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The map label for this gene is miaB

Identifier: 148823921

GI number: 148823921

Start: 3056941

End: 3058479

Strand: Reverse

Name: miaB

Synonym: TBFG_12746

Alternate gene names: 148823921

Gene position: 3058479-3056941 (Counterclockwise)

Preceding gene: 148823923

Following gene: 148823920

Centisome position: 69.13

GC content: 69.92

Gene sequence:

>1539_bases
ATGGTGGCGCACGATGCCGCCGCGGGGGTGACCGGCGAGGGCGCCGGGCCGCCCGTGCGGCGAGCGCCAGCGCGCACCTA
TCAGGTCCGCACCTACGGCTGTCAGATGAACGTCCACGACTCCGAGCGGCTGGCGGGTCTGCTGGAAGCGGCCGGCTACC
GGAGGGCGACCGACGGTTCCGAGGCCGACGTCGTGGTGTTCAACACCTGCGCCGTCCGCGAGAACGCCGACAACAGGCTG
TACGGCAACCTCAGCCATCTGGCCCCGCGCAAGCGCGCCAATCCCGACATGCAAATCGCGGTCGGTGGCTGCCTGGCGCA
AAAAGACCGCGACGCCGTGCTGCGCAGGGCGCCGTGGGTAGACGTTGTCTTCGGCACCCACAACATCGGGTCTTTGCCCA
CGCTGCTGGAGCGCGCCCGGCACAACAAGGTCGCCCAGGTCGAAATCGCCGAGGCGCTGCAACAGTTCCCGTCGTCGCTG
CCCAGCTCCCGCGAATCTGCCTATGCCGCGTGGGTTTCCATCTCGGTGGGCTGCAACAACAGCTGCACGTTCTGCATCGT
CCCGTCGCTGCGGGGTAGGGAGGTCGACCGCAGCCCGGCAGACATCCTGGCCGAGGTGCGGTCCTTGGTGAACGACGGTG
TGCTCGAAGTCACCCTGCTCGGCCAAAACGTCAACGCCTACGGCGTCTCGTTCGCCGACCCCGCATTGCCCCGTAATCGG
GGAGCTTTCGCCGAGTTGCTGCGGGCCTGCGGAGACATCGACGGGCTGGAGCGGGTCCGGTTCACCTCCCCACACCCGGC
CGAATTCACCGACGACGTCATCGAGGCGATGGCGCAGACGCGCAACGTCTGCCCCGCGCTGCACATGCCGCTACAGTCCG
GATCCGACCGGATCCTGCGCGCGATGCGGCGGTCCTATCGTGCCGAGCGCTACCTCGGCATCATCGAGCGCGTGCGGGCG
GCCATCCCGCATGCCGCGATTACCACCGATCTGATCGTCGGTTTCCCCGGGGAGACCGAAGAGGACTTCGCGGCCACGCT
CGACGTGGTGCGCCGGGCCCGCTTCGCGGCCGCATTTACCTTCCAATACTCCAAGCGGCCCGGGACACCGGCCGCGCAAC
TCGACGGACAGCTGCCGAAAGCCGTTGTGCAGGAACGCTATGAACGCCTGATCGCGCTGCAGGAGCAAATCTCGCTGGAA
GCCAACCGCGCCCTGGTCGGGCAGGCCGTCGAGGTGCTGGTGGCCACCGGGGAAGGGCGCAAGGACACCGTCACGGCGCG
CATGAGCGGGCGGGCGCGCGACGGGCGCCTGGTGCACTTCACCGCCGGCCAGCCGCGCGTGCGTCCCGGCGACGTCATCA
CCACGAAGGTCACCGAAGCGGCGCCGCACCACCTCATCGCCGACGCCGGCGTCCTGACCCACCGCCGCACCCGCGCCGGC
GACGCGCACACCGCCGGGCAGCCGGGGCGCGCCGTCGGCCTGGGCATGCCTGGGGTCGGGCTGCCGGTCAGCGCGGCCAA
GCCCGGAGGATGCAGATGA

Upstream 100 bases:

>100_bases
CGTGTCGAGCGCGTCCCCGCTAGCCCGATGCTGCGACGAGGCGACACCATCCGCTGGGCCGCGAGCCGCCCAGCCGCCGT
ACCATGGCCCCGTGACTTCG

Downstream 100 bases:

>100_bases
TGAGCCACGAACACGACGCCGGGGATCTCGACGCCCTCCGCGCTGAGATCGAGGCGGCAGAACGCCGCGTTGCGCGCGAA
ATCGAGCCAGGCGCGAGGGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRL
YGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSL
PSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR
GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRA
AIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFTFQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLE
ANRALVGQAVEVLVATGEGRKDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG
DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR

Sequences:

>Translated_512_residues
MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRL
YGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSL
PSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR
GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRA
AIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFTFQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLE
ANRALVGQAVEVLVATGEGRKDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG
DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR
>Mature_512_residues
MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRL
YGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSL
PSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR
GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRA
AIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFTFQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLE
ANRALVGQAVEVLVATGEGRKDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG
DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=36.1344537815126, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=36.1344537815126, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI93277076, Length=396, Percent_Identity=27.2727272727273, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=40.2684563758389, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=33.0337078651685, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17553146, Length=482, Percent_Identity=31.9502074688797, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=222, Percent_Identity=35.1351351351351, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21356207, Length=423, Percent_Identity=36.1702127659575, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI19922432, Length=400, Percent_Identity=30.25, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_MYCBO (P67086)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856398.1
- ProteinModelPortal:   P67086
- SMR:   P67086
- EnsemblBacteria:   EBMYCT00000015068
- GeneID:   1091778
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2752c
- GeneTree:   EBGT00050000015784
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14326
- BioCyc:   MBOV233413:MB2752C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 55086; Mature: 55086

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGS
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCC
EADVVVFNTCAVRENADNRLYGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWV
CCCEEEEEEEEECCCCCCCEECCHHHHCCHHCCCCCEEEEECCHHHCCHHHHHHHCCCCE
DVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSLPSSRESAYAAWVSISVGCNN
EEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCC
SCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILR
CHHHHHHHHHCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHH
AMRRSYRAERYLGIIERVRAAIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHEEE
FQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLEANRALVGQAVEVLVATGEGR
EEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
KDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG
CCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEHHHCCCCHHHHCCCHHHCCCCCCC
DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGS
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCC
EADVVVFNTCAVRENADNRLYGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWV
CCCEEEEEEEEECCCCCCCEECCHHHHCCHHCCCCCEEEEECCHHHCCHHHHHHHCCCCE
DVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSLPSSRESAYAAWVSISVGCNN
EEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEECCCC
SCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILR
CHHHHHHHHHCCCCCHHHEEECCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHH
AMRRSYRAERYLGIIERVRAAIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHEEE
FQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLEANRALVGQAVEVLVATGEGR
EEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
KDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG
CCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEEHHHCCCCHHHHCCCHHHCCCCCCC
DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972