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The map label for this gene is wcaL [C]

Identifier: 148824223

GI number: 148824223

Start: 3403244

End: 3404488

Strand: Direct

Name: wcaL [C]

Synonym: TBFG_13048

Alternate gene names: 148824223

Gene position: 3403244-3404488 (Clockwise)

Preceding gene: 148824222

Following gene: 148824224

Centisome position: 76.92

GC content: 68.03

Gene sequence:

>1245_bases
ATGAGGATCCTCATGGTGTCGTGGGAGTACCCGCCGGTGGTGATCGGCGGACTCGGCCGCCACGTGCATCATCTGTCGAC
CGCGCTAGCCGCAGCCGGTCACGATGTCGTCGTGTTGTCCCGGTGTCCGTCGGGCACCGATCCCAGCACACACCCATCCT
CCGATGAGGTGACCGAAGGGGTCCGGGTGATTGCGGCCGCGCAGGACCCGCACGAGTTCACGTTTGGCAACGACATGATG
GCCTGGACCCTGGCGATGGGCCACGCCATGATCCGCGCCGGGCTGCGCTTGAAGAAACTTGGCACCGACCGCTCGTGGCG
TCCTGACGTCGTGCACGCACACGACTGGCTGGTGGCCCATCCGGCCATCGCCCTTGCCCAGTTCTATGACGTGCCAATGG
TTTCCACGATTCATGCAACGGAGGCCGGTCGACATTCCGGCTGGGTCTCCGGAGCTCTCAGCCGTCAGGTGCACGCGGTC
GAGTCGTGGCTGGTGCGTGAATCCGATTCGCTGATCACATGCTCGGCGTCGATGAACGACGAGATCACCGAGCTGTTCGG
GCCCGGGCTGGCCGAGATCACCGTGATCCGTAACGGCATTGACGCGGCGCGCTGGCCGTTCGCGGCCCGCCGCCCGCGCA
CCGGGCCAGCCGAATTGCTCTATGTGGGGCGGCTGGAGTACGAGAAGGGCGTGCACGACGCCATCGCCGCGCTGCCGCGG
CTCAGGCGCACTCACCCAGGCACCACACTGACCATCGCCGGCGAAGGCACCCAGCAGGATTGGTTGATCGATCAGGCCCG
CAAACACCGGGTGCTCAGAGCAACCAGGTTCGTCGGACACCTCGACCACACCGAGCTGCTGGCGTTGCTGCACCGAGCCG
ACGCCGCGGTGCTGCCCAGCCACTACGAACCGTTTGGGCTGGTGGCACAGGAGGCCGCCGCGGCCGGCACCCCGCTGGTG
ACGTCCAACATCGGCGGTCTGGGTGAAGCGGTCATCAATGGACAGACCGGGGTGTCGTGTGCACCCCGCGACGTAGCGGG
GCTGGCCGCCGCGGTGCGTAGCGTGCTCGACGATCCGGCCGCCGCGCAGCGGCGCGCACGAGCCGCCCGGCAACGGCTCA
CCTCCGACTTCGACTGGCAGACGGTGGCCACCGCGACCGCGCAGGTGTACCTGGCGGCGAAGCGCGGTGAACGGCAGCCG
CAGCCCCGGTTGCCCATCGTCGAGCACGCTCTTCCCGATCGGTAG

Upstream 100 bases:

>100_bases
CTCGCCGAAGGGTGGAACCGCGCCGACGGTCTGTTCGGCGCCCTGGACGCTCGGAGGCTGCCCAAGTGAACGCCTCGCAC
AGGCGGAACCGGCCGCGCGC

Downstream 100 bases:

>100_bases
CCGTGGCAGGGACGTGATGATCGGAGCACCGCAGTGAAACCGCAGGACCAGGGGCTCCACTTCCCCTATCGCTACGACCT
TCGACTGGCGCCTATGTGGC

Product: transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEGVRVIAAAQDPHEFTFGNDMM
AWTLAMGHAMIRAGLRLKKLGTDRSWRPDVVHAHDWLVAHPAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAV
ESWLVRESDSLITCSASMNDEITELFGPGLAEITVIRNGIDAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPR
LRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPSHYEPFGLVAQEAAAAGTPLV
TSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPAAAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQP
QPRLPIVEHALPDR

Sequences:

>Translated_414_residues
MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEGVRVIAAAQDPHEFTFGNDMM
AWTLAMGHAMIRAGLRLKKLGTDRSWRPDVVHAHDWLVAHPAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAV
ESWLVRESDSLITCSASMNDEITELFGPGLAEITVIRNGIDAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPR
LRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPSHYEPFGLVAQEAAAAGTPLV
TSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPAAAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQP
QPRLPIVEHALPDR
>Mature_414_residues
MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEGVRVIAAAQDPHEFTFGNDMM
AWTLAMGHAMIRAGLRLKKLGTDRSWRPDVVHAHDWLVAHPAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAV
ESWLVRESDSLITCSASMNDEITELFGPGLAEITVIRNGIDAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPR
LRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPSHYEPFGLVAQEAAAAGTPLV
TSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPAAAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQP
QPRLPIVEHALPDR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17532709, Length=400, Percent_Identity=21.75, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI9755344, Length=402, Percent_Identity=23.3830845771144, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24654571, Length=341, Percent_Identity=26.9794721407625, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44820; Mature: 44820

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEG
CEEEEEECCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHCC
VRVIAAAQDPHEFTFGNDMMAWTLAMGHAMIRAGLRLKKLGTDRSWRPDVVHAHDWLVAH
EEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCHHHHH
PAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMND
HHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
EITELFGPGLAEITVIRNGIDAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPR
HHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCCEEEEECCCHHCCHHHHHHHHHH
LRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPS
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
HYEPFGLVAQEAAAAGTPLVTSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPA
CCCCHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHH
AAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQPQPRLPIVEHALPDR
HHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEECCCCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEG
CEEEEEECCCCCEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHCC
VRVIAAAQDPHEFTFGNDMMAWTLAMGHAMIRAGLRLKKLGTDRSWRPDVVHAHDWLVAH
EEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCHHHHH
PAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMND
HHHHHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
EITELFGPGLAEITVIRNGIDAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPR
HHHHHHCCCCEEEEEECCCCCHHHCCCCCCCCCCCCCCEEEEECCCHHCCHHHHHHHHHH
LRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPS
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
HYEPFGLVAQEAAAAGTPLVTSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPA
CCCCHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHH
AAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQPQPRLPIVEHALPDR
HHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEECCCCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]