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The map label for this gene is trmD

Identifier: 148824095

GI number: 148824095

Start: 3227194

End: 3227886

Strand: Reverse

Name: trmD

Synonym: TBFG_12920

Alternate gene names: 148824095

Gene position: 3227886-3227194 (Counterclockwise)

Preceding gene: 148824096

Following gene: 148824093

Centisome position: 72.96

GC content: 65.8

Gene sequence:

>693_bases
GTGCGCATCGATATCGTGACGATCTTCCCCGCCTGCCTGGACCCGTTGCGACAGTCGTTGCCGGGCAAGGCGATCGAATC
GGGCCTGGTCGACCTTAACGTGCACGACCTGCGGCGCTGGACCCACGATGTGCATCACTCGGTGGACGACGCACCCTACG
GCGGCGGCCCGGGAATGGTGATGAAGGCGCCGGTGTGGGGTGAAGCGCTTGACGAAATTTGTTCCAGCGAAACGTTGTTG
ATTGTTCCCACGCCCGCCGGTGTGTTGTTTACCCAGGCCACCGCCCAGCGCTGGACCACCGAGAGCCATCTGGTGTTCGC
CTGCGGCCGCTACGAAGGCATCGACCAGCGGGTCGTGCAGGATGCCGCCCGGCGAATGCGCGTCGAAGAAGTCTCGATCG
GCGACTACGTATTGCCCGGCGGGGAGTCGGCGGCCGTGGTGATGGTCGAAGCCGTGCTGCGACTGTTGGCCGGTGTTCTC
GGCAATCCCGCTTCCCACCAAGATGATTCGCACTCAACGGGCCTGGACGGGCTCCTTGAGGGTCCGAGCTACACCCGGCC
GGCGAGCTGGCGTGGCCTCGATGTACCCGAGGTCCTACTCTCCGGCGACCATGCCCGAATTGCCGCCTGGCGCAGAGAGG
TCTCGCTGCAGCGCACCCGCGAACGACGCCCCGACCTGTCCCACCCCGACTAG

Upstream 100 bases:

>100_bases
TTTGGTGCCGTTTGTGCGTGCCATCGTCACGTCGGTGTCATTGGACGACGGCATCGTTGAGATTGATCCGCCCCATGGCT
TGCTGAATCTGGAGTAAGGC

Downstream 100 bases:

>100_bases
CCCCGCTGGCGAGCAGACGCGAAATCCCCCGACACGCCGAACGTGCGGTGGCTTTTGCGTCTGCTCGCGCCGGTGAGGCG
ACCGGACGAGCGTCAGATCC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRIDIVTIFPACLDPLRQSLPGKAIESGLVDLNVHDLRRWTHDVHHSVDDAPYGGGPGMVMKAPVWGEALDEICSSETLL
IVPTPAGVLFTQATAQRWTTESHLVFACGRYEGIDQRVVQDAARRMRVEEVSIGDYVLPGGESAAVVMVEAVLRLLAGVL
GNPASHQDDSHSTGLDGLLEGPSYTRPASWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPDLSHPD

Sequences:

>Translated_230_residues
MRIDIVTIFPACLDPLRQSLPGKAIESGLVDLNVHDLRRWTHDVHHSVDDAPYGGGPGMVMKAPVWGEALDEICSSETLL
IVPTPAGVLFTQATAQRWTTESHLVFACGRYEGIDQRVVQDAARRMRVEEVSIGDYVLPGGESAAVVMVEAVLRLLAGVL
GNPASHQDDSHSTGLDGLLEGPSYTRPASWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPDLSHPD
>Mature_230_residues
MRIDIVTIFPACLDPLRQSLPGKAIESGLVDLNVHDLRRWTHDVHHSVDDAPYGGGPGMVMKAPVWGEALDEICSSETLL
IVPTPAGVLFTQATAQRWTTESHLVFACGRYEGIDQRVVQDAARRMRVEEVSIGDYVLPGGESAAVVMVEAVLRLLAGVL
GNPASHQDDSHSTGLDGLLEGPSYTRPASWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPDLSHPD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=230, Percent_Identity=42.1739130434783, Blast_Score=163, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_MYCBO (P66969)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856575.1
- ProteinModelPortal:   P66969
- SMR:   P66969
- EnsemblBacteria:   EBMYCT00000017809
- GeneID:   1092177
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2930c
- GeneTree:   EBGT00050000016420
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   MBOV233413:MB2930C-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25167; Mature: 25167

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDIVTIFPACLDPLRQSLPGKAIESGLVDLNVHDLRRWTHDVHHSVDDAPYGGGPGMV
CEEEEEEHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKAPVWGEALDEICSSETLLIVPTPAGVLFTQATAQRWTTESHLVFACGRYEGIDQRVVQ
EECCCCHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHCCCCCCEEEEECCCCCHHHHHHH
DAARRMRVEEVSIGDYVLPGGESAAVVMVEAVLRLLAGVLGNPASHQDDSHSTGLDGLLE
HHHHHHHHHEECCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCC
GPSYTRPASWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPDLSHPD
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIDIVTIFPACLDPLRQSLPGKAIESGLVDLNVHDLRRWTHDVHHSVDDAPYGGGPGMV
CEEEEEEHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCE
MKAPVWGEALDEICSSETLLIVPTPAGVLFTQATAQRWTTESHLVFACGRYEGIDQRVVQ
EECCCCHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHCCCCCCEEEEECCCCCHHHHHHH
DAARRMRVEEVSIGDYVLPGGESAAVVMVEAVLRLLAGVLGNPASHQDDSHSTGLDGLLE
HHHHHHHHHEECCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCC
GPSYTRPASWRGLDVPEVLLSGDHARIAAWRREVSLQRTRERRPDLSHPD
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972