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The map label for this gene is ilvE

Identifier: 148823417

GI number: 148823417

Start: 2487010

End: 2488116

Strand: Reverse

Name: ilvE

Synonym: TBFG_12238

Alternate gene names: 148823417

Gene position: 2488116-2487010 (Counterclockwise)

Preceding gene: 148823418

Following gene: 148823412

Centisome position: 56.24

GC content: 65.4

Gene sequence:

>1107_bases
ATGACCAGCGGCTCCCTTCAATTCACGGTGTTACGTGCGGTCAATCCGGCCACCGACGCGCAGCGTGAATCGATGCTGCG
GGAGCCGGGTTTCGGCAAATACCACACCGACCATATGGTGTCGATCGACTATGCCGAGGGCCGTGGTTGGCACAACGCGC
GGGTAATCCCTTATGGCCCGATCGAGCTGGATCCCTCGGCGATCGTGCTGCACTATGCGCAGGAGGTGTTCGAAGGGCTC
AAAGCCTACCGCTGGGCCGACGGGTCCATCGTGTCGTTTCGCGCCGACGCCAACGCCGCCAGGTTGCGTTCGTCGGCGCG
GCGGTTGGCGATTCCCGAACTGCCCGACGCGGTGTTCATCGAATCCCTGCGCCAGCTAATCGCTGTCGACAAAGCTTGGG
TGCCCGGTGCCGGCGGTGAGGAGGCGCTGTATCTGCGGCCGTTCATCTTCGCCACCGAGCCGGGACTGGGCGTGCGGCCT
GCCACCCAATACCGTTACCTGTTGATCGCCTCGCCGGCCGGTGCGTACTTCAAGGGCGGCATCGCCCCTGTCAGCGTCTG
GGTTTCGACGGAGTATGTACGGGCCTGTCCGGGCGGCACCGGTGCGGCCAAGTTCGGCGGCAACTACGCCGCCTCGTTGC
TGGCGCAGGCCGAAGCCGCCGAGAACGGATGCGACCAGGTGGTGTGGCTGGACGCTGTGGAACGCCGCTATATCGAAGAG
ATGGGTGGCATGAACATCTTCTTCGTGCTCGGCAGCGGCGGATCGGCGCGGCTGGTCACCCCGGAGCTGTCCGGTTCCCT
GCTGCCCGGGATCACACGGGATTCGTTGTTGCAGTTGGCTATTGATGCCGGATTCGCGGTCGAGGAACGCAGGATTGATA
TCGACGAGTGGCAGAAGAAAGCCGCCGCCGGCGAGATCACCGAGGTGTTTGCGTGCGGCACCGCCGCTGTCATCACCCCG
GTCGCGCGGGTGCGGCACGGTGCCAGCGAGTTCAGAATCGCCGACGGTCAGCCGGGTGAGGTGACCATGGCACTACGCGA
TACGCTGACCGGCATCCAGCGGGGCACCTTCGCGGACACCCACGGCTGGATGGCGCGGCTGGGGTAG

Upstream 100 bases:

>100_bases
CGCCGTTCGTTGCCGTGAAAACGCGCTGAGTCACCACTTGGTGGCAGCGGAGTGGCCTCCGGGCCGTCAATAACCGTTCG
CCCACGGATAGAATCGGCCC

Downstream 100 bases:

>100_bases
TGCGGCAGCGGGTTTGCCGGGCGCTGGACGGCGGGCCGCGCGGCGACAGCTTGGCCGGTTCAGGGGTGGCGCGGGCCGGC
GACTGTCCCATACGTGTCGT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTSGSLQFTVLRAVNPATDAQRESMLREPGFGKYHTDHMVSIDYAEGRGWHNARVIPYGPIELDPSAIVLHYAQEVFEGL
KAYRWADGSIVSFRADANAARLRSSARRLAIPELPDAVFIESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRP
ATQYRYLLIASPAGAYFKGGIAPVSVWVSTEYVRACPGGTGAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEE
MGGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERRIDIDEWQKKAAAGEITEVFACGTAAVITP
VARVRHGASEFRIADGQPGEVTMALRDTLTGIQRGTFADTHGWMARLG

Sequences:

