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The map label for this gene is sthA

Identifier: 148823902

GI number: 148823902

Start: 3037396

End: 3038802

Strand: Direct

Name: sthA

Synonym: TBFG_12727

Alternate gene names: 148823902

Gene position: 3037396-3038802 (Clockwise)

Preceding gene: 148823900

Following gene: 148823903

Centisome position: 68.65

GC content: 63.54

Gene sequence:

>1407_bases
ATGCGTGAATACGACATCGTGGTGATCGGGTCAGGCCCGGGCGGACAGAAAGCCGCCATCGCCTCGGCGAAGCTGGGCAA
GTCCGTGGCCATCGTCGAACGCGGCCGAATGCTCGGCGGCGTCTGCGTCAACACAGGCACGATCCCATCCAAAACGTTGC
GTGAGGCTGTGCTCTACCTCACCGGCATGAACCAACGCGAGCTGTACGGCGCAAGCTACCGCGTGAAGGACCGGATCACC
CCGGCCGACCTGTTGGCGCGGACCCAGCACGTGATCGGCAAGGAAGTCGACGTGGTGCGCAACCAGCTGATGCGTAACCG
CGTCGATCTGATCGTGGGCCATGGCCGGTTCATCGACCCGCACACCATCCTCGTGGAGGACCAGGCCCGCAGGGAAAAGA
CCACCGTCACCGGCGACTACATCATCATCGCCACTGGCACCAGGCCGGCACGGCCATCCGGAGTCGAATTTGACGAAGAA
CGGGTGCTCGACTCCGACGGGATCCTCGATCTCAAATCGCTGCCATCCTCGATGGTCGTGGTCGGTGCCGGCGTGATCGG
CATCGAATACGCCTCCATGTTCGCTGCGTTGGGCACCAAAGTCACCGTCGTGGAGAAGCGGGACAACATGCTGGACTTCT
GCGACCCCGAGGTCGTCGAGGCGCTGAAATTCCACCTGCGCGACCTGGCGGTGACATTCCGGTTCGGCGAGGAAGTGACC
GCGGTCGATGTCGGCTCTGCGGGCACCGTGACCACCCTGGCCAGCGGCAAACAGATTCCAGCCGAGACCGTAATGTACTC
GGCGGGACGTCAGGGACAAACCGACCACCTCGACCTGCACAACGCCGGACTCGAGGTGCAGGGCCGCGGGCGGATCTTCG
TAGACGACCGTTTCCAGACCAAGGTAGACCACATCTACGCCGTCGGCGACGTCATTGGCTTCCCCGCCTTGGCCGCGACG
TCGATGGAGCAGGGGCGGCTGGCCGCCTACCACGCCTTCGGCGAACCAACCGACGGAATCACCGAACTTCAGCCGATCGG
TATTTATTCGATTCCCGAGGTGTCCTACGTCGGCGCCACCGAGGTGGAACTGACCAAGAGCTCCATCCCATACGAGGTGG
GAGTGGCCCGCTACCGGGAGCTGGCCCGCGGCCAAATCGCCGGCGACTCCTACGGCATGCTCAAGCTGCTGGTTTCCACC
GAGGATCTCAAGCTGCTCGGCGTGCATATCTTCGGCACCAGCGCCACCGAGATGGTGCACATCGGGCAGGCCGTGATGGG
ATGCGGGGGCAGCGTCGAGTACCTGGTCGACGCGGTGTTCAACTACCCGACCTTCTCGGAGGCCTACAAGAACGCCGCAC
TGGACGTGATGAACAAGATGCGCGCACTCAACCAGTTCCGCCGCTGA

Upstream 100 bases:

>100_bases
GACTACCGGCACCGTCAGCCGGTGCCCACGTCACGCGATCGAGCTGCCTTCCTGTGGACGAAGGCGTAACTGTGCGTTCT
ACTGTCATTTCATGGGGTCG

Downstream 100 bases:

>100_bases
GGGTGCCGAGCGGATGTGAATCCGTCTCGGCGCCCAAGTAGGCTTGCCAGCAAATTCGCCGCCGCCCACGAACGGTCGGC
GTCGAACGTGGCCCCGCGCT

Product: soluble pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MREYDIVVIGSGPGGQKAAIASAKLGKSVAIVERGRMLGGVCVNTGTIPSKTLREAVLYLTGMNQRELYGASYRVKDRIT
PADLLARTQHVIGKEVDVVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEE
RVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEVVEALKFHLRDLAVTFRFGEEVT
AVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGDVIGFPALAAT
SMEQGRLAAYHAFGEPTDGITELQPIGIYSIPEVSYVGATEVELTKSSIPYEVGVARYRELARGQIAGDSYGMLKLLVST
EDLKLLGVHIFGTSATEMVHIGQAVMGCGGSVEYLVDAVFNYPTFSEAYKNAALDVMNKMRALNQFRR

Sequences:

>Translated_468_residues
MREYDIVVIGSGPGGQKAAIASAKLGKSVAIVERGRMLGGVCVNTGTIPSKTLREAVLYLTGMNQRELYGASYRVKDRIT
PADLLARTQHVIGKEVDVVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEE
RVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEVVEALKFHLRDLAVTFRFGEEVT
AVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGDVIGFPALAAT
SMEQGRLAAYHAFGEPTDGITELQPIGIYSIPEVSYVGATEVELTKSSIPYEVGVARYRELARGQIAGDSYGMLKLLVST
EDLKLLGVHIFGTSATEMVHIGQAVMGCGGSVEYLVDAVFNYPTFSEAYKNAALDVMNKMRALNQFRR
>Mature_468_residues
MREYDIVVIGSGPGGQKAAIASAKLGKSVAIVERGRMLGGVCVNTGTIPSKTLREAVLYLTGMNQRELYGASYRVKDRIT
PADLLARTQHVIGKEVDVVRNQLMRNRVDLIVGHGRFIDPHTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEE
RVLDSDGILDLKSLPSSMVVVGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEVVEALKFHLRDLAVTFRFGEEVT
AVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQTKVDHIYAVGDVIGFPALAAT
SMEQGRLAAYHAFGEPTDGITELQPIGIYSIPEVSYVGATEVELTKSSIPYEVGVARYRELARGQIAGDSYGMLKLLVST
EDLKLLGVHIFGTSATEMVHIGQAVMGCGGSVEYLVDAVFNYPTFSEAYKNAALDVMNKMRALNQFRR

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=31.4410480349345, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=26.6375545851528, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI291045266, Length=481, Percent_Identity=27.2349272349272, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI22035672, Length=472, Percent_Identity=30.0847457627119, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI291045268, Length=467, Percent_Identity=26.1241970021413, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI33519430, Length=428, Percent_Identity=26.8691588785047, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI33519428, Length=428, Percent_Identity=26.8691588785047, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI33519426, Length=428, Percent_Identity=26.8691588785047, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI148277065, Length=428, Percent_Identity=26.8691588785047, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI148277071, Length=428, Percent_Identity=26.8691588785047, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=42.8571428571429, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786307, Length=457, Percent_Identity=32.3851203501094, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081717, Length=463, Percent_Identity=30.2375809935205, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789915, Length=448, Percent_Identity=28.125, Blast_Score=149, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=32.2510822510823, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=25.0517598343685, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=456, Percent_Identity=28.0701754385965, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=456, Percent_Identity=28.0701754385965, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=457, Percent_Identity=26.4770240700219, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=30.9322033898305, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=30.5907172995781, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=25.4310344827586, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=31.4775160599572, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=28.6012526096033, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=28.6610878661088, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=28.125, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): STHA_MYCBO (P66007)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856378.1
- ProteinModelPortal:   P66007
- SMR:   P66007
- EnsemblBacteria:   EBMYCT00000015518
- GeneID:   1091876
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2732
- GeneTree:   EBGT00050000014575
- HOGENOM:   HBG515043
- OMA:   EVGIARY
- ProtClustDB:   PRK05249
- BioCyc:   MBOV233413:MB2732-MONOMER
- BRENDA:   1.6.1.1
- GO:   GO:0005737
- HAMAP:   MF_00247
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962
- Gene3D:   G3DSA:3.30.390.30
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.6.1.1

Molecular weight: Translated: 50755; Mature: 50755

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYDIVVIGSGPGGQKAAIASAKLGKSVAIVERGRMLGGVCVNTGTIPSKTLREAVLYL
CCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCEECCEEEECCCCCHHHHHHHHHHH
TGMNQRELYGASYRVKDRITPADLLARTQHVIGKEVDVVRNQLMRNRVDLIVGHGRFIDP
CCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCEECC
HTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVV
EEEEECCCHHCCCCEECCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCEEHHHCCCCEEE
VGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEVVEALKFHLRDLAVTFRFGEEVT
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCEEE
AVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQT
EEECCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCEEEEECCCEEEECCCEEEECCCHHH
KVDHIYAVGDVIGFPALAATSMEQGRLAAYHAFGEPTDGITELQPIGIYSIPEVSYVGAT
HHHHEEEHHHHHCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEECCCC
EVELTKSSIPYEVGVARYRELARGQIAGDSYGMLKLLVSTEDLKLLGVHIFGTSATEMVH
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCHHHHHH
IGQAVMGCGGSVEYLVDAVFNYPTFSEAYKNAALDVMNKMRALNQFRR
HHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREYDIVVIGSGPGGQKAAIASAKLGKSVAIVERGRMLGGVCVNTGTIPSKTLREAVLYL
CCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCEECCEEEECCCCCHHHHHHHHHHH
TGMNQRELYGASYRVKDRITPADLLARTQHVIGKEVDVVRNQLMRNRVDLIVGHGRFIDP
CCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEECCCEECC
HTILVEDQARREKTTVTGDYIIIATGTRPARPSGVEFDEERVLDSDGILDLKSLPSSMVV
EEEEECCCHHCCCCEECCCEEEEEECCCCCCCCCCCCCHHHHCCCCCCEEHHHCCCCEEE
VGAGVIGIEYASMFAALGTKVTVVEKRDNMLDFCDPEVVEALKFHLRDLAVTFRFGEEVT
EECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCEEE
AVDVGSAGTVTTLASGKQIPAETVMYSAGRQGQTDHLDLHNAGLEVQGRGRIFVDDRFQT
EEECCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCEEEEECCCEEEECCCEEEECCCHHH
KVDHIYAVGDVIGFPALAATSMEQGRLAAYHAFGEPTDGITELQPIGIYSIPEVSYVGAT
HHHHEEEHHHHHCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEECCCC
EVELTKSSIPYEVGVARYRELARGQIAGDSYGMLKLLVSTEDLKLLGVHIFGTSATEMVH
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCHHHHHH
IGQAVMGCGGSVEYLVDAVFNYPTFSEAYKNAALDVMNKMRALNQFRR
HHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972