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The map label for this gene is mdh [H]

Identifier: 148822456

GI number: 148822456

Start: 1386979

End: 1387968

Strand: Direct

Name: mdh [H]

Synonym: TBFG_11265

Alternate gene names: 148822456

Gene position: 1386979-1387968 (Clockwise)

Preceding gene: 148822454

Following gene: 148822457

Centisome position: 31.35

GC content: 67.27

Gene sequence:

>990_bases
GTGAGCGCTAGTCCTCTCAAGGTCGCCGTTACCGGCGCCGCCGGCCAAATCGGCTACAGCCTGTTGTTCCGCCTGGCCAG
CGGCTCTTTGCTGGGCCCTGACCGTCCGATCGAGCTGCGGCTGCTCGAGATCGAGCCGGCACTGCAGGCGCTCGAGGGTG
TGGTGATGGAACTCGACGACTGCGCTTTCCCGCTGTTGTCCGGGGTGGAGATCGGTTCCGATCCCCAGAAGATCTTCGAT
GGTGTGAGCCTGGCCCTGCTGGTCGGAGCCCGCCCCCGGGGCGCGGGCATGGAGCGAAGTGACCTGCTGGAGGCCAACGG
CGCGATCTTCACCGCTCAGGGCAAAGCCCTCAACGCTGTCGCCGCGGATGACGTTCGCGTCGGGGTGACCGGCAACCCCG
CCAACACCAACGCGCTGATCGCGATGACCAATGCGCCCGACATTCCCCGCGAGCGGTTCTCGGCGCTCACCCGGCTGGAC
CACAATCGGGCGATCTCGCAGCTGGCCGCCAAGACCGGCGCGGCGGTCACCGACATCAAGAAGATGACGATCTGGGGCAA
TCACTCGGCCACCCAGTACCCCGACCTGTTCCACGCGGAGGTCGCCGGAAAGAACGCGGCCGAAGTGGTCAACGACCAGG
CCTGGATCGAGGATGAATTCATCCCGACGGTCGCCAAGCGCGGTGCGGCGATCATCGATGCGCGCGGCGCGTCGTCGGCC
GCCTCGGCCGCGTCGGCAACCATCGACGCTGCCCGGGACTGGTTGCTGGGGACGCCGGCGGACGATTGGGTCTCGATGGC
CGTCGTCTCCGACGGGTCCTACGGGGTGCCGGAGGGCTTGATCTCCTCGTTTCCGGTCACCACCAAGGGCGGCAACTGGA
CGATCGTGAGCGGCTTGGAGATCGACGAGTTCTCCGGCGGCCGGATCGACAAGTCAACCGCCGAGTTGGCTGACGAGCGC
AGCGCGGTCACCGAGCTCGGCCTGATCTGA

Upstream 100 bases:

>100_bases
AAACGTCAGTCGTAGCTGTGGGGGTTGGACCCCAGATGTCCGTCCGGTGCCGGTGCGCTAGTTTCAACCCGAAGCCAAGT
CCGTAAGGAGCAGAACCGAC

Downstream 100 bases:

>100_bases
GCGCAGGTCAGCCGCGCACTGAGCGGAGCCCGAGTCATCTTGACGTGTGTTTGTCCAGGCATCATGATGACCTGTATGCG
CACCACCTTGACGCTCGATG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSASPLKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEIEPALQALEGVVMELDDCAFPLLSGVEIGSDPQKIFD
GVSLALLVGARPRGAGMERSDLLEANGAIFTAQGKALNAVAADDVRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLD
HNRAISQLAAKTGAAVTDIKKMTIWGNHSATQYPDLFHAEVAGKNAAEVVNDQAWIEDEFIPTVAKRGAAIIDARGASSA
ASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSGGRIDKSTAELADER
SAVTELGLI

Sequences:

>Translated_329_residues
MSASPLKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEIEPALQALEGVVMELDDCAFPLLSGVEIGSDPQKIFD
GVSLALLVGARPRGAGMERSDLLEANGAIFTAQGKALNAVAADDVRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLD
HNRAISQLAAKTGAAVTDIKKMTIWGNHSATQYPDLFHAEVAGKNAAEVVNDQAWIEDEFIPTVAKRGAAIIDARGASSA
ASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSGGRIDKSTAELADER
SAVTELGLI
>Mature_328_residues
SASPLKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEIEPALQALEGVVMELDDCAFPLLSGVEIGSDPQKIFDG
VSLALLVGARPRGAGMERSDLLEANGAIFTAQGKALNAVAADDVRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLDH
NRAISQLAAKTGAAVTDIKKMTIWGNHSATQYPDLFHAEVAGKNAAEVVNDQAWIEDEFIPTVAKRGAAIIDARGASSAA
SAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLEIDEFSGGRIDKSTAELADERS
AVTELGLI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=330, Percent_Identity=51.5151515151515, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI89886456, Length=330, Percent_Identity=25.7575757575758, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=54.2944785276074, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=266, Percent_Identity=53.0075187969925, Blast_Score=250, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=54.7058823529412, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24583394, Length=322, Percent_Identity=52.7950310559006, Blast_Score=320, Evalue=9e-88,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34223; Mature: 34092

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASPLKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEIEPALQALEGVVMELDD
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHH
CAFPLLSGVEIGSDPQKIFDGVSLALLVGARPRGAGMERSDLLEANGAIFTAQGKALNAV
HHHHHHCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCEEEEE
AADDVRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLDHNRAISQLAAKTGAAVTDIK
ECCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEE
KMTIWGNHSATQYPDLFHAEVAGKNAAEVVNDQAWIEDEFIPTVAKRGAAIIDARGASSA
EEEEECCCCCCCCCCHHHHHHCCCCHHHHHCCCHHCCHHHHHHHHHCCCEEEECCCCCCH
ASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLE
HHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCCCHHHHHCCCEEECCCCEEEEECCE
IDEFSGGRIDKSTAELADERSAVTELGLI
EECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SASPLKVAVTGAAGQIGYSLLFRLASGSLLGPDRPIELRLLEIEPALQALEGVVMELDD
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHH
CAFPLLSGVEIGSDPQKIFDGVSLALLVGARPRGAGMERSDLLEANGAIFTAQGKALNAV
HHHHHHCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEECCCEEEEE
AADDVRVGVTGNPANTNALIAMTNAPDIPRERFSALTRLDHNRAISQLAAKTGAAVTDIK
ECCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEE
KMTIWGNHSATQYPDLFHAEVAGKNAAEVVNDQAWIEDEFIPTVAKRGAAIIDARGASSA
EEEEECCCCCCCCCCHHHHHHCCCCHHHHHCCCHHCCHHHHHHHHHCCCEEEECCCCCCH
ASAASATIDAARDWLLGTPADDWVSMAVVSDGSYGVPEGLISSFPVTTKGGNWTIVSGLE
HHHHHHHHHHHHHHCCCCCCHHHEEEEEEECCCCCCCHHHHHCCCEEECCCCEEEEECCE
IDEFSGGRIDKSTAELADERSAVTELGLI
EECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]