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The map label for this gene is nadE [H]

Identifier: 148823640

GI number: 148823640

Start: 2749466

End: 2751682

Strand: Reverse

Name: nadE [H]

Synonym: TBFG_12465

Alternate gene names: 148823640

Gene position: 2751682-2749466 (Counterclockwise)

Preceding gene: 148823641

Following gene: 148823637

Centisome position: 62.19

GC content: 63.96

Gene sequence:

>2217_bases
ATGGGACTGCTCGGCGGCCAATCAGGGCCCAGGGTCGGCAGCGGCCCAGTCGGTAGCATCCCCACGCCGGTCAATGCCGC
CATCTGCCAGCAGCGCGGGGGATTCCACGGTGTCGAGCGTGGATACTCGGCGGGTGATTCGGGCGTTCTGACGTCGCTGG
GCGACAATGAAAGGACGATGAACTTTTACTCCGCCTACCAGCACGGGTTCGTGCGCGTTGCCGCCTGCACTCACCACACC
ACCATCGGTGACCCGGCGGCCAACGCCGCGTCGGTATTGGACATGGCCCGTGCGTGCCACGACGATGGCGCAGCGTTGGC
GGTCTTTCCTGAGCTGACGCTGTCGGGCTACTCCATCGAGGACGTACTACTGCAGGACTCTCTGCTCGATGCCGTCGAGG
ACGCGCTGCTCGACCTGGTGACCGAATCCGCCGACCTGTTACCTGTACTGGTGGTCGGGGCTCCGCTGCGGCATCGACAC
CGCATCTACAACACCGCGGTCGTCATTCACCGCGGCGCCGTGCTCGGCGTGGTGCCCAAGTCGTATCTACCCACCTATCG
CGAGTTCTACGAGCTGCGCCAGATGGCGCCCGGAGACGGGGAGCGGGGCACGATCCGCATCGGTGGCGCCGACGTGGCCT
TCGGCACGGACCTGTTGTTCGCCGCGTCAGATCTACCCGGCTTTGTGTTGCATGTGGAGATCTGCGAGGACATGTTTGTG
CCGATGCCGCCCAGCGCCGAGGCGGCCCTGGCGGGCGCGACGGTGCTGGCGAATCTGTCCGGCAGCCCGATCACCATCGG
CCGTGCCGAGGACCGCCGGCTGCTTGCGCGCTCGGCGTCGGCGCGGTGTCTGGCTGCCTATGTCTATGCCGCCGCGGGGG
AGGGGGAGTCAACGACGGACCTGGCCTGGGACGGTCAGACGATGATCTGGGAGAATGGCGCACTGCTCGCGGAGTCCGAA
CGTTTCCCCAAAGGAGTGCGCCGCAGTGTCGCCGACGTTGACACCGAGTTGCTTCGGTCGGAGCGGCTGCGGATGGGCAC
GTTCGACGACAACCGGCGTCACCACCGGGAGTTAACGGAATCGTTCCGGCGCATCGACTTCGCACTCGACCCACCGGCAG
GCGACATCGGACTGCTGCGCGAGGTCGAGCGGTTCCCGTTCGTTCCGGCCGATCCGCAACGATTGCAACAGGATTGCTAC
GAGGCCTACAACATCCAGGTGTCTGGACTCGAGCAACGGTTGCGGGCGCTGGACTATCCGAAGGTCGTTATCGGTGTGTC
CGGGGGATTGGACTCGACGCACGCGCTGATCGTCGCGACCCATGCCATGGACCGCGAGGGCCGGCCGCGCAGCGACATTC
TGGCGTTTGCGTTGCCCGGATTCGCCACCGGGGAGCACACTAAGAACAACGCGATCAAGCTGGCACGTGCGCTGGGGGTT
ACCTTCTCCGAAATCGATATCGGCGACACCGCTCGGTTGATGCTGCACACAATCGGCCATCCGTATTCGGTTGGCGAAAA
AGTGTACGACGTCACCTTCGAGAACGTCCAGGCCGGGTTGCGCACCGACTATCTTTTCCGTATCGCCAACCAGCGCGGGG
GAATCGTACTGGGCACCGGGGACCTGTCGGAGCTGGCACTGGGTTGGTCGACATACGGTGTCGGCGACCAGATGTCGCAC
TACAACGTCAACGCCGGTGTGCCCAAGACGCTGATCCAGCACCTGATCCGGTGGGTCATTTCGGCGGGTGAGTTCGGTGA
GAAGGTGGGTGAGGTATTGCAGTCGGTGCTCGACACCGAGATCACCCCCGAACTCATTCCGACCGGCGAGGAGGAGCTGC
AGAGCAGCGAGGCCAAGGTCGGACCTTTCGCCCTACAGGACTTTTCGCTTTTTCAGGTACTGCGCTACGGATTTCGCCCG
TCGAAGATTGCGTTTTTGGCCTGGCATGCGTGGAACGATGCGGAGCGGGGCAACTGGCCGCCCGGCTTCCCAAAGAGCGA
ACGCCCGTCCTATTCATTGGCCGAAATCCGGCATTGGCTGCAGATTTTCGTCCAGCGGTTTTATTCGTTTAGCCAGTTCA
AGCGTTCGGCATTGCCCAACGGCCCCAAGGTGTCCCACGGGGGCGCGTTGTCGCCGCGTGGGGATTGGCGGGCCCCGTCG
GATATGTCAGCGCGAATCTGGCTCGATCAGATCGACCGTGAGGTGCCCAAGGGCTAG

