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The map label for this gene is pitB [C]

Identifier: 148823483

GI number: 148823483

Start: 2563961

End: 2565619

Strand: Direct

Name: pitB [C]

Synonym: TBFG_12304

Alternate gene names: 148823483

Gene position: 2563961-2565619 (Clockwise)

Preceding gene: 148823482

Following gene: 148823485

Centisome position: 57.95

GC content: 62.09

Gene sequence:

>1659_bases
ATGAGCGACAATGCCAAACACCACAGGGATGGGCATTTGGTGGCTAGCGGACTTCAGGATCGCGCAGCGCGCACACCGCA
ACACGAGGGCTTCCTCGGGCCGGACCGACCATGGCACCTGTCGTTCAGTCTGCTGCTGGCGGGTTCTTTCGTGCTGTTCT
CGTGGTGGGCATTCGACTACGCAGGGTCCGGCGCGAACAAAGTCATCCTGGTGCTCGCCACCGTCGTCGGCATGTTCATG
GCCTTCAACGTCGGCGGCAATGATGTCGCCAACTCGTTTGGCACCAGCGTCGGCGCGGGCACGTTGACCATGAAACAGGC
GCTTCTGGTCGCGGCGATCTTCGAGGTCAGCGGCGCGGTGATCGCCGGCGGCGACGTCACCGAGACCATCCGCAGCGGCA
TCGTTGATCTGTCCGGGGTGTCCGTCGACCCACGCGACTTCATGAACATCATGCTGTCGGCGCTATCGGCAGCCGCGCTC
TGGCTGCTGTTTGCTAACCGTATGGGGTACCCGGTGTCGACCACACACTCGATCATCGGCGGCATCGTCGGCGCGGCGAT
CGCGCTGGGGATGGTGAGCGGCCAGGGCGGTGCCGCACTCAGGATGGTCCAGTGGGATCAAATCGGCCAGATCGTGGTGT
CCTGGGTGCTGTCGCCGGTGTTGGGCGGCTTGGTGTCGTACCTGCTCTACGGCGTCATCAAACGGCACATCCTGCTGTAC
AACGAACAGGCCGAACGACGGCTAACAGAAATTAAGAAAGAGCGCATCGCACACCGCGAGCGCCACAAGGCGGCGTTCGA
CCGGCTCACCGAGATCCAGCAGATCGCCTATACCGGCGCCCTGGCGCGCGACGCCGTCGCGGCAAACCGCAAGGACTTTG
ATCCCGACGAACTGGAATCCGATTACTACCGCGAGCTACACGAAATCGACGCCAAGACATCGTCGGTCGACGCGTTCCGG
GCCCTGCAGAACTGGGTTCCGCTGGTCGCCGCCGCCGGATCCATGATCATTGTCGCGATGCTGCTGTTCAAGGGGTTCAA
GCACATGCACTTGGGCCTTACCACGATGAATAACTACTTCATCATCGCGATGGTCGGTGCAGCGGTGTGGATGGCCACCT
TTATTTTCGCCAAGACACTTCGGGGCGAATCACTTTCACGGTCAACGTTTTTGATGTTCAGCTGGATGCAGGTCTTTACG
GCCTCGGGCTTCGCCTTCAGCCACGGCAGCAATGACATTGCCAACGCCATCGGGCCGTTCGCGGCAATCCTGGATGTGCT
GCGCACGGGCGCCATTGAAGGCAACGCAGCGGTGCCTGCCGCGGCCATGGTAACGTTCGGCGTCGCGTTGTGCGCGGGGT
TGTGGTTCATTGGACGACGGGTGATCGCCACCGTTGGACACAACCTCACCACGATGCACCCGGCATCGGGGTTTGCTGCC
GAATTGTCGGCCGCCGGGGTGGTCATGGGAGCCACGGTCCTGGGTCTTCCGGTTTCCAGCACGCACATTCTTATCGGCGC
CGTCCTCGGCGTCGGCATCGTAAACCGGTCCACCAACTGGGGACTGATGAAACCGATCGTGCTAGCGTGGGTCATCACGC
TGCCTTCGGCGGCGATCCTCGCCTCGGTCGGTCTTGTCGCGCTACGCGCGATTTTCTGA

