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The map label for this gene is 148824040

Identifier: 148824040

GI number: 148824040

Start: 3169803

End: 3171692

Strand: Reverse

Name: 148824040

Synonym: TBFG_12865

Alternate gene names: NA

Gene position: 3171692-3169803 (Counterclockwise)

Preceding gene: 148824041

Following gene: 148824039

Centisome position: 71.69

GC content: 70.48

Gene sequence:

>1890_bases
GTGAAGCCTTATCCGTTCAGCGCGATCGTCGGGCACGATCGGCTGCGGCTCGCGTTGTTGTTGTGTGCCGTGCGACCGGA
GATCGGTGGGGCGCTCATCCGTGGCGAGAAGGGCACGGCGAAATCGACGGCGGTGCGCGGGCTGGCCGCGTTGTTGTCGG
TCGCGACCGGGAGCACCGAGACCGGCCTCGTGGAGCTGCCGCTGGGGGCCACCGAAGACCGGGTGGTTGGCTCGCTGGAT
CTGCAGCGGGTGATGCGCGACGGCGAGCACGCGTTCTCTCCGGGACTGCTGGCTCGCGCCCACGGCGGCGTGCTCTACGT
CGACGAGGTCAATCTGCTGCATGATCACCTGGTTGACATCCTGCTCGACGCCGCCGCCATGGGACGCGTACACGTCGAAC
GCGACGGCATCTCCCATTCCCACGAGGCCCGTTTCGTGCTGATCGGCACGATGAATCCGGAGGAGGGCGAACTGCGTCCG
CAGCTGCTGGACCGGTTCGGGCTGACCGTCGACGTGCAGGCGTCACGTGACATCGACGTGCGGGTACAGGTCATCCGCCG
GCGGATGGCCTACGAAGCCGACCCGGATGCGTTCGTCGCACGCTATGCCGACGCCGACGCGGAGCTGGCCCACCGGATTG
CCGCGGCCCGGGCCACGGTAGACGATGTGGTGTTGGGCGACAACGAGTTGCGGCGCATCGCTGCGTTGTGCGCGGCGTTC
GACGTGGACGGCATGCGGGCCGATCTGGTAGTGGCCCGGACCGCCGCCGCGCACGCCGCCTGGCGTGGTGTCCGCACCGT
TGAGGAGCAGGATATCCGGGCGGCAGCAGAACTGGCGTTGCCGCATCGTCGTCGTCGCGACCCGTTCGACGATCACGGCA
TCGACCGCGACCAGCTGGATGAGGCGCTGGCGCTGGCGAGCGTCGACCCAGAGCCCGAGCCCGATCCGCCCGGGGGCGGC
CAGTCAGCCAATGAACCTGCCTCACAACCGAACTCACGCTCGAAGTCGACAGAACCTGGTGCGCCGAGCTCGATGGGCGA
CGACCCGCCGCGCCCGGCTTCGCCGCGCTTGCGATCGTCGCCGCGCCCGAGCGCGCCGCCGTCAAAGATATTCCGGACCC
GCGCGCTGCGGGTCCCGGGGGTCGGCACGGGCGCGCCGGGGCGACGGTCTCGGGCCCGCAACGCCTCGGGCAGTGTGGTG
GCGGCCGCCGAGGTCAGCGACCCCGACGCACATGGACTGCACCTGTTCGCTACCCTGCTGGCCGCCGGCGAGCGTGCGTT
CGGAGCCGGGCCGTTGCGCCCGTGGCCGGACGATGTGCGCCGGGCCATCCGCGAGGGTCGCGAAGGCAATCTGGTGATCT
TCGTCGTCGACGCTTCCGGATCGATGGCTGCTCGGGACCGGATGGCTGCGGTCAGTGGCGCCACCCTGTCGCTGCTGCGC
GACGCCTACCAGCGCAGGGACAAGGTGGCGGTGATCACCTTCCGCCAGCACGAGGCAACGCTGTTGCTGTCGCCGACGTC
GTCGGCGCACATCGCCGGCCGGCGACTGGCCCGATTCAGCACCGGCGGCAAGACCCCGCTGGCTGAGGGACTGCTGGCCG
CGCGTGCGCTGATCATCCGGGAGAAGGTACGCGACCGCGCCCGACGCCCCCTGGTGGTCGTGCTGACCGACGGCCGGGCC
ACCGCCGGGCCGGACCCGTTGGGCCGCAGCCGAACCGCGGCTGCCGGGCTGGTCGCCGAGGGCGCGGCCGCGGTGGTGGT
GGACTGCGAAACGTCGTATGTACGGCTGGGTTTGGCAGCGCAACTGGCTCGTCAGCTGGGTGCGCCGGTGGTTCGGTTGG
AGCAGTTGCACGCGGATTATCTAGTGCATGCCGTGCGTGGCGTGGCTTGA