>Translated_368_residues
MTSGSLQFTVLRAVNPATDAQRESMLREPGFGKYHTDHMVSIDYAEGRGWHNARVIPYGPIELDPSAIVLHYAQEVFEGL
KAYRWADGSIVSFRADANAARLRSSARRLAIPELPDAVFIESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRP
ATQYRYLLIASPAGAYFKGGIAPVSVWVSTEYVRACPGGTGAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEE
MGGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERRIDIDEWQKKAAAGEITEVFACGTAAVITP
VARVRHGASEFRIADGQPGEVTMALRDTLTGIQRGTFADTHGWMARLG
>Mature_367_residues
TSGSLQFTVLRAVNPATDAQRESMLREPGFGKYHTDHMVSIDYAEGRGWHNARVIPYGPIELDPSAIVLHYAQEVFEGLK
AYRWADGSIVSFRADANAARLRSSARRLAIPELPDAVFIESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRPA
TQYRYLLIASPAGAYFKGGIAPVSVWVSTEYVRACPGGTGAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEEM
GGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERRIDIDEWQKKAAAGEITEVFACGTAAVITPV
ARVRHGASEFRIADGQPGEVTMALRDTLTGIQRGTFADTHGWMARLG

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI38176287, Length=346, Percent_Identity=38.150289017341, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI296010906, Length=346, Percent_Identity=38.150289017341, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI296010904, Length=346, Percent_Identity=38.150289017341, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI50658084, Length=338, Percent_Identity=39.0532544378698, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI296010902, Length=346, Percent_Identity=34.1040462427746, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI296010900, Length=346, Percent_Identity=34.1040462427746, Blast_Score=197, Evalue=9e-51,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=39.4557823129252, Blast_Score=197, Evalue=1e-50,
Organism=Escherichia coli, GI48994963, Length=330, Percent_Identity=31.8181818181818, Blast_Score=124, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=344, Percent_Identity=38.6627906976744, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17565728, Length=367, Percent_Identity=34.6049046321526, Blast_Score=188, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6322002, Length=345, Percent_Identity=38.8405797101449, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322608, Length=347, Percent_Identity=37.7521613832853, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24641779, Length=341, Percent_Identity=40.4692082111437, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ILVE_MYCTU (Q10399)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   C70786
- RefSeq:   NP_216726.1
- RefSeq:   NP_336738.1
- PDB:   3HT5
- PDBsum:   3HT5
- ProteinModelPortal:   Q10399
- SMR:   Q10399
- EnsemblBacteria:   EBMYCT00000003470
- EnsemblBacteria:   EBMYCT00000073103
- GeneID:   888352
- GeneID:   924159
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2266
- KEGG:   mtu:Rv2210c
- TIGR:   MT2266
- TubercuList:   Rv2210c
- GeneTree:   EBGT00050000017720
- HOGENOM:   HBG617123
- OMA:   YLRPFMF
- ProtClustDB:   PRK13357
- BRENDA:   2.6.1.42
- GO:   GO:0040007
- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786
- PANTHER:   PTHR11825
- PANTHER:   PTHR11825:SF2
- PIRSF:   PIRSF006468
- TIGRFAMs:   TIGR01123

Pfam domain/function: PF01063 Aminotran_4; SSF56752 Aminotrans_IV

EC number: =2.6.1.42

Molecular weight: Translated: 39666; Mature: 39535

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: BINDING 101-101 BINDING 209-209 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGSLQFTVLRAVNPATDAQRESMLREPGFGKYHTDHMVSIDYAEGRGWHNARVIPYGP
CCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCC
IELDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRADANAARLRSSARRLAIPELPDAVFI
CEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHH
ESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRPATQYRYLLIASPAGAYFKGG
HHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCHHCCC
IAPVSVWVSTEYVRACPGGTGAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEE
CCEEEEEEEHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
MGGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERRIDIDEWQKK
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHHCCCCHHHHHHH
AAAGEITEVFACGTAAVITPVARVRHGASEFRIADGQPGEVTMALRDTLTGIQRGTFADT
HHCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCCC
HGWMARLG
CCHHHHCC
>Mature Secondary Structure 
TSGSLQFTVLRAVNPATDAQRESMLREPGFGKYHTDHMVSIDYAEGRGWHNARVIPYGP
CCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCC
IELDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRADANAARLRSSARRLAIPELPDAVFI
CEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHH
ESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRPATQYRYLLIASPAGAYFKGG
HHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCCHHCCC
IAPVSVWVSTEYVRACPGGTGAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEE
CCEEEEEEEHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
MGGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERRIDIDEWQKK
HCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHHCCCCHHHHHHH
AAAGEITEVFACGTAAVITPVARVRHGASEFRIADGQPGEVTMALRDTLTGIQRGTFADT
HHCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCCC
HGWMARLG
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036