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The map label for this gene is cocE [H]

Identifier: 148823988

GI number: 148823988

Start: 3120371

End: 3122020

Strand: Direct

Name: cocE [H]

Synonym: TBFG_12813

Alternate gene names: 148823988

Gene position: 3120371-3122020 (Clockwise)

Preceding gene: 148823987

Following gene: 148823991

Centisome position: 70.53

GC content: 70.91

Gene sequence:

>1650_bases
GTGAGTACCACCTCCGCTCGGCCCGAGCGGCCCAAGCTGCGCGCCCTGACCGGACGAGTCGGTGGGCAGGCCCTGGGCGG
ACTGTTGGGTCTGCCCCGCGCAACCACCCGCTACACCGTCGGTCACGTCCGAGTCCCGATGCGCGACGGCGTCCAGCTGG
TGGCCGACCACTACGCACCCGCCACGTCGCAGCCCGTCGGCACCCTGCTGGTGCGTGGGCCATACGGGCGCCGGTTTCCG
TTTTCGCTGGTGTTTGCCCGGATTTACGCCGCCCGCGGTTATCACGTCGTGCTGCAGAGCGTGCGCGGGACGTTCGGGTC
CGGTGGCGTGTTCGAGCCCATGGTCAACGAGGCCGCCGACGGCGCCGATACGGTGGCGTGGCTGCGTGAACAGCCCTGGT
TCACCGGCCGGTTCGGCACCATCGGCCTGCCCTATCTGGGTTTCACCCAGTGGGCGTTGCTGCACGATCCGCCCCCGGAG
CTGGCCGCGGCCGTGATCACGGTGGGGCCGCACGACTTCCGGGCCTCGGTGTGGGGCACCGGATCGTTTACGGTCAACGA
CTTCCTGGGCTGGAGCGATCTGGTTTCCCACCAGGAAGACCCCGGTCGCATCCGGGCCGGAATCCGCCAGCTCACCGCGC
CGCGACGGGTGGCGCGGACGGCCGCCACGTTGCCGCTGGGTGAGTCGGCCCGGACGCTGCTCGGCACGGGTGCGCCGTGG
TTCGAATCCTGGGTGGAACACACCGACCGCGACGATCCGTTCTGGGACCGACTGCGGTTTCCCGCCGCGTTGGACCGCGT
CCAGGTCCCGGTGCTGCTCGTCGGCGGCTGGCAGGACATCTTCCTGCGGCAGACGCTGCAGCAGTACCGGCACCTGCGCG
ACCGGGGTGTGCACGTCGCGCTGACGGTCGGTCCCTGGACACACACCCAGATGCTCACCAAGGGGCTGGCCACCGGCGCT
CGGGAATCGTTGGACTGGTTGGACGCCCACCTCGGCCGGGCGCCGGCGCTGCGCCCCAGCCCGGTGCGGGTCTTCGTCAC
CGGCCAGGGCTGGCGGCACCTGCCGGACTGGCCTCCGGCGACCACCGAGCGGGCGTGGTACCTGCAGCCCGGTGGCCGCC
TGGGTGAGAGCGCTCCGGCTTCCGGCACGCCACCGGCGACGTTTCGCTACCACCCCGCCGACCCGACACCGACCACCGGT
GGTCCGCTACTGTCATCCAACGGCGGTTACCGCGACGACAGCCGGCTGGCCACGCGCGCCGATGTGCTGTGCTTCACCGG
GGCGCCCCTCACCCACGACCTCTGCGTGCACGGAAACCCCGTCGTCGAGCTGGTGCACAGCTCGGACAACCCCTACGTCG
ACGTGTTCGTTCGGGTCAGCGAGGTGGACGCGAAGGGCCGGTCCCGCAATGTCAGCGACGGCTACCGGCGCCTTGGTGAC
GCGCCGGAGCTGGTCCGCGTCGAGCTGGACGCCATCGCCCACCGATTCCGCGCCGACTCCCGCATCCGGGTGCTGATCGC
CGGTAGTTGGTTTCCCCGCTATGCGCGAAACCTCGGCACCCCGGAACCGATACTCACCGGACGGCAGCTCAAGCCGGCTA
CCCACGCGGTGCATTTCGGGCGCTCCCGGCTGCTGCTGCCCGTCGGCTAA

