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The map label for this gene is cobD [H]

Identifier: 148823444

GI number: 148823444

Start: 2520580

End: 2521521

Strand: Reverse

Name: cobD [H]

Synonym: TBFG_12265

Alternate gene names: 148823444

Gene position: 2521521-2520580 (Counterclockwise)

Preceding gene: 148823446

Following gene: 148823440

Centisome position: 56.99

GC content: 71.97

Gene sequence:

>942_bases
GTGTTTGCATCGACCTGGCAGACCAGGGCCGTCGGCGTGCTGATCGGCTGCCTGCTCGACGTCGTGTTCGGCGACCCCAA
ACGAGGTCATCCGGTGGCCCTGTTCGGTCGGGCGGCCGCCAAGCTGGAGCAGATCACCTACCGTGACGGCCGGGTCGCCG
GTGCGGTACACGTCGGCCTGCTGGTCGGCGCGGTGGGCTTGCTCGGCGCGGCACTGCAGCGGCTACCCGGCAGGTGCTGG
CCCGTGGCGGCCACCGCGACGGCCACCTGGGCAGCGCTGGGCGGAACTTCGCTGGCGCGCACCGGCCGCCAGATATCGGA
CCTGTTGGAGCGCGACGATGTCGAGGCGGCGCGACGGCTGCTGCCGTCGCTGTGCGGGCGTGACCCGGCCCAGCTGGGCG
GCCCGGGCCTGACGCGTGCCGCGCTGGAGTCGGTGGCCGAAAACACCGCCGACGCCCAGGTGGTGCCGCTGCTGTGGGCG
GCTTCGAGCGGCGTGCCTGCGGTGCTGGGATATCGTGCCATCAACACCCTGGACTCGATGATCGGCTACCGCTCGCCGCG
TTATCTCCGATTCGGTTGGGCTGCAGCACGATTGGATGACTGGGCCAACTATGTTGGCGCACGGGCGACGGCGGTGCTGG
TGGTGATCTGCGCGCCGGTGGTCGGTGGGTCGCCCCGCGGTGCGGTACGGGCCTGGCGGCGCGACGCCGCCCGCCATCCC
AGCCCCAACGCCGGTGTCGTCGAGGCGGCGTTTGCTGGGGCGCTTGACGTGCGGCTGGGTGGGCCCACCCGGTACCACCA
TGAGTTGCAGATCCGGCCCACCTTGGGCGACGGCCGGTCGCCCAAGGTGGCCGATCTGCGCCGCGCCGTGGTGCTGTCGC
GGGTGGTCCAGGCGGGGGCTGCGGTATTGGCCGTGATGTTGGTTTACCGCCGGCGGCCGTAG

Upstream 100 bases:

>100_bases
AACAGGTCGGCAACCCGGGTAGCTGGCCGCTTCATCGTGGATAGCTGCCGTATGAGGTCATAAATAGCTGAGCGTAGACC
ACGTGTGCGACGCTGCCGGA

Downstream 100 bases:

>100_bases
CGGTCAGCGAGTTTCTGCTCGACCGTCATTGGCTTGTCCGGTGGTGGCGACCCCGCTTTTTCCCGGCGGCGCGCCCGGAT
CTCGGCGTAGGCGAAATAGC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MFASTWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRCW
PVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWA
ASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP

Sequences:

>Translated_313_residues
MFASTWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRCW
PVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWA
ASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP
>Mature_313_residues
MFASTWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGLLVGAVGLLGAALQRLPGRCW
PVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRLLPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWA
ASSGVPAVLGYRAINTLDSMIGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGAAVLAVMLVYRRRP

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 32993; Mature: 32993

Theoretical pI: Translated: 11.31; Mature: 11.31

Prosite motif: PS00139 THIOL_PROTEASE_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASTWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHH
LVGAVGLLGAALQRLPGRCWPVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRL
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
LPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWAASSGVPAVLGYRAINTLDSM
HHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHH
IGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGA
CCCCCHHHHHHHCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AVLAVMLVYRRRP
HHHHHHHHHHCCC
>Mature Secondary Structure
MFASTWQTRAVGVLIGCLLDVVFGDPKRGHPVALFGRAAAKLEQITYRDGRVAGAVHVGL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHH
LVGAVGLLGAALQRLPGRCWPVAATATATWAALGGTSLARTGRQISDLLERDDVEAARRL
HHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHH
LPSLCGRDPAQLGGPGLTRAALESVAENTADAQVVPLLWAASSGVPAVLGYRAINTLDSM
HHHHHCCCCHHHCCCCHHHHHHHHHHHCCCCCEEHHHEEECCCCCCHHHHHHHHHHHHHH
IGYRSPRYLRFGWAAARLDDWANYVGARATAVLVVICAPVVGGSPRGAVRAWRRDAARHP
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SPNAGVVEAAFAGALDVRLGGPTRYHHELQIRPTLGDGRSPKVADLRRAVVLSRVVQAGA
CCCCCHHHHHHHCEEEEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AVLAVMLVYRRRP
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]