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The map label for this gene is mutM

Identifier: 148824114

GI number: 148824114

Start: 3250074

End: 3250943

Strand: Reverse

Name: mutM

Synonym: TBFG_12939

Alternate gene names: 148824114

Gene position: 3250943-3250074 (Counterclockwise)

Preceding gene: 148824115

Following gene: 148824113

Centisome position: 73.48

GC content: 67.13

Gene sequence:

>870_bases
ATGCCCGAGCTGCCCGAAGTCGAGGTGGTGCGGCGCGGCTTGCAGGCTCACGTGACCGGCCGGACCATCACCGAGGTTCG
GGTGCACCACCCCCGCGCTGTGCGCCGCCACGATGCCGGGCCCGCGGATCTGACGGCGCGGCTGCGGGGAGCGCGGATCA
ACGGAACCGATCGGCGCGGCAAGTACCTGTGGTTGACACTCAATACGGCTGGGGTCCATAGGCCGACGGACACCGCACTC
GTGGTGCACCTGGGCATGAGTGGGCAGATGCTGCTCGGGGCGGTGCCGTGTGCCGCTCACGTCCGGATTTCCGCGCTGCT
CGACGACGGGACCGTGCTGAGCTTCGCTGACCAACGGACCTTCGGAGGGTGGCTGCTTGCCGACCTGGTGACGGTGGACG
GCAGCGTGGTACCGGTGCCGGTCGCCCACCTGGCGCGCGACCCGCTTGACCCGCGGTTCGATTGTGACGCTGTAGTTAAA
GTGTTGCGGCGCAAGCATTCCGAACTCAAGCGCCAGCTGCTGGATCAGCGGGTGGTGTCGGGAATCGGCAACATCTATGC
CGATGAGGCGCTGTGGCGGGCCAAGGTGAACGGCGCCCACGTCGCCGCCACACTAAGGTGCCGGCGTCTGGGAGCGGTCC
TGCATGCCGCCGCCGACGTGATGCGCGAAGCGCTGGCGAAAGGTGGCACCTCGTTCGACTCTTTGTATGTCAACGTCAAC
GGCGAGTCGGGCTACTTCGAGCGGTCGCTGGACGCTTATGGCCGCGAAGGCGAAAACTGTCGGCGCTGCGGCGCGGTGAT
ACGCCGGGAGAGGTTTATGAACCGCTCGTCGTTCTACTGCCCGCGATGCCAGCCGCGGCCCCGAAAGTAG

Upstream 100 bases:

>100_bases
ATGACCCGCTGCGCCCGGCTTCGCCGCGCTTGCGATCATCACTGGTTCGATGGTGATGACCCGCTGCGCCCGGCTTCGCC
GCGCTTGCGATCATCACTGG

Downstream 100 bases:

>100_bases
GGCGGGTGTTGACGCATCGAGATCGACGTCAGCGCGATGCTCTCTCTCGCGGGGGCGCTGCCGTCGATCTCGACGCAAAG
ACCCCCGGGTAGAAAGAAGC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPELPEVEVVRRGLQAHVTGRTITEVRVHHPRAVRRHDAGPADLTARLRGARINGTDRRGKYLWLTLNTAGVHRPTDTAL
VVHLGMSGQMLLGAVPCAAHVRISALLDDGTVLSFADQRTFGGWLLADLVTVDGSVVPVPVAHLARDPLDPRFDCDAVVK
VLRRKHSELKRQLLDQRVVSGIGNIYADEALWRAKVNGAHVAATLRCRRLGAVLHAAADVMREALAKGGTSFDSLYVNVN
GESGYFERSLDAYGREGENCRRCGAVIRRERFMNRSSFYCPRCQPRPRK

Sequences:

>Translated_289_residues
MPELPEVEVVRRGLQAHVTGRTITEVRVHHPRAVRRHDAGPADLTARLRGARINGTDRRGKYLWLTLNTAGVHRPTDTAL
VVHLGMSGQMLLGAVPCAAHVRISALLDDGTVLSFADQRTFGGWLLADLVTVDGSVVPVPVAHLARDPLDPRFDCDAVVK
VLRRKHSELKRQLLDQRVVSGIGNIYADEALWRAKVNGAHVAATLRCRRLGAVLHAAADVMREALAKGGTSFDSLYVNVN
GESGYFERSLDAYGREGENCRRCGAVIRRERFMNRSSFYCPRCQPRPRK
>Mature_288_residues
PELPEVEVVRRGLQAHVTGRTITEVRVHHPRAVRRHDAGPADLTARLRGARINGTDRRGKYLWLTLNTAGVHRPTDTALV
VHLGMSGQMLLGAVPCAAHVRISALLDDGTVLSFADQRTFGGWLLADLVTVDGSVVPVPVAHLARDPLDPRFDCDAVVKV
LRRKHSELKRQLLDQRVVSGIGNIYADEALWRAKVNGAHVAATLRCRRLGAVLHAAADVMREALAKGGTSFDSLYVNVNG
ESGYFERSLDAYGREGENCRRCGAVIRRERFMNRSSFYCPRCQPRPRK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=289, Percent_Identity=33.2179930795848, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1786932, Length=291, Percent_Identity=25.7731958762887, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_MYCBO (P64151)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856594.1
- ProteinModelPortal:   P64151
- SMR:   P64151
- EnsemblBacteria:   EBMYCT00000017652
- GeneID:   1092157
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2949c
- GeneTree:   EBGT00050000016359
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- ProtClustDB:   PRK01103
- BioCyc:   MBOV233413:MB2949C-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31951; Mature: 31820

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 61-61 ACT_SITE 275-275 BINDING 100-100 BINDING 119-119 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVVRRGLQAHVTGRTITEVRVHHPRAVRRHDAGPADLTARLRGARINGTDRRG
CCCCCHHHHHHCCHHHEECCCEEEEEEECCCHHHHHCCCCCHHHHHHHCCCEECCCCCCC
KYLWLTLNTAGVHRPTDTALVVHLGMSGQMLLGAVPCAAHVRISALLDDGTVLSFADQRT
CEEEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCHHHHEEEEEEECCCCEEEECCCCC
FGGWLLADLVTVDGSVVPVPVAHLARDPLDPRFDCDAVVKVLRRKHSELKRQLLDQRVVS
CCHHHHHHHHHCCCCEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GIGNIYADEALWRAKVNGAHVAATLRCRRLGAVLHAAADVMREALAKGGTSFDSLYVNVN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
GESGYFERSLDAYGREGENCRRCGAVIRRERFMNRSSFYCPRCQPRPRK
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVEVVRRGLQAHVTGRTITEVRVHHPRAVRRHDAGPADLTARLRGARINGTDRRG
CCCCHHHHHHCCHHHEECCCEEEEEEECCCHHHHHCCCCCHHHHHHHCCCEECCCCCCC
KYLWLTLNTAGVHRPTDTALVVHLGMSGQMLLGAVPCAAHVRISALLDDGTVLSFADQRT
CEEEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCHHHHEEEEEEECCCCEEEECCCCC
FGGWLLADLVTVDGSVVPVPVAHLARDPLDPRFDCDAVVKVLRRKHSELKRQLLDQRVVS
CCHHHHHHHHHCCCCEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GIGNIYADEALWRAKVNGAHVAATLRCRRLGAVLHAAADVMREALAKGGTSFDSLYVNVN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
GESGYFERSLDAYGREGENCRRCGAVIRRERFMNRSSFYCPRCQPRPRK
CCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972