Upstream 100 bases:

>100_bases
CGTCTACTCCGAGCCCGACGTGGACTGCCCGTCGACCAACCTGCAGCGGGCAAACGACCTCGTCCCCTACTACAGGTTTT
AGGTCCCTACCCTGGGCGTC

Downstream 100 bases:

>100_bases
CGGCTGGTGGTGCGGCGGACCCGGGCGGCGACCCGGCCGATAACCCGAGCCCGTCCAGCGGCGCGTGCCCAGTGGTCTCC
CCGACGCGGAACCTGCGAGA

Product: hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSTTSARPERPKLRALTGRVGGQALGGLLGLPRATTRYTVGHVRVPMRDGVQLVADHYAPATSQPVGTLLVRGPYGRRFP
FSLVFARIYAARGYHVVLQSVRGTFGSGGVFEPMVNEAADGADTVAWLREQPWFTGRFGTIGLPYLGFTQWALLHDPPPE
LAAAVITVGPHDFRASVWGTGSFTVNDFLGWSDLVSHQEDPGRIRAGIRQLTAPRRVARTAATLPLGESARTLLGTGAPW
FESWVEHTDRDDPFWDRLRFPAALDRVQVPVLLVGGWQDIFLRQTLQQYRHLRDRGVHVALTVGPWTHTQMLTKGLATGA
RESLDWLDAHLGRAPALRPSPVRVFVTGQGWRHLPDWPPATTERAWYLQPGGRLGESAPASGTPPATFRYHPADPTPTTG
GPLLSSNGGYRDDSRLATRADVLCFTGAPLTHDLCVHGNPVVELVHSSDNPYVDVFVRVSEVDAKGRSRNVSDGYRRLGD
APELVRVELDAIAHRFRADSRIRVLIAGSWFPRYARNLGTPEPILTGRQLKPATHAVHFGRSRLLLPVG

Sequences:

>Translated_549_residues
MSTTSARPERPKLRALTGRVGGQALGGLLGLPRATTRYTVGHVRVPMRDGVQLVADHYAPATSQPVGTLLVRGPYGRRFP
FSLVFARIYAARGYHVVLQSVRGTFGSGGVFEPMVNEAADGADTVAWLREQPWFTGRFGTIGLPYLGFTQWALLHDPPPE
LAAAVITVGPHDFRASVWGTGSFTVNDFLGWSDLVSHQEDPGRIRAGIRQLTAPRRVARTAATLPLGESARTLLGTGAPW
FESWVEHTDRDDPFWDRLRFPAALDRVQVPVLLVGGWQDIFLRQTLQQYRHLRDRGVHVALTVGPWTHTQMLTKGLATGA
RESLDWLDAHLGRAPALRPSPVRVFVTGQGWRHLPDWPPATTERAWYLQPGGRLGESAPASGTPPATFRYHPADPTPTTG
GPLLSSNGGYRDDSRLATRADVLCFTGAPLTHDLCVHGNPVVELVHSSDNPYVDVFVRVSEVDAKGRSRNVSDGYRRLGD
APELVRVELDAIAHRFRADSRIRVLIAGSWFPRYARNLGTPEPILTGRQLKPATHAVHFGRSRLLLPVG
>Mature_548_residues
STTSARPERPKLRALTGRVGGQALGGLLGLPRATTRYTVGHVRVPMRDGVQLVADHYAPATSQPVGTLLVRGPYGRRFPF
SLVFARIYAARGYHVVLQSVRGTFGSGGVFEPMVNEAADGADTVAWLREQPWFTGRFGTIGLPYLGFTQWALLHDPPPEL
AAAVITVGPHDFRASVWGTGSFTVNDFLGWSDLVSHQEDPGRIRAGIRQLTAPRRVARTAATLPLGESARTLLGTGAPWF
ESWVEHTDRDDPFWDRLRFPAALDRVQVPVLLVGGWQDIFLRQTLQQYRHLRDRGVHVALTVGPWTHTQMLTKGLATGAR
ESLDWLDAHLGRAPALRPSPVRVFVTGQGWRHLPDWPPATTERAWYLQPGGRLGESAPASGTPPATFRYHPADPTPTTGG
PLLSSNGGYRDDSRLATRADVLCFTGAPLTHDLCVHGNPVVELVHSSDNPYVDVFVRVSEVDAKGRSRNVSDGYRRLGDA
PELVRVELDAIAHRFRADSRIRVLIAGSWFPRYARNLGTPEPILTGRQLKPATHAVHFGRSRLLLPVG

