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The map label for this gene is yqhE [C]

Identifier: 148824161

GI number: 148824161

Start: 3337574

End: 3338422

Strand: Direct

Name: yqhE [C]

Synonym: TBFG_12986

Alternate gene names: 148824161

Gene position: 3337574-3338422 (Clockwise)

Preceding gene: 148824154

Following gene: 148824165

Centisome position: 75.44

GC content: 63.72

Gene sequence:

>849_bases
GTGACTGGCGAGTCGGGCGCCGCCGCCGCACCCTCGATTACCCTCAACGACGAGCATACGATGCCGGTGCTTGGCCTCGG
CGTCGCGGAATTGTCGGACGACGAGACCGAACGTGCGGTGTCCGCGGCGCTGGAAATTGGCTGCCGGCTGATCGACACCG
CCTACGCCTATGGCAACGAGGCCGCGGTCGGCCGCGCAATTGCAGCCTCCGGCGTTGCCCGCGAAGAGCTGTTCGTCACC
ACCAAGCTAGCCACCCCCGACCAGGGTTTCACCCGTTCCCAGGAAGCATGTAGAGCCAGTTTGGACCGCCTCGGCCTCGA
CTACGTCGACCTTTACCTAATTCACTGGCCGGCCCCGCCGGTGGGCAAGTATGTGGACGCCTGGGGAGGCATGATTCAAT
CCCGCGGAGAGGGCCATGCCCGATCGATCGGCGTGTCCAACTTCACCGCGGAGAACATCGAAAACCTTATCGACCTCACA
TTCGTCACGCCGGCGGTCAACCAGATCGAGCTGCACCCGCTGCTCAACCAGGACGAACTGCGCAAAGCTAACGCCCAGCA
CACCGTCGTCACACAGTCCTACTGCCCCCTGGCACTCGGCAGGCTGCTGGACAACCCAACCGTCACATCAATCGCCAGCG
AATACGTCAAGACGCCCGCACAAGTGCTGCTGCGGTGGAACCTGCAATTGGGCAATGCGGTGGTCGTCCGCTCGGCCAGA
CCCGAGCGCATCGCCAGCAACTTCGACGTCTTCGACTTCGAGTTGGCGGCCGAACACATGGATGCATTGGGCGGGCTCAA
TGACGGCACCCGGGTGCGCGAGGATCCACTGACCTACGCCGGCACCTGA

Upstream 100 bases:

>100_bases
TTGCGAGCAACTTCGACGCGTTCGATTTCCCTCGCTCGATGACGGAACCCGGCTTGGTCCGAATCCGAAAACCTTCAATT
TCACAGGCAGGTGAGATGAC

Downstream 100 bases:

>100_bases
TACGCCGCCGACTGTGAACCGCGCGACGTCTCCTCGGCGTGTCACGTCGTGAGATTCACCGTCGGCGCGTGGACTAGCCC
GTCGGGCAGGTGGCCGCGGC

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNEAAVGRAIAASGVAREELFVT
TKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPPVGKYVDAWGGMIQSRGEGHARSIGVSNFTAENIENLIDLT
FVTPAVNQIELHPLLNQDELRKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT

Sequences:

>Translated_282_residues
MTGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNEAAVGRAIAASGVAREELFVT
TKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPPVGKYVDAWGGMIQSRGEGHARSIGVSNFTAENIENLIDLT
FVTPAVNQIELHPLLNQDELRKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT
>Mature_281_residues
TGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNEAAVGRAIAASGVAREELFVTT
KLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPPVGKYVDAWGGMIQSRGEGHARSIGVSNFTAENIENLIDLTF
VTPAVNQIELHPLLNQDELRKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSARP
ERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family

Homologues:

Organism=Homo sapiens, GI24497577, Length=296, Percent_Identity=33.7837837837838, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI5174391, Length=296, Percent_Identity=33.7837837837838, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=36.7857142857143, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=36.5771812080537, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=36.241610738255, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=36.241610738255, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=36.241610738255, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=35.7859531772575, Blast_Score=158, Evalue=4e-39,
Organism=Homo sapiens, GI93277124, Length=306, Percent_Identity=34.3137254901961, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI223468663, Length=296, Percent_Identity=33.7837837837838, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4502049, Length=300, Percent_Identity=36.6666666666667, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=34.6938775510204, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5174695, Length=311, Percent_Identity=33.4405144694534, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI291291012, Length=274, Percent_Identity=32.8467153284672, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI300116273, Length=287, Percent_Identity=33.1010452961672, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI300116271, Length=291, Percent_Identity=32.9896907216495, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=34.1935483870968, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI207028673, Length=112, Percent_Identity=44.6428571428571, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=44.6428571428571, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082198, Length=271, Percent_Identity=47.6014760147601, Blast_Score=260, Evalue=9e-71,
Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=38.1322957198444, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1788081, Length=272, Percent_Identity=30.1470588235294, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71998625, Length=267, Percent_Identity=36.3295880149813, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17537077, Length=294, Percent_Identity=34.6938775510204, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=35.0340136054422, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17552492, Length=277, Percent_Identity=34.6570397111913, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=33.1103678929766, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=32.7586206896552, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17550248, Length=297, Percent_Identity=34.3434343434343, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17561298, Length=277, Percent_Identity=32.129963898917, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17537079, Length=301, Percent_Identity=29.9003322259136, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17538386, Length=280, Percent_Identity=30.7142857142857, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17561300, Length=279, Percent_Identity=30.8243727598566, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17562292, Length=278, Percent_Identity=28.7769784172662, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320576, Length=274, Percent_Identity=39.051094890511, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324694, Length=285, Percent_Identity=36.140350877193, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322556, Length=258, Percent_Identity=32.9457364341085, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=34.6938775510204, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6321896, Length=315, Percent_Identity=30.1587301587302, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320079, Length=284, Percent_Identity=30.6338028169014, Blast_Score=128, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24663317, Length=297, Percent_Identity=37.7104377104377, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=38.2758620689655, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI20129731, Length=291, Percent_Identity=38.1443298969072, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=36.3333333333333, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI21356425, Length=307, Percent_Identity=35.5048859934853, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=34.375, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI45553081, Length=296, Percent_Identity=34.7972972972973, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24662789, Length=298, Percent_Identity=34.2281879194631, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24662785, Length=288, Percent_Identity=35.0694444444444, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=35, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=31.9230769230769, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2971_MYCTU (P95124)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   H70671
- RefSeq:   NP_217487.1
- RefSeq:   NP_337561.1
- HSSP:   P06632
- ProteinModelPortal:   P95124
- SMR:   P95124
- EnsemblBacteria:   EBMYCT00000002584
- EnsemblBacteria:   EBMYCT00000070348
- GeneID:   887275
- GeneID:   925221
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3049
- KEGG:   mtu:Rv2971
- TIGR:   MT3049
- TubercuList:   Rv2971
- GeneTree:   EBGT00050000017291
- HOGENOM:   HBG605727
- OMA:   HNVVTQS
- ProtClustDB:   CLSK792199
- GO:   GO:0040007
- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210
- Gene3D:   G3DSA:3.20.20.100
- PANTHER:   PTHR11732
- PIRSF:   PIRSF000097
- PRINTS:   PR00069

Pfam domain/function: PF00248 Aldo_ket_red; SSF51430 Aldo/ket_red

EC number: NA

Molecular weight: Translated: 30364; Mature: 30233

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1; PS00062 ALDOKETO_REDUCTASE_2; PS00063 ALDOKETO_REDUCTASE_3

Important sites: ACT_SITE 57-57 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNE
CCCCCCCCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AAVGRAIAASGVAREELFVTTKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPP
HHHHHHHHHCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VGKYVDAWGGMIQSRGEGHARSIGVSNFTAENIENLIDLTFVTPAVNQIELHPLLNQDEL
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHEECCCCCEEEECCCCCHHHH
RKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
HHCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCHHHEEEEECEEECCEEEEECCC
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT
CHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNE
CCCCCCCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AAVGRAIAASGVAREELFVTTKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPP
HHHHHHHHHCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VGKYVDAWGGMIQSRGEGHARSIGVSNFTAENIENLIDLTFVTPAVNQIELHPLLNQDEL
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHEECCCCCEEEECCCCCHHHH
RKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
HHCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCHHHEEEEECEEECCEEEEECCC
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT
CHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036