Upstream 100 bases:

>100_bases
TTGCTCCGAGATGCGGTGTACCCCAAATGATTTCGATCAACATGGCCGCTAGCTGCGCATTGCGGCCGAGCGGGTATCCG
AGGTGAACTAGCGGATCGTC

Downstream 100 bases:

>100_bases
CGGATCAACCCCACACCCGGGACGAACCGGCCGACGCTGGCGGTGTAGCCGCGGTAGACGGCACTGTGCGTCCGCAACAG
GTAGGGTTCTTCGACGCGGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MGLLGGQSGPRVGSGPVGSIPTPVNAAICQQRGGFHGVERGYSAGDSGVLTSLGDNERTMNFYSAYQHGFVRVAACTHHT
TIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRH
RIYNTAVVIHRGAVLGVVPKSYLPTYREFYELRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFV
PMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMIWENGALLAESE
RFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCY
EAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALGV
TFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSH
YNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRP
SKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPS
DMSARIWLDQIDREVPKG

Sequences:

>Translated_738_residues
MGLLGGQSGPRVGSGPVGSIPTPVNAAICQQRGGFHGVERGYSAGDSGVLTSLGDNERTMNFYSAYQHGFVRVAACTHHT
TIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRH
RIYNTAVVIHRGAVLGVVPKSYLPTYREFYELRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFV
PMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMIWENGALLAESE
RFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCY
EAYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALGV
TFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSH
YNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRP
SKIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPS
DMSARIWLDQIDREVPKG
>Mature_737_residues
GLLGGQSGPRVGSGPVGSIPTPVNAAICQQRGGFHGVERGYSAGDSGVLTSLGDNERTMNFYSAYQHGFVRVAACTHHTT
IGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRHR
IYNTAVVIHRGAVLGVVPKSYLPTYREFYELRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFVP
MPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMIWENGALLAESER
FPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYE
AYNIQVSGLEQRLRALDYPKVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALGVT
FSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSHY
NVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRPS
KIAFLAWHAWNDAERGNWPPGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPSD
MSARIWLDQIDREVPKG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=625, Percent_Identity=23.36, Blast_Score=85, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321865, Length=654, Percent_Identity=23.7003058103976, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24641841, Length=634, Percent_Identity=23.6593059936909, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641843, Length=634, Percent_Identity=23.6593059936909, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 80449; Mature: 80318

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00591 GLYCOSYL_HYDROL_F10 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLGGQSGPRVGSGPVGSIPTPVNAAICQQRGGFHGVERGYSAGDSGVLTSLGDNERTM
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCCCEECCCCCCHHH
NFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIE
HHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEECCCCHH
DVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPK
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEEEEECCCEEEECCH
SYLPTYREFYELRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFV
HHCCHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCHHHEEECCCCCEEEEEEEHHHCCC
PMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTD
CCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCE
LAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTE
EEECCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYP
HHHHCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHCCCC
KVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALGV
EEEEEECCCCCCCCEEEEEEEHHCCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHCC
TFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTG
EEEEECCCCHHHHHHHHCCCCHHHCCHHEEEEHHHHHHCCCHHHHHEECCCCCCEEEECC
DLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTE
CHHHHHHCCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
ITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNWP
CCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
PGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
DMSARIWLDQIDREVPKG
CCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLLGGQSGPRVGSGPVGSIPTPVNAAICQQRGGFHGVERGYSAGDSGVLTSLGDNERTM
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCCCEECCCCCCHHH
NFYSAYQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIE
HHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCEECCCCHH
DVLLQDSLLDAVEDALLDLVTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPK
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEEEEECCCEEEECCH
SYLPTYREFYELRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFV
HHCCHHHHHHHHHHCCCCCCCCCEEEECCCCEECCCHHHEEECCCCCEEEEEEEHHHCCC
PMPPSAEAALAGATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTD
CCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCE
LAWDGQTMIWENGALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTE
EEECCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYP
HHHHCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECHHHHHHHCCCC
KVVIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALGV
EEEEEECCCCCCCCEEEEEEEHHCCCCCCHHHEEEEECCCCCCCCCCCCHHHHHHHHHCC
TFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTG
EEEEECCCCHHHHHHHHCCCCHHHCCHHEEEEHHHHHHCCCHHHHHEECCCCCCEEEECC
DLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTE
CHHHHHHCCHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
ITPELIPTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNWP
CCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCC
PGFPKSERPSYSLAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
DMSARIWLDQIDREVPKG
CCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]