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The map label for this gene is metE [H]

Identifier: 148822347

GI number: 148822347

Start: 1262834

End: 1265218

Strand: Reverse

Name: metE [H]

Synonym: TBFG_11156

Alternate gene names: 148822347

Gene position: 1265218-1262834 (Counterclockwise)

Preceding gene: 148822348

Following gene: 148822343

Centisome position: 28.6

GC content: 69.06

Gene sequence:

>2385_bases
ATGGGCGTTTGGCCGTGCCTGTTGCCGGGTGCATCGGTGTGCACCTCAATCGGATCGGCTGCGCGTCCGCGGGCGGTGAA
CCACCCGTCGATCGAAGGACAGCACGTGACCCAGCCTGTACGTCGTCAACCCTTTACCGCAACCATCACCGGCTCCCCGC
GCATCGGCCCGCGCCGCGAACTCAAGCGCGCCACCGAAGGCTACTGGGCCGGACGTACCAGCCGATCCGAGCTGGAGGCC
GTCGCCGCCACGTTACGCCGCGACACCTGGTCGGCCCTGGCCGCGGCCGGTCTGGACTCGGTGCCGGTGAACACCTTCTC
CTACTACGACCAAATGCTCGATACCGCGGTGCTGCTCGGCGCGCTGCCGCCCCGAGTGAGCCCGGTTTCCGACGGGCTGG
ACCGCTATTTCGCCGCGGCGCGGGGCACCGACCAGATCGCGCCGCTGGAGATGACGAAGTGGTTCGACACCAACTACCAC
TACCTGGTACCCGAGATCGGGCCGTCGACCACGTTCACGCTGCACCCCGGCAAGGTGCTCGCCGAACTCAAAGAGGCGTT
AGGGCAAGGCATTCCCGCACGTCCGGTGATCATCGGGCCGATCACCTTCCTGCTGCTGAGCAAGGCCGTCGACGGCGCGG
GGGCGCCGATCGAACGCCTCGAAGAGTTGGTTCCGGTCTATTCGGAGCTGCTGTCGCTGCTTGCCGACGGCGGCGCCCAG
TGGGTGCAGTTCGACGAGCCGGCGCTGGTGACCGACCTCTCCCCCGACGCGCCCGCCCTGGCTGAAGCGGTGTACACCGC
GCTGTGCTCGGTGAGCAACCGGCCTGCGATCTATGTCGCCACCTACTTCGGGGACCCGGGCGCGGCCCTACCGGCGCTGG
CTCGCACCCCGGTCGAAGCCATCGGCGTCGACCTGGTGGCCGGTGCCGACACCTCGGTGGCCGGGGTACCCGAGCTGGCC
GGCAAGACGCTGGTGGCCGGGGTCGTCGACGGGCGCAACGTCTGGCGCACCGACCTGGAGGCGGCGTTGGGCACGTTGGC
GACCCTGCTGGGTTCGGCGGCTACCGTGGCCGTCTCGACGTCGTGCTCGACACTGCACGTGCCGTACTCGCTGGAACCGG
AAACCGACCTGGATGACGCGTTGCGGAGCTGGCTGGCGTTCGGTGCCGAAAAGGTGCGCGAAGTCGTCGTTCTCGCGCGT
GCCCTGCGCGACGGACACGACGCGGTCGCCGACGAGATCGCGTCGTCCCGCGCCGCCATCGCGTCCCGCAAGCGCGACCC
GCGGTTACACAATGGGCAAATCCGGGCGCGCATCGAGGCGATCGTCGCGTCCGGAGCCCACCGCGGCAATGCCGCCCAGC
GCCGCGCCAGCCAAGACGCGCGACTGCACCTGCCGCCGCTGCCGACCACGACGATCGGCTCCTACCCGCAGACCTCGGCG
ATCCGCGTTGCGCGTGCGGCGCTGCGGGCCGGTGAGATCGACGAGGCCGAGTACGTGCGCCGGATGCGGCAAGAGATCAC
CGAGGTGATCGCGCTACAGGAGCGGCTCGGGCTCGACGTGCTGGTGCACGGCGAACCGGAGCGCAACGACATGGTGCAGT
ACTTCGCCGAGCAATTGGCGGGTTTCTTCGCTACCCAGAACGGCTGGGTGCAGTCCTACGGCAGCCGCTGTGTGCGTCCG
CCGATCCTGTACGGCGACGTGTCCCGGCCGCGGGCGATGACGGTCGAGTGGATCACCTACGCGCAGTCGCTGACCGACAA
ACCGGTGAAGGGCATGTTGACCGGGCCGGTGACGATTCTGGCGTGGTCGTTCGTGCGTGACGACCAGCCGTTGGCCGATA
CCGCCAACCAGGTGGCGCTGGCGATTCGCGACGAGACCGTGGATTTGCAGTCCGCCGGCATCGCGGTCATCCAGGTCGAC
GAGCCTGCGCTGCGTGAACTGCTGCCGCTGCGTCGCGCCGACCAGGCCGAGTACTTGCGTTGGGCGGTAGGGGCTTTCCG
GTTGGCCACCTCCGGCGTCTCGGACGCCACCCAGATCCACACGCATCTGTGCTACTCGGAGTTCGGCGAGGTGATCGGCG
CGATCGCCGATCTGGACGCGGACGTCACGTCCATCGAGGCGGCCCGGTCACACATGGAGGTGCTCGACGACCTGAACGCG
ATCGGCTTCGCCAACGGTGTGGGCCCGGGCGTCTATGACATTCACTCGCCACGGGTGCCCTCCGCTGAGGAGATGGCCGA
CTCGTTGCGGGCCGCGTTGCGCGCGGTGCCGGCCGAGCGGCTGTGGGTCAACCCCGACTGCGGACTGAAGACCCGCAATG
TCGACGAGGTGACCGCGTCGCTGCACAACATGGTCGCCGCCGCCCGGGAGGTGCGCGCGGGCTAG

