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The map label for this gene is echA14

Identifier: 148823682

GI number: 148823682

Start: 2807666

End: 2808436

Strand: Direct

Name: echA14

Synonym: TBFG_12507

Alternate gene names: 148823682

Gene position: 2807666-2808436 (Clockwise)

Preceding gene: 148823672

Following gene: 148823689

Centisome position: 63.46

GC content: 67.7

Gene sequence:

>771_bases
ATGGCCCAATACGACCCGGTCTTGCTCAGCGTCGACAAGCACGTTGCGCTCATCACGGTCAACGACCCGGACCGACGGAA
CGCCGTCACCGACGAGATGTCGGCGCAGTTGCGTGCGGCGATCCAACGCGCCGAAGGCGACCCCGACGTACACGCCGTAG
TCGTGACCGGGGCGGGCAAGGCCTTCTGCGCCGGGGCCGACCTGAGTGCGCTGGGCGCCGGGGTCGGCGATCCAGCCGAG
CCGAGATTGTTACGGCTCTACGACGGTTTCATGGCCGTCAGTAGTTGTAATCTGCCCACCATCGCCGCGGTCAACGGCGC
GGCTGTGGGCGCCGGACTCAATCTGGCGTTGGCCGCCGATGTGCGCATCGCCGGACCGGCCGCATTGTTCGACGCCCGCT
TCCAAAAGCTGGGACTGCATCCAGGTGGCGGCGCAACCTGGATGCTGCAGCGAGCGGTGGGTCCGCAGGTCGCCCGTGCG
GCCTTATTGTTCGGCATGTGCTTCGACGCCGAATCCGCTGTGCGGCACGGCTTGGCGCTAATGGTTGCCGACGATCCCGT
CACCGCGGCGCTGGAGCTGGCCGCCGGGCCCGCAGCCGCCCCGCGCGAGGTCGTGCTGGCGAGCAAAGCCACCATGCGCG
CCACAGCCAGCCCCGGATCGCTGGACCTTGAGCAACACGAACTCGCCAAACGCTTAGAACTTGGGCCGCAGGCGAAATCG
GTCCAGTCGCCCGAGTTCGCCGCTCGCTTGGCTGCCGCTCAACACAGGTAG

Upstream 100 bases:

>100_bases
CTGTGGAAATAGCAGGTCAGCATGGTGGCGTGGCTTGACACCGCCTCGTTATGGGTCGACGCCCAGAGTCGCCTTCAAAC
TCAAACCACGGAGGTGCCCG

Downstream 100 bases:

>100_bases
CGCCTACCAGCCTCGAGGGTTTCCATGGCGTGCCCCAGTCCGAAGCTGCTGCTGCTTGACTCCGCGCGCTGGGCCCGAGC
GCGCGCTGTTGTACGGCCCA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAQYDPVLLSVDKHVALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVVTGAGKAFCAGADLSALGAGVGDPAE
PRLLRLYDGFMAVSSCNLPTIAAVNGAAVGAGLNLALAADVRIAGPAALFDARFQKLGLHPGGGATWMLQRAVGPQVARA
ALLFGMCFDAESAVRHGLALMVADDPVTAALELAAGPAAAPREVVLASKATMRATASPGSLDLEQHELAKRLELGPQAKS
VQSPEFAARLAAAQHR

Sequences:

>Translated_256_residues
MAQYDPVLLSVDKHVALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVVTGAGKAFCAGADLSALGAGVGDPAE
PRLLRLYDGFMAVSSCNLPTIAAVNGAAVGAGLNLALAADVRIAGPAALFDARFQKLGLHPGGGATWMLQRAVGPQVARA
ALLFGMCFDAESAVRHGLALMVADDPVTAALELAAGPAAAPREVVLASKATMRATASPGSLDLEQHELAKRLELGPQAKS
VQSPEFAARLAAAQHR
>Mature_255_residues
AQYDPVLLSVDKHVALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVVTGAGKAFCAGADLSALGAGVGDPAEP
RLLRLYDGFMAVSSCNLPTIAAVNGAAVGAGLNLALAADVRIAGPAALFDARFQKLGLHPGGGATWMLQRAVGPQVARAA
LLFGMCFDAESAVRHGLALMVADDPVTAALELAAGPAAAPREVVLASKATMRATASPGSLDLEQHELAKRLELGPQAKSV
QSPEFAARLAAAQHR

