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The map label for this gene is purU

Identifier: 148824153

GI number: 148824153

Start: 3328002

End: 3328934

Strand: Direct

Name: purU

Synonym: TBFG_12978

Alternate gene names: 148824153

Gene position: 3328002-3328934 (Clockwise)

Preceding gene: 148824152

Following gene: 148824154

Centisome position: 75.22

GC content: 62.27

Gene sequence:

>933_bases
ATGGGCAAAGGGAGCATGACCGCGCACGCAACGCCGAACGAGCCGGATTATCCGCCACCGCCTGGCGGTCCACCGCCGCC
GGCCGATATTGGCCGGTTACTGCTTCGGTGCCACGACCGCCCTGGAATCATCGCCGCGGTGAGCACCTTCCTGGCCCGGG
CCGGCGCCAACATCATTTCTCTGGACCAGCACTCCACCGCGCCGGAGGGCGGAACGTTCTTGCAGCGCGCAATCTTTCAC
CTGCCCGGTCTCACGGCCGCCGTCGACGAACTGCAGCGCGACTTCGGCAGCACTGTGGCGGACAAGTTCGGCATCGACTA
CCGATTTGCCGAAGCAGCCAAGCCTAAGCGGGTCGCAATCATGGCATCGACAGAGGACCACTGCTTGCTGGACTTGTTGT
GGCGCAACCGTCGCGGCGAGCTAGAAATGTCGGTTGTCATGGTGATTGCCAATCATCCTGACCTGGCCGCGCACGTACGC
CCGTTCGGTGTGCCATTCATACATATTCCCGCCACTCGCGACACTCGTACGGAAGCCGAACAGCGTCAGCTTCAGTTGCT
AAGCGGCAATGTGGATTTAGTAGTGCTGGCACGCTACATGCAGATACTCAGCCCGGGGTTCTTGGAGGCGATCGGCTGCC
CGCTGATCAACATTCACCATTCGTTCCTTCCAGCCTTCACCGGCGCGGCCCCGTACCAGCGCGCACGAGAACGCGGCGTC
AAACTGATCGGCGCGACCGCCCACTACGTGACCGAAGTTCTCGACGAGGGGCCCATCATCGAACAAGACGTCGTTCGTGT
CGACCACACCCACACCGTCGATGATCTGGTGCGTGTCGGCGCCGACGTCGAACGCGCAGTGCTTTCCCGCGCCGTGCTCT
GGCACTGCCAAGACCGCGTCATCGTGCATCACAACCAGACCATCGTCTTCTGA

Upstream 100 bases:

>100_bases
CCGCCACGACGATCACCTCGGCCACTAGCGCCACCACGCCGATCAGTCGGCGCCGAAAAGGCCACCGGCGGCGGTATCCT
GGCCTGCGGGTATTCCACCC

Downstream 100 bases:

>100_bases
CATGGGTGACTGCGCGCGTTGCGGTCAACTTCTTGGTGCCCATGATGGTCACGGCGTCGACTGGCCGTTTCGGCGCCGTC
GCCCAGCGTGAACTGAGGGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MGKGSMTAHATPNEPDYPPPPGGPPPPADIGRLLLRCHDRPGIIAAVSTFLARAGANIISLDQHSTAPEGGTFLQRAIFH
LPGLTAAVDELQRDFGSTVADKFGIDYRFAEAAKPKRVAIMASTEDHCLLDLLWRNRRGELEMSVVMVIANHPDLAAHVR
PFGVPFIHIPATRDTRTEAEQRQLQLLSGNVDLVVLARYMQILSPGFLEAIGCPLINIHHSFLPAFTGAAPYQRARERGV
KLIGATAHYVTEVLDEGPIIEQDVVRVDHTHTVDDLVRVGADVERAVLSRAVLWHCQDRVIVHHNQTIVF

Sequences:

>Translated_310_residues
MGKGSMTAHATPNEPDYPPPPGGPPPPADIGRLLLRCHDRPGIIAAVSTFLARAGANIISLDQHSTAPEGGTFLQRAIFH
LPGLTAAVDELQRDFGSTVADKFGIDYRFAEAAKPKRVAIMASTEDHCLLDLLWRNRRGELEMSVVMVIANHPDLAAHVR
PFGVPFIHIPATRDTRTEAEQRQLQLLSGNVDLVVLARYMQILSPGFLEAIGCPLINIHHSFLPAFTGAAPYQRARERGV
KLIGATAHYVTEVLDEGPIIEQDVVRVDHTHTVDDLVRVGADVERAVLSRAVLWHCQDRVIVHHNQTIVF
>Mature_309_residues
GKGSMTAHATPNEPDYPPPPGGPPPPADIGRLLLRCHDRPGIIAAVSTFLARAGANIISLDQHSTAPEGGTFLQRAIFHL
PGLTAAVDELQRDFGSTVADKFGIDYRFAEAAKPKRVAIMASTEDHCLLDLLWRNRRGELEMSVVMVIANHPDLAAHVRP
FGVPFIHIPATRDTRTEAEQRQLQLLSGNVDLVVLARYMQILSPGFLEAIGCPLINIHHSFLPAFTGAAPYQRARERGVK
LIGATAHYVTEVLDEGPIIEQDVVRVDHTHTVDDLVRVGADVERAVLSRAVLWHCQDRVIVHHNQTIVF

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI209869995, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI209869993, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1787483, Length=287, Percent_Identity=40.418118466899, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1788846, Length=114, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=169, Percent_Identity=34.9112426035503, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24582400, Length=158, Percent_Identity=33.5443037974684, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURU_MYCBO (P0A5T7)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856633.1
- ProteinModelPortal:   P0A5T7
- SMR:   P0A5T7
- EnsemblBacteria:   EBMYCT00000016204
- GeneID:   1093549
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2988
- GeneTree:   EBGT00050000015793
- HOGENOM:   HBG286016
- OMA:   HQLNIIK
- ProtClustDB:   PRK06027
- BioCyc:   MBOV233413:MB2988-MONOMER
- BRENDA:   3.5.1.10
- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810
- Gene3D:   G3DSA:3.40.50.170
- PIRSF:   PIRSF036480
- PRINTS:   PR01575
- TIGRFAMs:   TIGR00655

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N; SSF53328 formyl_transf

EC number: =3.5.1.10

Molecular weight: Translated: 34006; Mature: 33875

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: ACT_SITE 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKGSMTAHATPNEPDYPPPPGGPPPPADIGRLLLRCHDRPGIIAAVSTFLARAGANIIS
CCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEE
LDQHSTAPEGGTFLQRAIFHLPGLTAAVDELQRDFGSTVADKFGIDYRFAEAAKPKRVAI
ECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEE
MASTEDHCLLDLLWRNRRGELEMSVVMVIANHPDLAAHVRPFGVPFIHIPATRDTRTEAE
EECCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCEEEECCCCCCHHHHH
QRQLQLLSGNVDLVVLARYMQILSPGFLEAIGCPLINIHHSFLPAFTGAAPYQRARERGV
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHCCC
KLIGATAHYVTEVLDEGPIIEQDVVRVDHTHTVDDLVRVGADVERAVLSRAVLWHCQDRV
EEEEHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCE
IVHHNQTIVF
EEECCCEEEC
>Mature Secondary Structure 
GKGSMTAHATPNEPDYPPPPGGPPPPADIGRLLLRCHDRPGIIAAVSTFLARAGANIIS
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEE
LDQHSTAPEGGTFLQRAIFHLPGLTAAVDELQRDFGSTVADKFGIDYRFAEAAKPKRVAI
ECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEE
MASTEDHCLLDLLWRNRRGELEMSVVMVIANHPDLAAHVRPFGVPFIHIPATRDTRTEAE
EECCCCHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCEEEECCCCCCHHHHH
QRQLQLLSGNVDLVVLARYMQILSPGFLEAIGCPLINIHHSFLPAFTGAAPYQRARERGV
HHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHCCC
KLIGATAHYVTEVLDEGPIIEQDVVRVDHTHTVDDLVRVGADVERAVLSRAVLWHCQDRV
EEEEHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCE
IVHHNQTIVF
EEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972