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The map label for this gene is ybjF [C]

Identifier: 148823879

GI number: 148823879

Start: 3017800

End: 3019017

Strand: Reverse

Name: ybjF [C]

Synonym: TBFG_12704

Alternate gene names: 148823879

Gene position: 3019017-3017800 (Counterclockwise)

Preceding gene: 148823880

Following gene: 148823878

Centisome position: 68.24

GC content: 67.9

Gene sequence:

>1218_bases
GTGACCCGCGCCGGCGACGATGCAGTGAATCTGACGTTGGTCACCGGAGCGCCCGCGAACGGCGGTAGCTGCGTGGCGCA
CCACGAGGGCCGGGTGGTTTTCGTCCGCTATGCGTTGCCCGGTGAACGAGTGCGGGCGCGGGTGACCGCGCAACGTGGCT
CTTACTGGCACGCAGAGGCTTTCGAGGTGATCGACCCGTCGCCCGACCGGATCGGGTCGCTGTGTTCGATCGCCGGGGCC
GACGGCGCCGGGTGTTGCGATCTGGCGTTTGCTGCTCCGGAGGCGGCCCGCACACTTAAGGCGCAAGTTGTGGCTAACCA
GCTGGAACGGCTCGGGCGGCACAGCTGGCAGGGCGAGGCGCAACCGCTGTCGGATGCCGGCCCCACGGGTTGGCGTATTC
GAGTCCGGCTCGACGTGGGCGCAGACCGTCGTCCAGGTTTTCACCGCTACCACAGTGGCGAGCTGGTAACCGACCTGGAC
TGCGGGCAGTTGCCTGTCGGCATGCTAGACGGCCTGGTAGCGGCTGACTGGCCGCCGGAAGCCCAGCTTTACGTGGCTCT
GGACGACGACGGCGAGCGCCATGTGGTCTGTTCCGTGCGGCAGGGCCCTCGGAATAGGACCCGAACCGTGACCAACGTGG
TGGAGGGCGCCTACCACGCCCACCAGCGTGTGCACCGGCGCAGCTGGCGGGTGCCGGTAACGGCCTTTTGGCAGGCGCAT
CGCGACGCGGCGGCGGTCTACAGCGATCTGATCGCTGACTGGGCTCAGCCCGCCCCCGGCATGACCGCCTGGGATCTCTA
CGGTGGTGCGGGGGTTTTCGCTGCGGTGCTGGGTGAGGCGGTCGGGGAGTCCGGACGGGTGTTGACCGTCGACACTTCGC
GCCTGGCATCCGGGGCCGCACGTGCCGCGCTGGTCGATTTGCCCCAGGTGGAGGTCGTCACCGGTTCGGTGCGGCGGGTG
TTGGCAGTGCAACCCGCCGGCGCGGATCTGGCGGTGCTGGATCCACCGCGATCAGGCGCTGGGCGCGAGGTTGTCGACTT
GCTGGCCGGTGCGGGGGTGCCACGGTTGATACATATCGGTTGCGAAGCGGCGTCTTTCGCGCGCGATATCGGCCTCTACC
GTGGTCACGGTTACGCCGTGGAGAAGATCAAGGTGTTCGACGCGTTCCCGCTGACTCACTACGTCGAGTGCGTTGCGCTG
TTGACCCGAAAGGTGTAG

Upstream 100 bases:

>100_bases
CGCGCCGGCGACGATGCAGAGCAAAGCGATGGGGAGGAGCGGCGCCAGTGACCCGCGCCGGCGACGATGCAGAGCAAAGC
GATGGGGAGGAGCGGCGCCA

Downstream 100 bases:

>100_bases
AGGTCATGCAGCTGCTCGACCAGCTCGCCCGTGGTCGTCGTGGGTTGCTCCGTATACAGCAGCGCAGCTAGTAGTCTTGG
GTTGGCGCTCCGGGAAGACT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEAFEVIDPSPDRIGSLCSIAGA
DGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEAQPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLD
CGQLPVGMLDGLVAADWPPEAQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAH
RDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAARAALVDLPQVEVVTGSVRRV
LAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIGCEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVAL
LTRKV

Sequences:

>Translated_405_residues
MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEAFEVIDPSPDRIGSLCSIAGA
DGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEAQPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLD
CGQLPVGMLDGLVAADWPPEAQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAH
RDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAARAALVDLPQVEVVTGSVRRV
LAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIGCEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVAL
LTRKV
>Mature_404_residues
TRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEAFEVIDPSPDRIGSLCSIAGAD
GAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEAQPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDC
GQLPVGMLDGLVAADWPPEAQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAHR
DAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAARAALVDLPQVEVVTGSVRRVL
AVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIGCEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALL
TRKV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Escherichia coli, GI1787083, Length=173, Percent_Identity=25.4335260115607, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 43242; Mature: 43111

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEA
CCCCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCHHEEEEEECCCCEECCCC
FEVIDPSPDRIGSLCSIAGADGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEA
CEECCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
QPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDCGQLPVGMLDGLVAADWPPE
CCCCCCCCCCEEEEEEEECCCCCCCCCCEECCCCEEEECCCCCCCHHHHCCCCCCCCCCC
AQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAH
CEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAA
HHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHH
RAALVDLPQVEVVTGSVRRVLAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIG
HHHEECCCCEEEEECCCEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEC
CEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALLTRKV
CCHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEA
CCCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCHHEEEEEECCCCEECCCC
FEVIDPSPDRIGSLCSIAGADGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEA
CEECCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
QPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDCGQLPVGMLDGLVAADWPPE
CCCCCCCCCCEEEEEEEECCCCCCCCCCEECCCCEEEECCCCCCCHHHHCCCCCCCCCCC
AQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAH
CEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAA
HHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHH
RAALVDLPQVEVVTGSVRRVLAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIG
HHHEECCCCEEEEECCCEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEC
CEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALLTRKV
CCHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]