Upstream 100 bases:

>100_bases
AGTCCGATGCAGCGTCGGTCACCGTTATTCATCCGGCGTTTACCCGTTGCTAGCCGCCATGACGTAGCCTGCTGACGCTC
GATCGCCAACACAAGCCGAC

Downstream 100 bases:

>100_bases
CGACGCCGGGTCCATCAACCCCAGCGCAACCTCCGCGAGCAGTCGCTAAAGCCCCCGACACGCCGTGCGTGCGGGGGCTT
ATGCGACTGCTCGCCGGACG

Product: phosphate-transport system permease pitB

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSDNAKHHRDGHLVASGLQDRAARTPQHEGFLGPDRPWHLSFSLLLAGSFVLFSWWAFDYAGSGANKVILVLATVVGMFM
AFNVGGNDVANSFGTSVGAGTLTMKQALLVAAIFEVSGAVIAGGDVTETIRSGIVDLSGVSVDPRDFMNIMLSALSAAAL
WLLFANRMGYPVSTTHSIIGGIVGAAIALGMVSGQGGAALRMVQWDQIGQIVVSWVLSPVLGGLVSYLLYGVIKRHILLY
NEQAERRLTEIKKERIAHRERHKAAFDRLTEIQQIAYTGALARDAVAANRKDFDPDELESDYYRELHEIDAKTSSVDAFR
ALQNWVPLVAAAGSMIIVAMLLFKGFKHMHLGLTTMNNYFIIAMVGAAVWMATFIFAKTLRGESLSRSTFLMFSWMQVFT
ASGFAFSHGSNDIANAIGPFAAILDVLRTGAIEGNAAVPAAAMVTFGVALCAGLWFIGRRVIATVGHNLTTMHPASGFAA
ELSAAGVVMGATVLGLPVSSTHILIGAVLGVGIVNRSTNWGLMKPIVLAWVITLPSAAILASVGLVALRAIF

Sequences:

>Translated_552_residues
MSDNAKHHRDGHLVASGLQDRAARTPQHEGFLGPDRPWHLSFSLLLAGSFVLFSWWAFDYAGSGANKVILVLATVVGMFM
AFNVGGNDVANSFGTSVGAGTLTMKQALLVAAIFEVSGAVIAGGDVTETIRSGIVDLSGVSVDPRDFMNIMLSALSAAAL
WLLFANRMGYPVSTTHSIIGGIVGAAIALGMVSGQGGAALRMVQWDQIGQIVVSWVLSPVLGGLVSYLLYGVIKRHILLY
NEQAERRLTEIKKERIAHRERHKAAFDRLTEIQQIAYTGALARDAVAANRKDFDPDELESDYYRELHEIDAKTSSVDAFR
ALQNWVPLVAAAGSMIIVAMLLFKGFKHMHLGLTTMNNYFIIAMVGAAVWMATFIFAKTLRGESLSRSTFLMFSWMQVFT
ASGFAFSHGSNDIANAIGPFAAILDVLRTGAIEGNAAVPAAAMVTFGVALCAGLWFIGRRVIATVGHNLTTMHPASGFAA
ELSAAGVVMGATVLGLPVSSTHILIGAVLGVGIVNRSTNWGLMKPIVLAWVITLPSAAILASVGLVALRAIF
>Mature_551_residues
SDNAKHHRDGHLVASGLQDRAARTPQHEGFLGPDRPWHLSFSLLLAGSFVLFSWWAFDYAGSGANKVILVLATVVGMFMA
FNVGGNDVANSFGTSVGAGTLTMKQALLVAAIFEVSGAVIAGGDVTETIRSGIVDLSGVSVDPRDFMNIMLSALSAAALW
LLFANRMGYPVSTTHSIIGGIVGAAIALGMVSGQGGAALRMVQWDQIGQIVVSWVLSPVLGGLVSYLLYGVIKRHILLYN
EQAERRLTEIKKERIAHRERHKAAFDRLTEIQQIAYTGALARDAVAANRKDFDPDELESDYYRELHEIDAKTSSVDAFRA
LQNWVPLVAAAGSMIIVAMLLFKGFKHMHLGLTTMNNYFIIAMVGAAVWMATFIFAKTLRGESLSRSTFLMFSWMQVFTA
SGFAFSHGSNDIANAIGPFAAILDVLRTGAIEGNAAVPAAAMVTFGVALCAGLWFIGRRVIATVGHNLTTMHPASGFAAE
LSAAGVVMGATVLGLPVSSTHILIGAVLGVGIVNRSTNWGLMKPIVLAWVITLPSAAILASVGLVALRAIF