Upstream 100 bases:

>100_bases
ATGCCCAGCACGGCTTCGTCCGCGACGGTGACGAGTTCCTGGACGACGGCATCCCGCATGTGCCCATGTTGCGGCCCGGC
TCCGGTCAGGTGGAGCGGCC

Downstream 100 bases:

>100_bases
CGATGGATAGGTAACCACACATGCCGCAGGGCAATCCGCTCGCAGTGCCCAACGATGGCCTGACCACCCGGGCCCGGCGC
AACATGCCGATACTGGCGGT

Product: magnesium chelatase

Products: ADP; phosphate; Mg-protoporphyrin IX; H+

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MKPYPFSAIVGHDRLRLALLLCAVRPEIGGALIRGEKGTAKSTAVRGLAALLSVATGSTETGLVELPLGATEDRVVGSLD
LQRVMRDGEHAFSPGLLARAHGGVLYVDEVNLLHDHLVDILLDAAAMGRVHVERDGISHSHEARFVLIGTMNPEEGELRP
QLLDRFGLTVDVQASRDIDVRVQVIRRRMAYEADPDAFVARYADADAELAHRIAAARATVDDVVLGDNELRRIAALCAAF
DVDGMRADLVVARTAAAHAAWRGVRTVEEQDIRAAAELALPHRRRRDPFDDHGIDRDQLDEALALASVDPEPEPDPPGGG
QSANEPASQPNSRSKSTEPGAPSSMGDDPPRPASPRLRSSPRPSAPPSKIFRTRALRVPGVGTGAPGRRSRARNASGSVV
AAAEVSDPDAHGLHLFATLLAAGERAFGAGPLRPWPDDVRRAIREGREGNLVIFVVDASGSMAARDRMAAVSGATLSLLR
DAYQRRDKVAVITFRQHEATLLLSPTSSAHIAGRRLARFSTGGKTPLAEGLLAARALIIREKVRDRARRPLVVVLTDGRA
TAGPDPLGRSRTAAAGLVAEGAAAVVVDCETSYVRLGLAAQLARQLGAPVVRLEQLHADYLVHAVRGVA

Sequences:

>Translated_629_residues
MKPYPFSAIVGHDRLRLALLLCAVRPEIGGALIRGEKGTAKSTAVRGLAALLSVATGSTETGLVELPLGATEDRVVGSLD
LQRVMRDGEHAFSPGLLARAHGGVLYVDEVNLLHDHLVDILLDAAAMGRVHVERDGISHSHEARFVLIGTMNPEEGELRP
QLLDRFGLTVDVQASRDIDVRVQVIRRRMAYEADPDAFVARYADADAELAHRIAAARATVDDVVLGDNELRRIAALCAAF
DVDGMRADLVVARTAAAHAAWRGVRTVEEQDIRAAAELALPHRRRRDPFDDHGIDRDQLDEALALASVDPEPEPDPPGGG
QSANEPASQPNSRSKSTEPGAPSSMGDDPPRPASPRLRSSPRPSAPPSKIFRTRALRVPGVGTGAPGRRSRARNASGSVV
AAAEVSDPDAHGLHLFATLLAAGERAFGAGPLRPWPDDVRRAIREGREGNLVIFVVDASGSMAARDRMAAVSGATLSLLR
DAYQRRDKVAVITFRQHEATLLLSPTSSAHIAGRRLARFSTGGKTPLAEGLLAARALIIREKVRDRARRPLVVVLTDGRA
TAGPDPLGRSRTAAAGLVAEGAAAVVVDCETSYVRLGLAAQLARQLGAPVVRLEQLHADYLVHAVRGVA
>Mature_629_residues
MKPYPFSAIVGHDRLRLALLLCAVRPEIGGALIRGEKGTAKSTAVRGLAALLSVATGSTETGLVELPLGATEDRVVGSLD
LQRVMRDGEHAFSPGLLARAHGGVLYVDEVNLLHDHLVDILLDAAAMGRVHVERDGISHSHEARFVLIGTMNPEEGELRP
QLLDRFGLTVDVQASRDIDVRVQVIRRRMAYEADPDAFVARYADADAELAHRIAAARATVDDVVLGDNELRRIAALCAAF
DVDGMRADLVVARTAAAHAAWRGVRTVEEQDIRAAAELALPHRRRRDPFDDHGIDRDQLDEALALASVDPEPEPDPPGGG
QSANEPASQPNSRSKSTEPGAPSSMGDDPPRPASPRLRSSPRPSAPPSKIFRTRALRVPGVGTGAPGRRSRARNASGSVV
AAAEVSDPDAHGLHLFATLLAAGERAFGAGPLRPWPDDVRRAIREGREGNLVIFVVDASGSMAARDRMAAVSGATLSLLR
DAYQRRDKVAVITFRQHEATLLLSPTSSAHIAGRRLARFSTGGKTPLAEGLLAARALIIREKVRDRARRPLVVVLTDGRA
TAGPDPLGRSRTAAAGLVAEGAAAVVVDCETSYVRLGLAAQLARQLGAPVVRLEQLHADYLVHAVRGVA

