Click here to switch to the map view.

The map label for this gene is pdxT

Identifier: 148823798

GI number: 148823798

Start: 2945010

End: 2945606

Strand: Reverse

Name: pdxT

Synonym: TBFG_12623

Alternate gene names: 148823798

Gene position: 2945606-2945010 (Counterclockwise)

Preceding gene: 148823799

Following gene: 148823797

Centisome position: 66.58

GC content: 68.01

Gene sequence:

>597_bases
GTGAGCGTTCCACGGGTCGGGGTGCTGGCGCTGCAGGGCGACACCCGGGAGCACCTGGCTGCGCTGCGCGAATGCGGGGC
CGAGCCGATGACGGTGCGGCGCCGCGACGAACTTGACGCGGTGGACGCGCTGGTCATCCCGGGCGGGGAATCCACCACGA
TGAGCCACCTGCTGCTCGACCTCGACCTGCTGGGACCGCTGCGGGCCCGGCTCGCCGATGGGCTTCCGGCCTATGGTTCG
TGCGCGGGCATGATTCTGTTGGCCAGCGAGATCCTGGACGCCGGTGCGGCAGGCCGCCAGGCGCTGCCCCTGCGTGCGAT
GAATATGACGGTGCGGCGCAATGCTTTTGGAAGTCAGGTTGACTCGTTTGAAGGCGATATCGAGTTCGCTGGTCTAGACG
ATCCGGTGCGCGCGGTGTTCATCCGGGCGCCATGGGTTGAGCGAGTCGGTGACGGTGTGCAGGTGCTGGCCCGCGCGGCG
GGGCACATCGTCGCGGTGCGCCAGGGTGCGGTGCTTGCCACCGCGTTTCATCCGGAGATGACCGGCGATCGCCGCATTCA
TCAGTTGTTCGTCGACATCGTCACCTCCGCGGCGTGA

Upstream 100 bases:

>100_bases
GAGATTTTCACACGGTCCGGGGAGATGGTCGCGGCGGTCATGCAGGAGGGGCTGACCCGCCACCGGCGCGGGCACCGGTC
GGTGGGGCAGTGAGCGGGCT

Downstream 100 bases:

>100_bases
GCAGTCGCAAAAGCACCCGTTTCAGGCCGAAATTTGGAGCTTTTGTGTCTGCTCGTGCCGGTTGGCTCGCCGCCCACAAC
CCCCACGTAGACTCGTCTGG

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSVPRVGVLALQGDTREHLAALRECGAEPMTVRRRDELDAVDALVIPGGESTTMSHLLLDLDLLGPLRARLADGLPAYGS
CAGMILLASEILDAGAAGRQALPLRAMNMTVRRNAFGSQVDSFEGDIEFAGLDDPVRAVFIRAPWVERVGDGVQVLARAA
GHIVAVRQGAVLATAFHPEMTGDRRIHQLFVDIVTSAA

Sequences:

>Translated_198_residues
MSVPRVGVLALQGDTREHLAALRECGAEPMTVRRRDELDAVDALVIPGGESTTMSHLLLDLDLLGPLRARLADGLPAYGS
CAGMILLASEILDAGAAGRQALPLRAMNMTVRRNAFGSQVDSFEGDIEFAGLDDPVRAVFIRAPWVERVGDGVQVLARAA
GHIVAVRQGAVLATAFHPEMTGDRRIHQLFVDIVTSAA
>Mature_197_residues
SVPRVGVLALQGDTREHLAALRECGAEPMTVRRRDELDAVDALVIPGGESTTMSHLLLDLDLLGPLRARLADGLPAYGSC
AGMILLASEILDAGAAGRQALPLRAMNMTVRRNAFGSQVDSFEGDIEFAGLDDPVRAVFIRAPWVERVGDGVQVLARAAG
HIVAVRQGAVLATAFHPEMTGDRRIHQLFVDIVTSAA

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=211, Percent_Identity=35.5450236966825, Blast_Score=105, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323995, Length=213, Percent_Identity=35.6807511737089, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=213, Percent_Identity=35.2112676056338, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_MYCBO (Q7TY92)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856282.1
- ProteinModelPortal:   Q7TY92
- SMR:   Q7TY92
- EnsemblBacteria:   EBMYCT00000015404
- GeneID:   1091989
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2636c
- GeneTree:   EBGT00050000016176
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   MBOV233413:MB2636C-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21073; Mature: 20941

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 81-81 ACT_SITE 177-177 ACT_SITE 179-179 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPRVGVLALQGDTREHLAALRECGAEPMTVRRRDELDAVDALVIPGGESTTMSHLLLD
CCCCCEEEEEECCCCHHHHHHHHHCCCCCCEECCCCCCCHHCEEEECCCCCHHHHHHHHH
LDLLGPLRARLADGLPAYGSCAGMILLASEILDAGAAGRQALPLRAMNMTVRRNAFGSQV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHH
DSFEGDIEFAGLDDPVRAVFIRAPWVERVGDGVQVLARAAGHIVAVRQGAVLATAFHPEM
HCCCCCEEEECCCCCHHEEEEECCHHHHHCCHHHHHHHHHCCEEEEECCEEEEEEECCCC
TGDRRIHQLFVDIVTSAA
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVPRVGVLALQGDTREHLAALRECGAEPMTVRRRDELDAVDALVIPGGESTTMSHLLLD
CCCCEEEEEECCCCHHHHHHHHHCCCCCCEECCCCCCCHHCEEEECCCCCHHHHHHHHH
LDLLGPLRARLADGLPAYGSCAGMILLASEILDAGAAGRQALPLRAMNMTVRRNAFGSQV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHH
DSFEGDIEFAGLDDPVRAVFIRAPWVERVGDGVQVLARAAGHIVAVRQGAVLATAFHPEM
HCCCCCEEEECCCCCHHEEEEECCHHHHHCCHHHHHHHHHCCEEEEECCEEEEEEECCCC
TGDRRIHQLFVDIVTSAA
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972