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The map label for this gene is mez

Identifier: 148823533

GI number: 148823533

Start: 2615905

End: 2617551

Strand: Direct

Name: mez

Synonym: TBFG_12356

Alternate gene names: 148823533

Gene position: 2615905-2617551 (Clockwise)

Preceding gene: 148823532

Following gene: 148823535

Centisome position: 59.12

GC content: 63.45

Gene sequence:

>1647_bases
ATGAGCGACGCCCGCGTGCCACGGATCCCGGCCGCGTTGTCCGCACCAAGTCTCAACCGTGGAGTCGGCTTCACCCACGC
GCAGCGGCGGCGGCTGGGGCTGACCGGCCGGCTTCCGTCGGCCGTGCTCACGCTCGACCAACAGGCCGAACGCGTATGGC
ATCAGTTGCAGAGCTTGGCCACCGAGCTGGGCCGCAACCTGCTTCTCGAACAGCTGCACTACCGCCACGAGGTGCTGTAC
TTCAAGGTGCTGGCCGACCATTTGCCCGAACTGATGCCGGTGGTGTACACGCCCACCGTTGGCGAGGCAATCCAACGCTT
CTCCGACGAATACCGCGGGCAACGCGGACTGTTTCTGAGCATCGACGAACCCGACGAAATCGAGGAAGCCTTCAACACGT
TGGGGCTGGGGCCCGAGGACGTCGACCTGATCGTGTGCACCGATGCCGAGGCGATCCTGGGTATCGGTGACTGGGGTGTG
GGTGGCATCCAGATCGCTGTGGGCAAATTGGCCCTCTACACCGCCGGCGGCGGCGTCGATCCGCGCCGCTGCCTCGCGGT
GTCTCTGGATGTCGGCACCGACAATGAGCAGCTGCTGGCCGATCCGTTCTATCTGGGCAATCGCCACGCCCGGCGGCGCG
GTCGGGAATACGACGAGTTCGTCAGTCGCTATATCGAAACGGCTCAACGGTTATTTCCGCGTGCCATTCTGCATTTCGAG
GACTTCGGGCCGGCGAACGCGCGGAAGATCCTAGACACATACGGCACGGATTACTGCGTGTTCAACGATGACATGCAAGG
AACCGGCGCGGTGGTCTTGGCCGCCGTATACAGCGGTCTGAAGGTTACCGGTATCCCGCTGCGCGATCAGACAATAGTCG
TCTTCGGCGCAGGCACCGCAGGGATGGGGATCGCCGATCAGATCCGGGACGCGATGGTGGCAGACGGTGCCACGCTCGAG
CAGGCGGTGTCCCAGATCTGGCCGATCGACAGGCCGGGCCTGTTGTTCGACGACATGGATGACCTGCGCGACTTCCAAGT
GCCGTACGCGAAAAACCGCCACCAGCTCGGTGTGGCCGTCGGGGATCGGGTCGGGCTGAGCGACGCGATCAAGATCGCAT
CGCCCACTATCCTGCTCGGCTGCTCAACGGTCTACGGAGCGTTCACCAAAGAGGTGGTCGAGGCGATGACGGCGTCCTGC
AAACACCCGATGATCTTTCCGCTGTCCAACCCGACGTCGCGCATGGAAGCCATCCCCGCCGACGTGCTGGCGTGGTCGAA
TGGCAGGGCGCTGCTTGCCACCGGCAGCCCAGTCGCCCCAGTGGAATTCGACGAAACCACCTACGTCATCGGTCAGGCCA
ACAACGTGTTGGCGTTTCCCGGCATCGGACTGGGCGTCATTGTCGCTGGTGCCCGGTTGATAACCAGGCGCATGCTGCAT
GCAGCAGCGAAGGCCATTGCGCACCAGGCCAATCCGACAAATCCCGGAGACTCGCTGTTGCCGGATGTCCAAAATCTGCG
GGCCATCTCGACAACGGTCGCCGAAGCTGTCTATCGGGCCGCCGTCCAAGACGGGGTGGCTTCCAGGACGCACGACGACG
TCAGGCAGGCCATAGTCGACACCATGTGGCTCCCGGCATATGACTAA

Upstream 100 bases:

>100_bases
ACCTGGACCTGGCCAAAGCCCAAGTCCGGTCGGCGCTCGCCAACCAGCGTTACCATCGCGACGTCCATTAAACCCAGCAC
GGTCACGAACGGAGGTTGTG

Downstream 100 bases:

>100_bases
CCGCGCACTCGACGGTCATCGCTGTAGGCAGCCTCTCGCTTAGGTCGCTGCCCGCGGTGTGCACGTCACGCGGAAACCAT
CGCCAGCCGGCGAGAAACAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: Malic enzyme

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAERVWHQLQSLATELGRNLLLEQLHYRHEVLY
FKVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGV
GGIQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFE
DFGPANARKILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLE
QAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASC
KHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLH
AAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAAVQDGVASRTHDDVRQAIVDTMWLPAYD

Sequences:

>Translated_548_residues
MSDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAERVWHQLQSLATELGRNLLLEQLHYRHEVLY
FKVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGV
GGIQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFE
DFGPANARKILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLE
QAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASC
KHPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLH
AAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAAVQDGVASRTHDDVRQAIVDTMWLPAYD
>Mature_547_residues
SDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAERVWHQLQSLATELGRNLLLEQLHYRHEVLYF
KVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLSIDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGVG
GIQIAVGKLALYTAGGGVDPRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFED
FGPANARKILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTAGMGIADQIRDAMVADGATLEQ
AVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAVGDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCK
HPMIFPLSNPTSRMEAIPADVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLHA
AAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAAVQDGVASRTHDDVRQAIVDTMWLPAYD

