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The map label for this gene is pimA

Identifier: 148823804

GI number: 148823804

Start: 2951182

End: 2952318

Strand: Reverse

Name: pimA

Synonym: TBFG_12629

Alternate gene names: 148823804

Gene position: 2952318-2951182 (Counterclockwise)

Preceding gene: 148823805

Following gene: 148823803

Centisome position: 66.73

GC content: 66.23

Gene sequence:

>1137_bases
ATGCGGATCGGCATGATTTGTCCGTACTCGTTCGACGTCCCGGGCGGGGTGCAGTCGCATGTGCTACAGCTTGCCGAGGT
GATGCGCACCCGCGGCCACTTGGTCAGTGTGCTCGCGCCGGCCTCCCCGCATGCCGCACTGCCTGATTACTTTGTCTCCG
GTGGTAGGGCGGTTCCGATTCCCTACAACGGCTCGGTGGCCCGGCTGCGGTTCGGTCCGGCGACCCACCGCAAGGTCAAA
AAGTGGCTTGCGCATGGTGATTTCGACGTACTGCATCTACACGAGCCGAATGCGCCGAGCCTGTCGATGCTGGCCCTGAA
CATTGCCGAGGGCCCGATTGTGGCGACATTTCACACCTCGACCACCAAGTCGCTGACGCTGACGGTGTTCCAGGGCATTC
TGCGGCCCATGCACGAGAAGATCGTCGGCCGGATCGCGGTGTCCGACCTAGCCCGGCGCTGGCAGATGGAGGCGTTGGGA
TCCGATGCGGTGGAGATCCCCAATGGGGTGGACGTTGATTCCTTCGCCTCGGCAGCGCGGCTGGACGGGTACCCGCGCCA
GGGTAAAACGGTGTTGTTCCTGGGTCGCTACGACGAGCCCCGCAAGGGCATGGCCGTTTTGCTCGACGCGCTGCCGAAGG
TGGTGCAGCGGTTTCCGGATGTCCAGCTACTGATCGTCGGCCACGGCGACGCCGACCAGTTGCGCGGCCAGGCGGGCCGT
TTGGCGGCGCACCTGCGCTTTCTGGGTCAGGTGGACGACGCCGGAAAAGCTTCGGCGATGCGCAGCGCCGACGTCTACTG
TGCGCCCAACACCGGCGGTGAGAGTTTCGGCATTGTGCTGGTCGAAGCGATGGCCGCCGGCACTGCGGTGGTGGCCAGCG
ACCTCGACGCCTTCCGGCGTGTGCTGCGCGACGGTGAGGTCGGGCACCTGGTGCCGGTGGACCCGCCAGACTTGCAGGCC
GCCGCGTTGGCCGATGGACTGATTGCGGTGCTGGAGAACGATGTCCTGCGGGAGCGCTATGTGGCGGCCGGCAACGCGGC
CGTCCGCCGGTATGACTGGTCGGTGGTGGCCAGCCAGATCATGCGAGTGTACGAGACGGTCGCCGGGTCGGGCGCCAAGG
TTCAGGTGGCCAGCTGA

Upstream 100 bases:

>100_bases
GCCGCCCATCCCGCGGATTGGCACATGCTGCAGCCCCAGTGGCTGGCCGACTTATCCGAATCGCGGCGGGCGCAACTGAG
GTCCAGATGAGGAAAGCCTG

Downstream 100 bases:

>100_bases
TGACGTGGCTGGTCCTGGCCGGCGCAGTGCTGCTCGTTGTGCTGGTTGCGTTCGGCGCCTGGGGATATCAGACGGCCAAC
CGGTTGAACCGGTTGAACGT

Product: alpha-mannosyltransferase pimA

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MRIGMICPYSFDVPGGVQSHVLQLAEVMRTRGHLVSVLAPASPHAALPDYFVSGGRAVPIPYNGSVARLRFGPATHRKVK
KWLAHGDFDVLHLHEPNAPSLSMLALNIAEGPIVATFHTSTTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALG
SDAVEIPNGVDVDSFASAARLDGYPRQGKTVLFLGRYDEPRKGMAVLLDALPKVVQRFPDVQLLIVGHGDADQLRGQAGR
LAAHLRFLGQVDDAGKASAMRSADVYCAPNTGGESFGIVLVEAMAAGTAVVASDLDAFRRVLRDGEVGHLVPVDPPDLQA
AALADGLIAVLENDVLRERYVAAGNAAVRRYDWSVVASQIMRVYETVAGSGAKVQVAS

Sequences:

>Translated_378_residues
MRIGMICPYSFDVPGGVQSHVLQLAEVMRTRGHLVSVLAPASPHAALPDYFVSGGRAVPIPYNGSVARLRFGPATHRKVK
KWLAHGDFDVLHLHEPNAPSLSMLALNIAEGPIVATFHTSTTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALG
SDAVEIPNGVDVDSFASAARLDGYPRQGKTVLFLGRYDEPRKGMAVLLDALPKVVQRFPDVQLLIVGHGDADQLRGQAGR
LAAHLRFLGQVDDAGKASAMRSADVYCAPNTGGESFGIVLVEAMAAGTAVVASDLDAFRRVLRDGEVGHLVPVDPPDLQA
AALADGLIAVLENDVLRERYVAAGNAAVRRYDWSVVASQIMRVYETVAGSGAKVQVAS
>Mature_378_residues
MRIGMICPYSFDVPGGVQSHVLQLAEVMRTRGHLVSVLAPASPHAALPDYFVSGGRAVPIPYNGSVARLRFGPATHRKVK
KWLAHGDFDVLHLHEPNAPSLSMLALNIAEGPIVATFHTSTTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALG
SDAVEIPNGVDVDSFASAARLDGYPRQGKTVLFLGRYDEPRKGMAVLLDALPKVVQRFPDVQLLIVGHGDADQLRGQAGR
LAAHLRFLGQVDDAGKASAMRSADVYCAPNTGGESFGIVLVEAMAAGTAVVASDLDAFRRVLRDGEVGHLVPVDPPDLQA
AALADGLIAVLENDVLRERYVAAGNAAVRRYDWSVVASQIMRVYETVAGSGAKVQVAS

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1)

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMA_MYCBO (Q7TY88)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856288.1
- ProteinModelPortal:   Q7TY88
- SMR:   Q7TY88
- EnsemblBacteria:   EBMYCT00000014803
- GeneID:   1091982
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2642c
- GeneTree:   EBGT00050000015521
- HOGENOM:   HBG726846
- OMA:   SEEARRW
- ProtClustDB:   CLSK872083
- BioCyc:   MBOV233413:MB2642C-MONOMER
- BioCyc:   MetaCyc:MONOMER-7717
- BRENDA:   2.4.1.57
- InterPro:   IPR001296

Pfam domain/function: PF00534 Glycos_transf_1

EC number: =2.4.1.57

Molecular weight: Translated: 40446; Mature: 40446

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: BINDING 9-9 BINDING 16-16 BINDING 196-196 BINDING 256-256 BINDING 282-282

Signals:

None

Transmembrane regions:

HASH(0x1b7b307c)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGMICPYSFDVPGGVQSHVLQLAEVMRTRGHLVSVLAPASPHAALPDYFVSGGRAVPI
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHCCCEEEEC
PYNGSVARLRFGPATHRKVKKWLAHGDFDVLHLHEPNAPSLSMLALNIAEGPIVATFHTS
CCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEECC
TTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALGSDAVEIPNGVDVDSFASAAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHH
LDGYPRQGKTVLFLGRYDEPRKGMAVLLDALPKVVQRFPDVQLLIVGHGDADQLRGQAGR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCHHHH
LAAHLRFLGQVDDAGKASAMRSADVYCAPNTGGESFGIVLVEAMAAGTAVVASDLDAFRR
HHHHHHHHHCCCCCCHHHHHHCCCEEECCCCCCCCHHHHEEEHHHCCHHHHHHHHHHHHH
VLRDGEVGHLVPVDPPDLQAAALADGLIAVLENDVLRERYVAAGNAAVRRYDWSVVASQI
HHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHH
MRVYETVAGSGAKVQVAS
HHHHHHHCCCCCEEEECH
>Mature Secondary Structure
MRIGMICPYSFDVPGGVQSHVLQLAEVMRTRGHLVSVLAPASPHAALPDYFVSGGRAVPI
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHCCCEEEEC
PYNGSVARLRFGPATHRKVKKWLAHGDFDVLHLHEPNAPSLSMLALNIAEGPIVATFHTS
CCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEECC
TTKSLTLTVFQGILRPMHEKIVGRIAVSDLARRWQMEALGSDAVEIPNGVDVDSFASAAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHH
LDGYPRQGKTVLFLGRYDEPRKGMAVLLDALPKVVQRFPDVQLLIVGHGDADQLRGQAGR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHCCHHHH
LAAHLRFLGQVDDAGKASAMRSADVYCAPNTGGESFGIVLVEAMAAGTAVVASDLDAFRR
HHHHHHHHHCCCCCCHHHHHHCCCEEECCCCCCCCHHHHEEEHHHCCHHHHHHHHHHHHH
VLRDGEVGHLVPVDPPDLQAAALADGLIAVLENDVLRERYVAAGNAAVRRYDWSVVASQI
HHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHH
MRVYETVAGSGAKVQVAS
HHHHHHHCCCCCEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972