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 60285; Mature: 60154

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTSARPERPKLRALTGRVGGQALGGLLGLPRATTRYTVGHVRVPMRDGVQLVADHYAP
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEECHHCCHHHHHHHCCC
ATSQPVGTLLVRGPYGRRFPFSLVFARIYAARGYHVVLQSVRGTFGSGGVFEPMVNEAAD
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHCCC
GADTVAWLREQPWFTGRFGTIGLPYLGFTQWALLHDPPPELAAAVITVGPHDFRASVWGT
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHCEEEECCCCHHHHEEEEEECCCCCEEEEECC
GSFTVNDFLGWSDLVSHQEDPGRIRAGIRQLTAPRRVARTAATLPLGESARTLLGTGAPW
CCEEHHHHCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCCHH
FESWVEHTDRDDPFWDRLRFPAALDRVQVPVLLVGGWQDIFLRQTLQQYRHLRDRGVHVA
HHHHHHHCCCCCCHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEE
LTVGPWTHTQMLTKGLATGARESLDWLDAHLGRAPALRPSPVRVFVTGQGWRHLPDWPPA
EEECCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TTERAWYLQPGGRLGESAPASGTPPATFRYHPADPTPTTGGPLLSSNGGYRDDSRLATRA
CCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCCCHHHHHH
DVLCFTGAPLTHDLCVHGNPVVELVHSSDNPYVDVFVRVSEVDAKGRSRNVSDGYRRLGD
CEEEEECCCCCCCEEECCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHCC
APELVRVELDAIAHRFRADSRIRVLIAGSWFPRYARNLGTPEPILTGRQLKPATHAVHFG
CCHHEEEEHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
RSRLLLPVG
CCEEEEECC
>Mature Secondary Structure 
STTSARPERPKLRALTGRVGGQALGGLLGLPRATTRYTVGHVRVPMRDGVQLVADHYAP
CCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEECHHCCHHHHHHHCCC
ATSQPVGTLLVRGPYGRRFPFSLVFARIYAARGYHVVLQSVRGTFGSGGVFEPMVNEAAD
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHCCC
GADTVAWLREQPWFTGRFGTIGLPYLGFTQWALLHDPPPELAAAVITVGPHDFRASVWGT
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHCEEEECCCCHHHHEEEEEECCCCCEEEEECC
GSFTVNDFLGWSDLVSHQEDPGRIRAGIRQLTAPRRVARTAATLPLGESARTLLGTGAPW
CCEEHHHHCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCCHH
FESWVEHTDRDDPFWDRLRFPAALDRVQVPVLLVGGWQDIFLRQTLQQYRHLRDRGVHVA
HHHHHHHCCCCCCHHHHHCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCEEE
LTVGPWTHTQMLTKGLATGARESLDWLDAHLGRAPALRPSPVRVFVTGQGWRHLPDWPPA
EEECCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TTERAWYLQPGGRLGESAPASGTPPATFRYHPADPTPTTGGPLLSSNGGYRDDSRLATRA
CCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCCCCHHHHHH
DVLCFTGAPLTHDLCVHGNPVVELVHSSDNPYVDVFVRVSEVDAKGRSRNVSDGYRRLGD
CEEEEECCCCCCCEEECCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHCC
APELVRVELDAIAHRFRADSRIRVLIAGSWFPRYARNLGTPEPILTGRQLKPATHAVHFG
CCHHEEEEHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHCC
RSRLLLPVG
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]