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family

Homologues:

Organism=Homo sapiens, GI4505143, Length=517, Percent_Identity=38.6847195357834, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI62420882, Length=539, Percent_Identity=35.621521335807, Blast_Score=348, Evalue=1e-95,
Organism=Homo sapiens, GI62420880, Length=539, Percent_Identity=35.621521335807, Blast_Score=348, Evalue=1e-95,
Organism=Homo sapiens, GI239049447, Length=539, Percent_Identity=35.621521335807, Blast_Score=348, Evalue=1e-95,
Organism=Homo sapiens, GI4505145, Length=540, Percent_Identity=36.2962962962963, Blast_Score=335, Evalue=9e-92,
Organism=Homo sapiens, GI270265879, Length=450, Percent_Identity=38.6666666666667, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI87081919, Length=542, Percent_Identity=41.3284132841328, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17537199, Length=543, Percent_Identity=38.4898710865562, Blast_Score=352, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6322823, Length=557, Percent_Identity=42.7289048473968, Blast_Score=443, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21356279, Length=518, Percent_Identity=38.8030888030888, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281362674, Length=518, Percent_Identity=38.8030888030888, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281362672, Length=518, Percent_Identity=38.8030888030888, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24646386, Length=515, Percent_Identity=36.8932038834951, Blast_Score=351, Evalue=9e-97,
Organism=Drosophila melanogaster, GI24646388, Length=515, Percent_Identity=36.8932038834951, Blast_Score=351, Evalue=9e-97,
Organism=Drosophila melanogaster, GI78707232, Length=517, Percent_Identity=33.8491295938104, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI281363505, Length=534, Percent_Identity=32.7715355805243, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI78707236, Length=534, Percent_Identity=32.7715355805243, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI281363503, Length=532, Percent_Identity=32.7067669172932, Blast_Score=308, Evalue=5e-84,
Organism=Drosophila melanogaster, GI78707242, Length=545, Percent_Identity=32.1100917431193, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI78707238, Length=545, Percent_Identity=32.1100917431193, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI78707240, Length=527, Percent_Identity=32.258064516129, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI19922384, Length=539, Percent_Identity=26.9016697588126, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAOX_MYCTU (P71880)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   E70705
- RefSeq:   NP_216848.2
- RefSeq:   NP_336872.1
- ProteinModelPortal:   P71880
- SMR:   P71880
- EnsemblBacteria:   EBMYCT00000002332
- EnsemblBacteria:   EBMYCT00000069852
- GeneID:   887962
- GeneID:   924018
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2394
- KEGG:   mtu:Rv2332
- TIGR:   MT2394
- TubercuList:   Rv2332
- GeneTree:   EBGT00050000016971
- HOGENOM:   HBG289821
- OMA:   FTHEQRR
- ProtClustDB:   PRK13529
- BRENDA:   1.1.1.38
- GO:   GO:0005829
- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000106
- PRINTS:   PR00072
- SMART:   SM00919

Pfam domain/function: PF00390 malic; PF03949 Malic_M

EC number: =1.1.1.38

Molecular weight: Translated: 59423; Mature: 59292

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: ACT_SITE 96-96 ACT_SITE 169-169 BINDING 264-264 BINDING 410-410

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAERVWHQLQSLA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TELGRNLLLEQLHYRHEVLYFKVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHCCCCCEEEE
IDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGVGGIQIAVGKLALYTAGGGVD
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCCCCCEEEEEEEEEEEECCCCCC
PRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFE
CCCEEEEEEECCCCCCHHEECCHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DFGPANARKILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTA
CCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEEEECCCC
GMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEE
GDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCKHPMIFPLSNPTSRMEAIPA
CCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCH
DVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLH
HHEEECCCCEEEECCCCCCCEEECCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHH
AAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAAVQDGVASRTHDDVRQAIVD
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TMWLPAYD
HHCCCCCC
>Mature Secondary Structure 
SDARVPRIPAALSAPSLNRGVGFTHAQRRRLGLTGRLPSAVLTLDQQAERVWHQLQSLA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TELGRNLLLEQLHYRHEVLYFKVLADHLPELMPVVYTPTVGEAIQRFSDEYRGQRGLFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHCCCCCEEEE
IDEPDEIEEAFNTLGLGPEDVDLIVCTDAEAILGIGDWGVGGIQIAVGKLALYTAGGGVD
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCCCCCEEEEEEEEEEEECCCCCC
PRRCLAVSLDVGTDNEQLLADPFYLGNRHARRRGREYDEFVSRYIETAQRLFPRAILHFE
CCCEEEEEEECCCCCCHHEECCHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DFGPANARKILDTYGTDYCVFNDDMQGTGAVVLAAVYSGLKVTGIPLRDQTIVVFGAGTA
CCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEEEECCCC
GMGIADQIRDAMVADGATLEQAVSQIWPIDRPGLLFDDMDDLRDFQVPYAKNRHQLGVAV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEE
GDRVGLSDAIKIASPTILLGCSTVYGAFTKEVVEAMTASCKHPMIFPLSNPTSRMEAIPA
CCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCH
DVLAWSNGRALLATGSPVAPVEFDETTYVIGQANNVLAFPGIGLGVIVAGARLITRRMLH
HHEEECCCCEEEECCCCCCCEEECCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHH
AAAKAIAHQANPTNPGDSLLPDVQNLRAISTTVAEAVYRAAVQDGVASRTHDDVRQAIVD
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
TMWLPAYD
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036