Click here to switch to the map view.

The map label for this gene is intF [C]

Identifier: 148823850

GI number: 148823850

Start: 2991650

End: 2992777

Strand: Reverse

Name: intF [C]

Synonym: TBFG_12675

Alternate gene names: 148823850

Gene position: 2992777-2991650 (Counterclockwise)

Preceding gene: 148823851

Following gene: 148823849

Centisome position: 67.64

GC content: 64.89

Gene sequence:

>1128_bases
GTGACGCAAACCGGCAAGCGTCAGAGACGCAAATTCGGTCGCATCCGACAGTTCAACTCCGGCCGCTGGCAAGCCAGCTA
CACCGGCCCCGACGGCCGCGTGTACATCGCCCCCAAAACCTTCAACGCCAAGATCGACGCCGAAGCATGGCTCACCGACC
GCCGCCGCGAAATCGACCGACAACTATGGTCCCCGGCATCGGGTCAGGAAGACCGCCCCGGAGCCCCATTCGGTGAGTAC
GCCGAAGGATGGCTGAAGCAGCGTGGAATCAAGGACCGCACCCGCGCCCACTATCGCAAACTGCTGGACAACCACATCCT
GGCCACCTTCGCTGACACCGACCTACGCGACATCACCCCGGCCGCCGTGCGCCGCTGGTACGCCACCACCGCCGTGGGCA
CACCGACCATGCGGGCACACTCCTACAGCTTGCTGCGCGCAATCATGCAGACCGCCTTGGCCGACGACCTGATCGACTCC
AACCCCTGCCGCATCTCAGGCGCGTCCACCGCCCGCCGCGTCCACAAGATCAGGCCCGCCACCCTCGACGAGCTGGAAAC
CATCACCAAAGCCATGCCCGACCCCTACCAGGCGTTCGTGCTGATGGCGGCATGGCTGGCCATGCGCTACGGCGAGCTGA
CCGAATTACGCCGCAAAGACATCGACCTGCACGGCGAGGTTGCGCGGGTGCGGCGGGCTGTCGTTCGGGTGGGCGAAGGC
TTCAAGGTGACGACACCGAAAAGCGATGCGGGAGTGCGCGACATAAGTATCCCGCCACATCTGATACCCGCCATCGAAGA
CCACCTTCACAAACACGTCAACCCCGGCCGGGAGTCCCTGCTGTTCCCATCGGTCAACGACCCCAACCGTCACCTAGCAC
CCTCGGCGCTGTACCGCATGTTCTACAAGGCCCGAAAAGCCGCCGGCCGACCAGACTTACGGGTGCACGACCTTCGACAC
TCCGGCGCCGTGTTGGCTGCATCCACCGGCGCCACACTGGCCGAACTGATGCAGCGGCTAGGACACAGCACAGCCGGCGC
CGCACTCCGCTACCAGCACGCCGCCAAGGGCCGGGACCGCGAAATCGCCGCACTGTTAAGCAAACTGGCCGAGAACCAGG
AGATGTGA

Upstream 100 bases:

>100_bases
CAGGCGACATAGACAACAGTACCCCCGGAAGCGTGCGGAAGCAAGCGGCATCTGATGTAGTCTCTTATTGCGCGTTTATT
GCGCGGGACAGGGGACGACA

Downstream 100 bases:

>100_bases
TGATGGCCGATGCGGTTAAGTACGTAGTTATGTGCAACTGCGACGACGAACCGGGAGCGCTCATCATCGCCTGGATCGAC
GACGAACGACCCGCCGGCGG

Product: phiRv2 prophage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTQTGKRQRRKFGRIRQFNSGRWQASYTGPDGRVYIAPKTFNAKIDAEAWLTDRRREIDRQLWSPASGQEDRPGAPFGEY
AEGWLKQRGIKDRTRAHYRKLLDNHILATFADTDLRDITPAAVRRWYATTAVGTPTMRAHSYSLLRAIMQTALADDLIDS
NPCRISGASTARRVHKIRPATLDELETITKAMPDPYQAFVLMAAWLAMRYGELTELRRKDIDLHGEVARVRRAVVRVGEG
FKVTTPKSDAGVRDISIPPHLIPAIEDHLHKHVNPGRESLLFPSVNDPNRHLAPSALYRMFYKARKAAGRPDLRVHDLRH
SGAVLAASTGATLAELMQRLGHSTAGAALRYQHAAKGRDREIAALLSKLAENQEM

Sequences:

>Translated_375_residues
MTQTGKRQRRKFGRIRQFNSGRWQASYTGPDGRVYIAPKTFNAKIDAEAWLTDRRREIDRQLWSPASGQEDRPGAPFGEY
AEGWLKQRGIKDRTRAHYRKLLDNHILATFADTDLRDITPAAVRRWYATTAVGTPTMRAHSYSLLRAIMQTALADDLIDS
NPCRISGASTARRVHKIRPATLDELETITKAMPDPYQAFVLMAAWLAMRYGELTELRRKDIDLHGEVARVRRAVVRVGEG
FKVTTPKSDAGVRDISIPPHLIPAIEDHLHKHVNPGRESLLFPSVNDPNRHLAPSALYRMFYKARKAAGRPDLRVHDLRH
SGAVLAASTGATLAELMQRLGHSTAGAALRYQHAAKGRDREIAALLSKLAENQEM
>Mature_374_residues
TQTGKRQRRKFGRIRQFNSGRWQASYTGPDGRVYIAPKTFNAKIDAEAWLTDRRREIDRQLWSPASGQEDRPGAPFGEYA
EGWLKQRGIKDRTRAHYRKLLDNHILATFADTDLRDITPAAVRRWYATTAVGTPTMRAHSYSLLRAIMQTALADDLIDSN
PCRISGASTARRVHKIRPATLDELETITKAMPDPYQAFVLMAAWLAMRYGELTELRRKDIDLHGEVARVRRAVVRVGEGF
KVTTPKSDAGVRDISIPPHLIPAIEDHLHKHVNPGRESLLFPSVNDPNRHLAPSALYRMFYKARKAAGRPDLRVHDLRHS
GAVLAASTGATLAELMQRLGHSTAGAALRYQHAAKGRDREIAALLSKLAENQEM

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): INT2_MYCTU (P71956)

Other databases:

- EMBL:   BX842580
- EMBL:   AE000516
- PIR:   G70966
- RefSeq:   NP_217175.1
- RefSeq:   NP_337236.1
- ProteinModelPortal:   P71956
- EnsemblBacteria:   EBMYCT00000001500
- EnsemblBacteria:   EBMYCT00000069072
- GeneID:   885098
- GeneID:   925565
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2735
- KEGG:   mtu:Rv2659c
- TIGR:   MT2735
- TubercuList:   Rv2659c
- GeneTree:   EBGT00070000032160
- HOGENOM:   HBG564848
- OMA:   RGTYYFR
- ProtClustDB:   CLSK791984
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109
- Gene3D:   G3DSA:1.10.150.130
- Gene3D:   G3DSA:1.10.443.10

Pfam domain/function: PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz

EC number: NA

Molecular weight: Translated: 42177; Mature: 42045

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: NA

Important sites: ACT_SITE 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTGKRQRRKFGRIRQFNSGRWQASYTGPDGRVYIAPKTFNAKIDAEAWLTDRRREIDR
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
QLWSPASGQEDRPGAPFGEYAEGWLKQRGIKDRTRAHYRKLLDNHILATFADTDLRDITP
HHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHCCH
AAVRRWYATTAVGTPTMRAHSYSLLRAIMQTALADDLIDSNPCRISGASTARRVHKIRPA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCC
TLDELETITKAMPDPYQAFVLMAAWLAMRYGELTELRRKDIDLHGEVARVRRAVVRVGEG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
FKVTTPKSDAGVRDISIPPHLIPAIEDHLHKHVNPGRESLLFPSVNDPNRHLAPSALYRM
CEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHCEECCCCCCCCCCCCHHHHHHH
FYKARKAAGRPDLRVHDLRHSGAVLAASTGATLAELMQRLGHSTAGAALRYQHAAKGRDR
HHHHHHHCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
EIAALLSKLAENQEM
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQTGKRQRRKFGRIRQFNSGRWQASYTGPDGRVYIAPKTFNAKIDAEAWLTDRRREIDR
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHH
QLWSPASGQEDRPGAPFGEYAEGWLKQRGIKDRTRAHYRKLLDNHILATFADTDLRDITP
HHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHCCH
AAVRRWYATTAVGTPTMRAHSYSLLRAIMQTALADDLIDSNPCRISGASTARRVHKIRPA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCC
TLDELETITKAMPDPYQAFVLMAAWLAMRYGELTELRRKDIDLHGEVARVRRAVVRVGEG
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
FKVTTPKSDAGVRDISIPPHLIPAIEDHLHKHVNPGRESLLFPSVNDPNRHLAPSALYRM
CEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCHHCEECCCCCCCCCCCCHHHHHHH
FYKARKAAGRPDLRVHDLRHSGAVLAASTGATLAELMQRLGHSTAGAALRYQHAAKGRDR
HHHHHHHCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCH
EIAALLSKLAENQEM
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036