Specific function: Potential transporter for phosphate

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family

Homologues:

Organism=Homo sapiens, GI31543630, Length=166, Percent_Identity=42.1686746987952, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=41.5584415584416, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17539280, Length=517, Percent_Identity=32.1083172147002, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71981576, Length=159, Percent_Identity=42.1383647798742, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25146401, Length=164, Percent_Identity=42.6829268292683, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32566716, Length=169, Percent_Identity=39.6449704142012, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17536725, Length=164, Percent_Identity=30.4878048780488, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17557328, Length=157, Percent_Identity=31.8471337579618, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=155, Percent_Identity=35.4838709677419, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21356511, Length=174, Percent_Identity=41.3793103448276, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2281_MYCTU (P65712)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   E70731
- RefSeq:   NP_216797.1
- RefSeq:   NP_336809.1
- ProteinModelPortal:   P65712
- EnsemblBacteria:   EBMYCT00000002068
- EnsemblBacteria:   EBMYCT00000072708
- GeneID:   887257
- GeneID:   924084
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2339
- KEGG:   mtu:Rv2281
- TIGR:   MT2339
- TubercuList:   Rv2281
- GeneTree:   EBGT00050000017317
- HOGENOM:   HBG681698
- OMA:   KLNPMRA
- ProtClustDB:   CLSK872042
- InterPro:   IPR001204
- PANTHER:   PTHR11101

Pfam domain/function: PF01384 PHO4

EC number: NA

Molecular weight: Translated: 58790; Mature: 58659

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1a80d2f4)-; HASH(0x1b2dcfdc)-; HASH(0x1ba22e3c)-; HASH(0x1ac68470)-; HASH(0x1b003acc)-; HASH(0x1b4cb960)-; HASH(0x1bd71c20)-; HASH(0x1b31d1d4)-; HASH(0x1b946298)-; HASH(0x1a312d24)-; HASH(0x1ad13d74)-; HASH(0x1bc09cd4)-; HASH(0x174d1344)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCH
>Mature Secondary Structure 
SDNAKHHRDGHLVASGLQDRAARTPQHEGFLGPDRPWHLSFSLLLAGSFVLFSWWAFDY
CCCCCCCCCCCHHHHCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGSGANKVILVLATVVGMFMAFNVGGNDVANSFGTSVGAGTLTMKQALLVAAIFEVSGAV
CCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCE
IAGGDVTETIRSGIVDLSGVSVDPRDFMNIMLSALSAAALWLLFANRMGYPVSTTHSIIG
EECCCHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GIVGAAIALGMVSGQGGAALRMVQWDQIGQIVVSWVLSPVLGGLVSYLLYGVIKRHILLY
HHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEQAERRLTEIKKERIAHRERHKAAFDRLTEIQQIAYTGALARDAVAANRKDFDPDELES
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
DYYRELHEIDAKTSSVDAFRALQNWVPLVAAAGSMIIVAMLLFKGFKHMHLGLTTMNNYF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEECCCEE
IIAMVGAAVWMATFIFAKTLRGESLSRSTFLMFSWMQVFTASGFAFSHGSNDIANAIGPF
EHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
AAILDVLRTGAIEGNAAVPAAAMVTFGVALCAGLWFIGRRVIATVGHNLTTMHPASGFAA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHH
ELSAAGVVMGATVLGLPVSSTHILIGAVLGVGIVNRSTNWGLMKPIVLAWVITLPSAAIL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHH
ASVGLVALRAIF
HHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036