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The map label for this gene is tig [H]

Identifier: 148823662

GI number: 148823662

Start: 2778565

End: 2779965

Strand: Reverse

Name: tig [H]

Synonym: TBFG_12487

Alternate gene names: 148823662

Gene position: 2779965-2778565 (Counterclockwise)

Preceding gene: 148823664

Following gene: 148823661

Centisome position: 62.83

GC content: 65.1

Gene sequence:

>1401_bases
GTGAAGAGCACCGTCGAGCAGTTGAGCCCCACCCGGGTTCGTATCAACGTGGAGGTGCCATTCGCCGAGCTTGAGCCGGA
TTTCCAGCGGGCCTACAAAGAGCTGGCCAAACAGGTGCGGCTGCCCGGCTTCCGGCCCGGGAAGGCGCCGGCCAAACTAC
TCGAAGCCCGCATCGGCCGGGAGGCCATGCTGGATCAAATCGTCAACGATGCGCTGCCCAGCCGGTACGGACAGGCGGTG
GCCGAGTCGGATGTCCAACCGCTCGGCCGGCCCAACATCGAGGTGACCAAGAAGGAGTACGGCCAGGACCTGCAATTCAC
CGCCGAGGTCGACATCCGCCCGAAGATCAGTCCCCCGGACCTGAGCGCGCTGACGGTCTCGGTGGATCCGATCGAAATCG
GTGAGGACGACGTCGACGCCGAACTGCAGTCGTTACGTACCCGGTTCGGCACCCTGACCGCGGTGGACCGGCCGGTGGCC
GTCGGCGACGTCGTCTCGATCGACTTGTCTGCCACGGTCGACGGAGAGGACATACCGAACGCAGCCGCTGAGGGACTCTC
CCACGAGGTCGGCTCCGGCCGGCTCATCGCAGGTCTCGACGACGCGGTTGTTGGTCTGTCCGCCGACGAGTCCCGGGTCT
TCACCGCCAAGCTGGCAGCCGGCGAGCACGCCGGGCAGGAAGCTCAGGTTACCGTCACGGTCAGGTCGGTTAAGGAGCGC
GAACTACCAGAGCCCGACGACGAATTCGCGCAGTTAGCCAGCGAGTTCGACAGCATCGACGAATTGCGGGCCAGCCTCAG
CGACCAGGTGCGCCAGGCCAAGCGCGCCCAGCAGGCCGAGCAGATTCGAAACGCCACCATCGATGCGCTACTCGAACAGG
TCGACGTGCCGTTGCCGGAGTCGTATGTGCAGGCCCAATTCGACAGCGTGCTGCACAGCGCGCTCAGCGGTCTTAATCAC
GACGAAGCCCGGTTCAATGAGTTGCTCGTCGAGCAAGGCTCGTCACGCGCGGCGTTCGATGCCGAGGCGCGCACCGCCTC
AGAAAAGGACGTCAAGAGGCAGCTGTTGCTAGACGCCCTGGCCGATGAGCTGCAGGTCCAAGTTGGCCAGGATGATCTGA
CCGAACGACTGGTGACGACGTCTCGGCAATACGGCATCGAGCCGCAGCAGCTGTTCGGCTACCTCCAAGAGCGCAACCAG
CTGCCGACCATGTTCGCTGACGTGCGGCGCGAGCTGGCGATCAGGGCCGCAGTGGAGGCGGCGACGGTCACCGACAGTGA
CGGAAACACGATCGATACCAGTGAGTTCTTCGGCAAGCGTGTGTCGGCCGGTGAGGCTGAGGAGGCCGAACCGGCAGACG
AGGGTGCCGCGCGGGCGGCGTCCGACGAAGCGACAACGTGA

Upstream 100 bases:

>100_bases
AGCTTGGTAGCGCATCCGCTTTGGGAGCGGAAGGCCGCAGGTTCAAATCCTGTCACCCCGACCAGCCGACCGCCGTTATC
GACTACCAAGGAGCACACCC

Downstream 100 bases:

>100_bases
CCGTATGACGCTGTAAGCGAACGCGCCGGTTTCAGGGGGTGCGTGACCGCGAAACAGCCAGCCTGGTTGGTTAGTGTCGG
AGCATAGAAGAACTCGAGGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGREAMLDQIVNDALPSRYGQAV
AESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISPPDLSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVA
VGDVVSIDLSATVDGEDIPNAAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPESYVQAQFDSVLHSALSGLNH
DEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDALADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQ
LPTMFADVRRELAIRAAVEAATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT

Sequences:

>Translated_466_residues
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGREAMLDQIVNDALPSRYGQAV
AESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISPPDLSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVA
VGDVVSIDLSATVDGEDIPNAAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPESYVQAQFDSVLHSALSGLNH
DEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDALADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQ
LPTMFADVRRELAIRAAVEAATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT
>Mature_466_residues
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGREAMLDQIVNDALPSRYGQAV
AESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISPPDLSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVA
VGDVVSIDLSATVDGEDIPNAAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPESYVQAQFDSVLHSALSGLNH
DEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDALADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQ
LPTMFADVRRELAIRAAVEAATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=333, Percent_Identity=25.5255255255255, Blast_Score=128, Evalue=9e-31,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 50616; Mature: 50616

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGR
CCCCHHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCH
EAMLDQIVNDALPSRYGQAVAESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISPPD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHCCCCCEEEEEEECCCCCCCCC
LSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVAVGDVVSIDLSATVDGEDIPN
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCEECCEEEEEEEEEECCCCCCH
AAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
HHHHHHHHCCCCCCEEECCCCHHEECCCCCCEEEEEEECCCCCCCCCCEEEEEEEHHHHH
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SYVQAQFDSVLHSALSGLNHDEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
ADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQLPTMFADVRRELAIRAAVEA
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT
HEEECCCCCEECCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGR
CCCCHHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCH
EAMLDQIVNDALPSRYGQAVAESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISPPD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHCCCCCEEEEEEECCCCCCCCC
LSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVAVGDVVSIDLSATVDGEDIPN
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCEECCEEEEEEEEEECCCCCCH
AAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
HHHHHHHHCCCCCCEEECCCCHHEECCCCCCEEEEEEECCCCCCCCCCEEEEEEEHHHHH
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SYVQAQFDSVLHSALSGLNHDEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
ADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQLPTMFADVRRELAIRAAVEA
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT
HEEECCCCCEECCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]