Specific function: Could possibly oxidize fatty acids using specific components

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=206, Percent_Identity=34.4660194174757, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI37594469, Length=232, Percent_Identity=28.8793103448276, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI37594471, Length=232, Percent_Identity=28.8793103448276, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI70995211, Length=166, Percent_Identity=29.5180722891566, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI45643119, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI260275230, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI68989263, Length=206, Percent_Identity=31.0679611650485, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI213417737, Length=221, Percent_Identity=25.7918552036199, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI157694516, Length=221, Percent_Identity=25.7918552036199, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=34.1176470588235, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787660, Length=204, Percent_Identity=38.2352941176471, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788597, Length=171, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788682, Length=206, Percent_Identity=32.0388349514563, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI221142681, Length=173, Percent_Identity=31.7919075144509, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=217, Percent_Identity=32.7188940092166, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=167, Percent_Identity=36.5269461077844, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=217, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=185, Percent_Identity=29.1891891891892, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=201, Percent_Identity=33.8308457711443, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=186, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=178, Percent_Identity=32.5842696629214, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20129971, Length=184, Percent_Identity=35.8695652173913, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653477, Length=184, Percent_Identity=35.8695652173913, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920382, Length=175, Percent_Identity=29.1428571428571, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650670, Length=174, Percent_Identity=32.183908045977, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653139, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571730, Length=150, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28571729, Length=150, Percent_Identity=29.3333333333333, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357171, Length=133, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ECH14_MYCBO (P64019)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856158.1
- ProteinModelPortal:   P64019
- SMR:   P64019
- EnsemblBacteria:   EBMYCT00000015504
- GeneID:   1094201
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2511
- GeneTree:   EBGT00050000015506
- HOGENOM:   HBG748731
- OMA:   ARSIESP
- ProtClustDB:   PRK05870
- BioCyc:   MBOV233413:MB2511-MONOMER
- BRENDA:   4.2.1.17
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 26280; Mature: 26149

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYDPVLLSVDKHVALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVVTGAGK
CCCCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFCAGADLSALGAGVGDPAEPRLLRLYDGFMAVSSCNLPTIAAVNGAAVGAGLNLALAAD
EEECCCCHHHHCCCCCCCCCCCEEEEHHHHHHEECCCCCEEEEECCCEECCCCEEEEEEC
VRIAGPAALFDARFQKLGLHPGGGATWMLQRAVGPQVARAALLFGMCFDAESAVRHGLAL
EEECCHHHHHHHHHHHHCCCCCCCHHEEEEHHCCHHHHHHHHHHHHHCCHHHHHHCCEEE
MVADDPVTAALELAAGPAAAPREVVLASKATMRATASPGSLDLEQHELAKRLELGPQAKS
EEECCCHHHHHEECCCCCCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
VQSPEFAARLAAAQHR
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AQYDPVLLSVDKHVALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVVTGAGK
CCCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
AFCAGADLSALGAGVGDPAEPRLLRLYDGFMAVSSCNLPTIAAVNGAAVGAGLNLALAAD
EEECCCCHHHHCCCCCCCCCCCEEEEHHHHHHEECCCCCEEEEECCCEECCCCEEEEEEC
VRIAGPAALFDARFQKLGLHPGGGATWMLQRAVGPQVARAALLFGMCFDAESAVRHGLAL
EEECCHHHHHHHHHHHHCCCCCCCHHEEEEHHCCHHHHHHHHHHHHHCCHHHHHHCCEEE
MVADDPVTAALELAAGPAAAPREVVLASKATMRATASPGSLDLEQHELAKRLELGPQAKS
EEECCCHHHHHEECCCCCCCCCCEEEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCC
VQSPEFAARLAAAQHR
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972