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The map label for this gene is hcaD [C]

Identifier: 148823025

GI number: 148823025

Start: 2062409

End: 2063602

Strand: Reverse

Name: hcaD [C]

Synonym: TBFG_11842

Alternate gene names: 148823025

Gene position: 2063602-2062409 (Counterclockwise)

Preceding gene: 148823026

Following gene: 148823018

Centisome position: 46.64

GC content: 66.83

Gene sequence:

>1194_bases
GTGGTGGTGATCGGCTCCGGGTTCGCGGGCCTGTGGGCGGCGCTCGGTGCGGCCCGACGACTCGACGAGCTCGCCGTCCC
AGCGGGCACGGTCGACGTCATGGTGGTGAGCAACAAACCGTTTCATGATATCCGGGTTCGCAACTATGAGGCTGATCTGA
GCGCCTGCCGCATCCCGCTCGGCGACGTGCTCGGCCCTGCCGGCGTTGCGCATGTCACCGCCGAGGTGACCGCGATCGAC
GCCGACGGCCGCCGCGTCACCACGTCGACCGGTGCCTCATATAGCTATGACCGACTGGTCCTCGCGTCGGGCAGTCACGT
GGTCAAACCCGCGTTGCCAGGACTGGCAGAGTTCGGTTTTGATGTCGACACGTACGACGGCGCGGTCAGGCTACAGCAGC
ATCTGCAAGGACTGGCCGGCGGGCCGCTGACATCGGCGGCGGCAACCGTCGTGGTCGTCGGTGCCGGGCTGACCGGTATC
GAGACGGCCTGCGAGTTGCCGGGCCGACTGCACGCGTTGTTCGCCCGTGGTGATGGTGTCACCCCCCGCGTGGTACTCAT
CGACCACAACCCCTTCGTCGGCTCCGATATGGGTTTATCGGCGCGGCCGGTGATCGAACAGGCGCTGTTGGACAACGGCG
TCGAGACCAGAACCGGCGTGAGCGTTGCCGCCGTCAGCCCGGGCGGTGTAACGCTGTCATCGGGGGAACGGCTGGCAGCG
GCCACCGTGGTGTGGTGCGCCGGCATGCGGGCCAGCCGCCTGACCGAGCAGCTGCCGGTAGCCCGTGATCGGCTGGGACG
CCTGCAAGTCGACGACTACCTGCGGGTGATCGGGGTGCCTGCGATGTTTGCTGCTGGCGATGTGGCCGCGGCCCGGATGG
ATGACGAGCACCTGTCGGTCATGTCGTGTCAGCATGGGCGCCCGATGGGCCGCTATGCCGGCTGCAACGTCATCAACGAT
CTGTTCGACCAACCGTTGCTGGCTCTTCGGATCCCTTGGTATGTCACGGTTCTCGATCTAGGATCGGCGGGCGCGGTGTA
CACCGAGGGATGGGAACGCAAAGTGGTATCGCAGGGCGCACCGGCCAAGACCACCAAGCAGTCAATCAATACCCGGCGGA
TCTACCCGCCGCTGAACGGCAGTCGGGCAGACTTGCTGGCTGCGGCCGCGCCGCGAGTACAGCCCCGCCCCTAG

Upstream 100 bases:

>100_bases
CCGGGCTCGAAGGAGGTTGGCACGTGTGGATGTTCCTGCCCGGTCGCCACGCGGCGGCGGCGATACCCCAGAGTCGTCAG
GTGTTTAGCCGTTGACCCGG

Downstream 100 bases:

>100_bases
TGTCGTGGGTCATTCGGCCTGCGCGTGCTCACCGGCATACCAGTGCACAGCGTAAACGCCCGGCTGCAGCGACAGTTCCG
CCACCAACCGCTCTAGCTTG

Product: dehydrogenase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MVVIGSGFAGLWAALGAARRLDELAVPAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPLGDVLGPAGVAHVTAEVTAID
ADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGI
ETACELPGRLHALFARGDGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGERLAA
ATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSVMSCQHGRPMGRYAGCNVIND
LFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP

Sequences:

>Translated_397_residues
MVVIGSGFAGLWAALGAARRLDELAVPAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPLGDVLGPAGVAHVTAEVTAID
ADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGI
ETACELPGRLHALFARGDGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGERLAA
ATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSVMSCQHGRPMGRYAGCNVIND
LFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP
>Mature_397_residues
MVVIGSGFAGLWAALGAARRLDELAVPAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPLGDVLGPAGVAHVTAEVTAID
ADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGI
ETACELPGRLHALFARGDGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGERLAA
ATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSVMSCQHGRPMGRYAGCNVIND
LFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP

Specific function: Part Of Multicomponent 3-Phenylpropionate Dioxygenase. That Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG1252

COG function: function code C; NADH dehydrogenase, FAD-containing subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH dehydrogenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 41584; Mature: 41584

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVIGSGFAGLWAALGAARRLDELAVPAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPL
CEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECEECCHHHHCCCH
GDVLGPAGVAHVTAEVTAIDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGF
HHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCEEEEECCCEEECCCCCCHHHHCC
DVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGIETACELPGRLHALFARGDGV
CCCCCCHHHHHHHHHHHCCCCCHHHHCEEEEEEECCCCCHHHHHCCCCCEEEEEECCCCC
TPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGERLAA
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCHHH
ATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSV
HHHHHHCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCHHHHCCCHHHHHCCCHHHHE
MSCQHGRPMGRYAGCNVINDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGA
EECCCCCCCCHHHCHHHHHHHHCCCEEEEECCEEEEEEECCCCCCEECCCHHHHHHHCCC
PAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP
CCCHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVVIGSGFAGLWAALGAARRLDELAVPAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPL
CEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECEECCHHHHCCCH
GDVLGPAGVAHVTAEVTAIDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGF
HHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCEEEEECCCEEECCCCCCHHHHCC
DVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGIETACELPGRLHALFARGDGV
CCCCCCHHHHHHHHHHHCCCCCHHHHCEEEEEEECCCCCHHHHHCCCCCEEEEEECCCCC
TPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGERLAA
CCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCHHH
ATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSV
HHHHHHCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCHHHHCCCHHHHHCCCHHHHE
MSCQHGRPMGRYAGCNVINDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGA
EECCCCCCCCHHHCHHHHHHHHCCCEEEEECCEEEEEEECCCCCCEECCCHHHHHHHCCC
PAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP
CCCHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]