Specific function: Unknown

COG id: COG1239

COG function: function code H; Mg-chelatase subunit ChlI

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2850_MYCTU (O05809)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   E70589
- RefSeq:   NP_217366.1
- RefSeq:   NP_337428.1
- ProteinModelPortal:   O05809
- SMR:   O05809
- EnsemblBacteria:   EBMYCT00000002250
- EnsemblBacteria:   EBMYCT00000070084
- GeneID:   887364
- GeneID:   925362
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2916
- KEGG:   mtu:Rv2850c
- TIGR:   MT2916
- TubercuList:   Rv2850c
- GeneTree:   EBGT00050000015565
- HOGENOM:   HBG553490
- OMA:   LPTGGRT
- ProtClustDB:   CLSK792122
- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR012804
- InterPro:   IPR002035
- SMART:   SM00382
- SMART:   SM00327
- TIGRFAMs:   TIGR02442

Pfam domain/function: PF07728 AAA_5; PF00092 VWA

EC number: 6.6.1.1

Molecular weight: Translated: 66988; Mature: 66988

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50234 VWFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYPFSAIVGHDRLRLALLLCAVRPEIGGALIRGEKGTAKSTAVRGLAALLSVATGSTE
CCCCCCHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
TGLVELPLGATEDRVVGSLDLQRVMRDGEHAFSPGLLARAHGGVLYVDEVNLLHDHLVDI
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHH
LLDAAAMGRVHVERDGISHSHEARFVLIGTMNPEEGELRPQLLDRFGLTVDVQASRDIDV
HHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCEEEEECCCCCHH
RVQVIRRRMAYEADPDAFVARYADADAELAHRIAAARATVDDVVLGDNELRRIAALCAAF
HHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHH
DVDGMRADLVVARTAAAHAAWRGVRTVEEQDIRAAAELALPHRRRRDPFDDHGIDRDQLD
CCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHH
EALALASVDPEPEPDPPGGGQSANEPASQPNSRSKSTEPGAPSSMGDDPPRPASPRLRSS
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRPSAPPSKIFRTRALRVPGVGTGAPGRRSRARNASGSVVAAAEVSDPDAHGLHLFATLL
CCCCCCHHHHHHHHHEECCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
AAGERAFGAGPLRPWPDDVRRAIREGREGNLVIFVVDASGSMAARDRMAAVSGATLSLLR
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHH
DAYQRRDKVAVITFRQHEATLLLSPTSSAHIAGRRLARFSTGGKTPLAEGLLAARALIIR
HHHHCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EKVRDRARRPLVVVLTDGRATAGPDPLGRSRTAAAGLVAEGAAAVVVDCETSYVRLGLAA
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
QLARQLGAPVVRLEQLHADYLVHAVRGVA
HHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPYPFSAIVGHDRLRLALLLCAVRPEIGGALIRGEKGTAKSTAVRGLAALLSVATGSTE
CCCCCCHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
TGLVELPLGATEDRVVGSLDLQRVMRDGEHAFSPGLLARAHGGVLYVDEVNLLHDHLVDI
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHH
LLDAAAMGRVHVERDGISHSHEARFVLIGTMNPEEGELRPQLLDRFGLTVDVQASRDIDV
HHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCEEEEECCCCCHH
RVQVIRRRMAYEADPDAFVARYADADAELAHRIAAARATVDDVVLGDNELRRIAALCAAF
HHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHH
DVDGMRADLVVARTAAAHAAWRGVRTVEEQDIRAAAELALPHRRRRDPFDDHGIDRDQLD
CCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHH
EALALASVDPEPEPDPPGGGQSANEPASQPNSRSKSTEPGAPSSMGDDPPRPASPRLRSS
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PRPSAPPSKIFRTRALRVPGVGTGAPGRRSRARNASGSVVAAAEVSDPDAHGLHLFATLL
CCCCCCHHHHHHHHHEECCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
AAGERAFGAGPLRPWPDDVRRAIREGREGNLVIFVVDASGSMAARDRMAAVSGATLSLLR
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHH
DAYQRRDKVAVITFRQHEATLLLSPTSSAHIAGRRLARFSTGGKTPLAEGLLAARALIIR
HHHHCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EKVRDRARRPLVVVLTDGRATAGPDPLGRSRTAAAGLVAEGAAAVVVDCETSYVRLGLAA
HHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
QLARQLGAPVVRLEQLHADYLVHAVRGVA
HHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; protoporphyrin IX; Mg2+; H2O

Specific reaction: ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036