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The map label for this gene is thcD [H]

Identifier: 148823080

GI number: 148823080

Start: 2127651

End: 2128886

Strand: Reverse

Name: thcD [H]

Synonym: TBFG_11897

Alternate gene names: 148823080

Gene position: 2128886-2127651 (Counterclockwise)

Preceding gene: 148823081

Following gene: 148823076

Centisome position: 48.12

GC content: 64.56

Gene sequence:

>1236_bases
ATGGCCAGCTCAACCACGTTCGTCATTGTTGGCGGGGGACTTGCCGGGGCTAAAGCCGTAGAGGCGTTGCGCCGCAGCGA
CTTCGGCGGTCGGATTATCTTGTTCGGCGACGAAGAGCACCTGCCCTACGACCGGCCGCCGCTTTCCAAGGAGTTTCTGG
CCGGCAAAAAGTCGCTGAGCGACTTCACCATCCAGACCTCCGATTGGTACCGCGACCATGACGTGGATGTGCGGCTCGGC
GTGCGGGTGTCGTCATTGGATCGGTCCGCACACACGGTAGAGCTTCCCGACGGCGCTGCGGTGCGCTACGACAAGCTGCT
GTTGGCCACGGGATCGGCCCCACGGCGCCCACCAATACCTGGATCCGATGCCGCCGGCGTCCACTACCTGCGCAGCTACA
ACGACGCCGTGGCATTGAACTCCGTTCTGGTGCAGGGGTCTTCCCTCGCAGTGGTGGGTGCCGGCTGGATAGGCCTGGAA
GTGGCCGCCAGTGCGCGTCAACGTGGCGTCGACGTCACCGTCGTCGAGACCGCCATACAACCGCTGCTGGCCGCGCTCGG
GGAAGCGGTTGGCAAAGTGTTTGCCGACCTACATCGAGATCAAGGGGTGGACTTACGGTTGCAGACCCAGCTCGAAGAGA
TCACTGCAGCCGACGGCAAGGCGACCGGGCTCAAGATGCGTGACGGGTCGACGGTTGCCGCCGACGCCGTGCTAGTCGCT
GTGGGCGCGAAGCCCAACGTAGAACTCGCCCAACAGGCCGGGCTGGCGATGGGCGAGGGCGGTGTGTTGGTTGATGCATC
GCTGCGCACCAGCGATCCCGACATCTACGCAGTCGGCGACATTGCCGCCGCTGAACACCCGCTATTGGGTACCCGCGTCC
GTACCGAACACTGGGCCAACGCGCTCAAACAACCCGCGGTGGCGGCTGCCGGCATGCTGGGCCGGCCGGGCGAATACGCT
GAACTGCCCTATTTGTTCACCGATCAATACGACCTCGGCATGGAGTACGTCGGCCACGCCCCCAGCTGTGATCGGGTGGT
GTTCCGTGGCAACGTTGCCGGCCGCGAGTTCCTCTCCTTCTGGCTCGACGGCGACAGCCGGGTGCTAGCCGGGATGAATG
TCAACGTCTGGGACGTGGTGGACGACGTCAAGGGCCTGATCCGGTCCGGGAACCCGGTCGACGTTGACAGGCTGGTTGAT
CCGCAGTGGCCGTTGGCGGACCTGACGACGAATTGA

Upstream 100 bases:

>100_bases
AGACGACGAGCTGCGCCTGCAGGTGACCGGTTGACCCAGGTACGCGACCGGCTCGTAGGCTGTCAAGCGGCACCGCACAC
AACAGGACGGAGCAGCAATT

Downstream 100 bases:

>100_bases
CGTGTGTTGACCGCTACTCCACGGGTACCCAGGCATGATCGATAACGTCTGGCGGGCGCTGTGCCTGCAGGCGTTCTCGT
TCGGCGCGCCGACGTTTGAT

Product: reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MASSTTFVIVGGGLAGAKAVEALRRSDFGGRIILFGDEEHLPYDRPPLSKEFLAGKKSLSDFTIQTSDWYRDHDVDVRLG
VRVSSLDRSAHTVELPDGAAVRYDKLLLATGSAPRRPPIPGSDAAGVHYLRSYNDAVALNSVLVQGSSLAVVGAGWIGLE
VAASARQRGVDVTVVETAIQPLLAALGEAVGKVFADLHRDQGVDLRLQTQLEEITAADGKATGLKMRDGSTVAADAVLVA
VGAKPNVELAQQAGLAMGEGGVLVDASLRTSDPDIYAVGDIAAAEHPLLGTRVRTEHWANALKQPAVAAAGMLGRPGEYA
ELPYLFTDQYDLGMEYVGHAPSCDRVVFRGNVAGREFLSFWLDGDSRVLAGMNVNVWDVVDDVKGLIRSGNPVDVDRLVD
PQWPLADLTTN

Sequences:

>Translated_411_residues
MASSTTFVIVGGGLAGAKAVEALRRSDFGGRIILFGDEEHLPYDRPPLSKEFLAGKKSLSDFTIQTSDWYRDHDVDVRLG
VRVSSLDRSAHTVELPDGAAVRYDKLLLATGSAPRRPPIPGSDAAGVHYLRSYNDAVALNSVLVQGSSLAVVGAGWIGLE
VAASARQRGVDVTVVETAIQPLLAALGEAVGKVFADLHRDQGVDLRLQTQLEEITAADGKATGLKMRDGSTVAADAVLVA
VGAKPNVELAQQAGLAMGEGGVLVDASLRTSDPDIYAVGDIAAAEHPLLGTRVRTEHWANALKQPAVAAAGMLGRPGEYA
ELPYLFTDQYDLGMEYVGHAPSCDRVVFRGNVAGREFLSFWLDGDSRVLAGMNVNVWDVVDDVKGLIRSGNPVDVDRLVD
PQWPLADLTTN
>Mature_410_residues
ASSTTFVIVGGGLAGAKAVEALRRSDFGGRIILFGDEEHLPYDRPPLSKEFLAGKKSLSDFTIQTSDWYRDHDVDVRLGV
RVSSLDRSAHTVELPDGAAVRYDKLLLATGSAPRRPPIPGSDAAGVHYLRSYNDAVALNSVLVQGSSLAVVGAGWIGLEV
AASARQRGVDVTVVETAIQPLLAALGEAVGKVFADLHRDQGVDLRLQTQLEEITAADGKATGLKMRDGSTVAADAVLVAV
GAKPNVELAQQAGLAMGEGGVLVDASLRTSDPDIYAVGDIAAAEHPLLGTRVRTEHWANALKQPAVAAAGMLGRPGEYAE
LPYLFTDQYDLGMEYVGHAPSCDRVVFRGNVAGREFLSFWLDGDSRVLAGMNVNVWDVVDDVKGLIRSGNPVDVDRLVDP
QWPLADLTTN

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=398, Percent_Identity=32.1608040201005, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI65787454, Length=398, Percent_Identity=32.1608040201005, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI226437568, Length=398, Percent_Identity=32.1608040201005, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI4757732, Length=372, Percent_Identity=28.2258064516129, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI22202629, Length=372, Percent_Identity=28.2258064516129, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI195927006, Length=191, Percent_Identity=27.7486910994764, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788892, Length=406, Percent_Identity=34.9753694581281, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789765, Length=292, Percent_Identity=30.8219178082192, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789065, Length=282, Percent_Identity=30.8510638297872, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786307, Length=202, Percent_Identity=33.1683168316832, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=366, Percent_Identity=26.5027322404372, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564386, Length=332, Percent_Identity=29.5180722891566, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI281359715, Length=398, Percent_Identity=30.9045226130653, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI281359713, Length=398, Percent_Identity=30.9045226130653, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24639250, Length=398, Percent_Identity=30.9045226130653, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI18543267, Length=398, Percent_Identity=30.9045226130653, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24639252, Length=398, Percent_Identity=30.9045226130653, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24639257, Length=398, Percent_Identity=30.9045226130653, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24585130, Length=401, Percent_Identity=28.1795511221945, Blast_Score=178, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24581020, Length=384, Percent_Identity=26.3020833333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28573993, Length=382, Percent_Identity=26.9633507853403, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43630; Mature: 43498

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSTTFVIVGGGLAGAKAVEALRRSDFGGRIILFGDEEHLPYDRPPLSKEFLAGKKSLS
CCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCC
DFTIQTSDWYRDHDVDVRLGVRVSSLDRSAHTVELPDGAAVRYDKLLLATGSAPRRPPIP
CEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCC
GSDAAGVHYLRSYNDAVALNSVLVQGSSLAVVGAGWIGLEVAASARQRGVDVTVVETAIQ
CCCCCHHHHHHHCCCHHEEHHHEECCCCEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHH
PLLAALGEAVGKVFADLHRDQGVDLRLQTQLEEITAADGKATGLKMRDGSTVAADAVLVA
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEECCCCCEEECEEEEE
VGAKPNVELAQQAGLAMGEGGVLVDASLRTSDPDIYAVGDIAAAEHPLLGTRVRTEHWAN
ECCCCCHHHHHHCCCEECCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCEECHHHHHH
ALKQPAVAAAGMLGRPGEYAELPYLFTDQYDLGMEYVGHAPSCDRVVFRGNVAGREFLSF
HHHCCHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCCEEEEECCCCHHHHHEE
WLDGDSRVLAGMNVNVWDVVDDVKGLIRSGNPVDVDRLVDPQWPLADLTTN
EECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSTTFVIVGGGLAGAKAVEALRRSDFGGRIILFGDEEHLPYDRPPLSKEFLAGKKSLS
CCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCC
DFTIQTSDWYRDHDVDVRLGVRVSSLDRSAHTVELPDGAAVRYDKLLLATGSAPRRPPIP
CEEEECCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCC
GSDAAGVHYLRSYNDAVALNSVLVQGSSLAVVGAGWIGLEVAASARQRGVDVTVVETAIQ
CCCCCHHHHHHHCCCHHEEHHHEECCCCEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHH
PLLAALGEAVGKVFADLHRDQGVDLRLQTQLEEITAADGKATGLKMRDGSTVAADAVLVA
HHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEECCCCCEEECEEEEE
VGAKPNVELAQQAGLAMGEGGVLVDASLRTSDPDIYAVGDIAAAEHPLLGTRVRTEHWAN
ECCCCCHHHHHHCCCEECCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCEECHHHHHH
ALKQPAVAAAGMLGRPGEYAELPYLFTDQYDLGMEYVGHAPSCDRVVFRGNVAGREFLSF
HHHCCHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCCEEEEECCCCHHHHHEE
WLDGDSRVLAGMNVNVWDVVDDVKGLIRSGNPVDVDRLVDPQWPLADLTTN
EECCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]