
- EnsemblBacteria:   EBMYCT00000017096
- GeneID:   1093596
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3082
- GeneTree:   EBGT00050000015928
- HOGENOM:   HBG734504
- OMA:   IIAHIDI
- ProtClustDB:   PRK03352
- BioCyc:   MBOV233413:MB3082-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- HAMAP:   MF_01113
- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880
- Gene3D:   G3DSA:3.30.1490.100
- PANTHER:   PTHR11076

Pfam domain/function: PF00817 IMS; SSF100879 DNA_pol_Y-fam_little_finger

EC number: =2.7.7.7

Molecular weight: Translated: 37563; Mature: 37431

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50173 UMUC

Important sites: ACT_SITE 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTAAPRWILHVDLDQFLASVELLRHPELAGLPVIVGGNGDPTEPRKVVTCASYEARAYG
CCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCEEE
VRAGMPLRTAARRCPEATFLPSNPAAYNAASEEVVALLRDLGYPVEVWGWDEAYLAVAPG
ECCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCC
TPDDPIEVAEEIRKVILSQTGLSCSIGISDNKQRAKIATGLAKPAGIYQLTDANWMAIMG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEECCCCEEEEEC
DRTVEALWGVGPKTTKRLAKLGINTVYQLAHTDSGLLMSTFGPRTALWLLLAKGGGDTEV
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHEEEEEEECCCCCCCC
SAQAWVPRSRSHAVTFPRDLTCRSEMESAVTELAQRTLNEVVASSRTVTRVAVTVRTATF
CHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
YTRTKIRKLQAPSTDPDVITAAARHVLDLFELDRPVRLLGVRLELA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEC
>Mature Secondary Structure 
PTAAPRWILHVDLDQFLASVELLRHPELAGLPVIVGGNGDPTEPRKVVTCASYEARAYG
CCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCEEE
VRAGMPLRTAARRCPEATFLPSNPAAYNAASEEVVALLRDLGYPVEVWGWDEAYLAVAPG
ECCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCC
TPDDPIEVAEEIRKVILSQTGLSCSIGISDNKQRAKIATGLAKPAGIYQLTDANWMAIMG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEECCCCEEEEEC
DRTVEALWGVGPKTTKRLAKLGINTVYQLAHTDSGLLMSTFGPRTALWLLLAKGGGDTEV
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHEEEEEEECCCCCCCC
SAQAWVPRSRSHAVTFPRDLTCRSEMESAVTELAQRTLNEVVASSRTVTRVAVTVRTATF
CHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHH
YTRTKIRKLQAPSTDPDVITAAARHVLDLFELDRPVRLLGVRLELA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972