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The map label for this gene is proB [H]

Identifier: 148823641

GI number: 148823641

Start: 2751791

End: 2752921

Strand: Reverse

Name: proB [H]

Synonym: TBFG_12466

Alternate gene names: 148823641

Gene position: 2752921-2751791 (Counterclockwise)

Preceding gene: 148823642

Following gene: 148823640

Centisome position: 62.22

GC content: 70.65

Gene sequence:

>1131_bases
ATGAGAAGTCCGCATCGGGACGCAATCCGGACCGCGCGCGGCCTTGTCGTGAAGGTCGGGACCACGGCGCTTACCACACC
GTCCGGGATGTTCGATGCCGGCCGGCTGGCCGGACTGGCCGAGGCGGTCGAGCGGCGGATGAAGGCGGGTTCCGACGTCG
TCATCGTGTCTTCGGGCGCCATCGCCGCCGGCATCGAGCCGCTCGGGCTGTCCCGTCGTCCCAAAGATCTGGCGACCAAG
CAGGCGGCGGCCAGCGTCGGGCAGGTCGCGCTGGTGAACTCGTGGAGCGCGGCGTTCGCCCGCTACGGCCGCACGGTGGG
CCAGGTGCTGCTGACCGCGCACGACATTTCGATGCGGGTGCAGCACACCAACGCCCAACGCACGCTGGATCGGCTGCGCG
CGTTGCACGCGGTGGCGATTGTCAACGAGAACGACACCGTGGCCACCAACGAGATCCGGTTCGGTGACAACGATCGGCTG
TCTGCACTGGTGGCGCACCTGGTCGGCGCCGACGCTTTGGTGCTGCTGTCGGACATCGACGGCCTCTACGACTGCGACCC
GCGCAAAACCGCGGACGCGACGTTCATTCCGGAGGTGTCCGGGCCGGCGGATCTGGACGGTGTGGTCGCCGGCCGCAGTA
GCCACCTGGGTACTGGCGGCATGGCGTCCAAGGTGGCGGCGGCGCTGTTGGCCGCCGACGCCGGGGTGCCGGTACTGCTG
GCCCCCGCGGCCGACGCCGCGACCGCGCTCGCCGACGCGTCGGTGGGCACGGTGTTTGCGGCCCGGCCCGCGCGTCTGTC
GGCCCGGCGGTTCTGGGTGCGTTATGCCGCCGAAGCAACCGGCGCACTGACTCTCGACGCCGGTGCGGTGCGCGCTGTGG
TGCGACAACGCCGGTCACTGCTGGCGGCGGGTATCACCGCGGTGTCCGGCCGGTTTTGCGGCGGCGATGTGGTCGAACTG
CGTGCACCCGACGCGGCCATGGTAGCCCGCGGGGTGGTTGCCTACGACGCGTCCGAGCTGGCCACCATGGTGGGCCGGTC
CACCTCTGAGCTACCCGGCGAGCTGCGCCGCCCGGTGGTGCACGCCGACGATCTGGTCGCGGTGTCGGCGAAGCAAGCTA
AGCAAGTTTAG

Upstream 100 bases:

>100_bases
CCGGCCGGGGCACCGATCCGCGGCTGGACAGCAACAAGCGGGTGGGCGCGGCCGAGCGAAAGGCCGCTCGGAGTCGGCGT
CGCGAACACGGGGATGGCTG

Downstream 100 bases:

>100_bases
CTACCATATTGCTCCGAGATGCGGTGTACCCCAAATGATTTCGATCAACATGGCCGCTAGCTGCGCATTGCGGCCGAGCG
GGTATCCGAGGTGAACTAGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGAIAAGIEPLGLSRRPKDLATK
QAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
SALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVAAALLAADAGVPVLL
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSLLAAGITAVSGRFCGGDVVEL
RAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVVHADDLVAVSAKQAKQV

Sequences:

>Translated_376_residues
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGAIAAGIEPLGLSRRPKDLATK
QAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
SALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVAAALLAADAGVPVLL
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSLLAAGITAVSGRFCGGDVVEL
RAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVVHADDLVAVSAKQAKQV
>Mature_376_residues
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGAIAAGIEPLGLSRRPKDLATK
QAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRL
SALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVAAALLAADAGVPVLL
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSLLAAGITAVSGRFCGGDVVEL
RAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVVHADDLVAVSAKQAKQV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=31.1036789297659, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=30.976430976431, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=40.5555555555556, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=34.6715328467153, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=34.6715328467153, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320506, Length=404, Percent_Identity=33.4158415841584, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=356, Percent_Identity=33.9887640449438, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=35.2941176470588, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 38789; Mature: 38789

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGA
CCCCHHHHHHHHCCEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
IAAGIEPLGLSRRPKDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRV
CEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEEEEE
QHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSDID
ECCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCCCCEEEEECCC
GLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVAAALLAADAGVPVLL
CCCCCCCCCCCCCEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSL
ECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
LAAGITAVSGRFCGGDVVELRAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHHCCEE
HADDLVAVSAKQAKQV
CCCCEEEECHHHHCCC
>Mature Secondary Structure
MRSPHRDAIRTARGLVVKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDVVIVSSGA
CCCCHHHHHHHHCCEEEEECCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
IAAGIEPLGLSRRPKDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRV
CEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEEEEE
QHTNAQRTLDRLRALHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSDID
ECCCHHHHHHHHHHHHHEEEECCCCCEEECEEEECCCHHHHHHHHHHHCCCCEEEEECCC
GLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSHLGTGGMASKVAAALLAADAGVPVLL
CCCCCCCCCCCCCEECCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
APAADAATALADASVGTVFAARPARLSARRFWVRYAAEATGALTLDAGAVRAVVRQRRSL
ECCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
LAAGITAVSGRFCGGDVVELRAPDAAMVARGVVAYDASELATMVGRSTSELPGELRRPVV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHCCHHHCCHHHHHHHHCCCCCCCCHHHHCCEE
HADDLVAVSAKQAKQV
CCCCEEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]