Upstream 100 bases:

>100_bases
CGACGGCCAGTAGGAGCACCCACCGGGTGCGAGCCTGCGAGTCCGAAGACCTGCCAGCCGTGCCGGACGCGCCGCGCCCG
GCGGCTCATCGCCTCGTGGA

Downstream 100 bases:

>100_bases
CTGACGAGCAGCTAGCCCGGCAGCACGTGGACCGGCACGTCGTCGTCCCAGGGCTGCCGGTTCACCATGTCGGCCACCCG
GACATAGCCGCGCTCGAACT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MGVWPCLLPGASVCTSIGSAARPRAVNHPSIEGQHVTQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEA
VAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDTAVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYH
YLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARPVIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQ
WVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRPAIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELA
GKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLAR
ALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSA
IRVARAALRAGEIDEAEYVRRMRQEITEVIALQERLGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRP
PILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVD
EPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNA
IGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG

Sequences:

>Translated_794_residues
MGVWPCLLPGASVCTSIGSAARPRAVNHPSIEGQHVTQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEA
VAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDTAVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYH
YLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARPVIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQ
WVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRPAIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELA
GKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLAR
ALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSA
IRVARAALRAGEIDEAEYVRRMRQEITEVIALQERLGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRP
PILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVD
EPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNA
IGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG
>Mature_793_residues
GVWPCLLPGASVCTSIGSAARPRAVNHPSIEGQHVTQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEAV
AATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDTAVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYHY
LVPEIGPSTTFTLHPGKVLAELKEALGQGIPARPVIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQW
VQFDEPALVTDLSPDAPALAEAVYTALCSVSNRPAIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELAG
KTLVAGVVDGRNVWRTDLEAALGTLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLARA
LRDGHDAVADEIASSRAAIASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSAI
RVARAALRAGEIDEAEYVRRMRQEITEVIALQERLGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRPP
ILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDE
PALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAI
GFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=48.074369189907, Blast_Score=684, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=768, Percent_Identity=45.9635416666667, Blast_Score=639, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85132; Mature: 85001

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVWPCLLPGASVCTSIGSAARPRAVNHPSIEGQHVTQPVRRQPFTATITGSPRIGPRRE
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHCCCCEEEECCCCCCCCHHH
LKRATEGYWAGRTSRSELEAVAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDTAVLLG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYHYLVPEIGPSTTFTLHPGKVL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCEEEECCCCCCCEEEECCHHHH
AELKEALGQGIPARPVIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQ
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
WVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRPAIYVATYFGDPGAALPALARTPVEA
EEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCHHH
IGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAATVAVST
HCCEEEECCCCCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
SCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLARALRDGHDAVADEIASSRAAI
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSA
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCHHH
IRVARAALRAGEIDEAEYVRRMRQEITEVIALQERLGLDVLVHGEPERNDMVQYFAEQLA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHH
GFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTIL
HHHHCCCCHHHHHCCCCCCCCEEECCCCCCCEEEHHHHHHHHHHCCCCCCCEECCCHHHH
AWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLR
HHHHHCCCCCHHHCHHHEEEEEECCCEEHHHCCEEEEEECCHHHHHHHHHHCCCHHHHHH
WAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTAS
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHHHH
LHNMVAAAREVRAG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
GVWPCLLPGASVCTSIGSAARPRAVNHPSIEGQHVTQPVRRQPFTATITGSPRIGPRRE
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHCCCCEEEECCCCCCCCHHH
LKRATEGYWAGRTSRSELEAVAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDTAVLLG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYHYLVPEIGPSTTFTLHPGKVL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCEEEECCCCCCCEEEECCHHHH
AELKEALGQGIPARPVIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQ
HHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
WVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRPAIYVATYFGDPGAALPALARTPVEA
EEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHCCHHH
IGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAATVAVST
HCCEEEECCCCCCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
SCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLARALRDGHDAVADEIASSRAAI
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSA
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCHHH
IRVARAALRAGEIDEAEYVRRMRQEITEVIALQERLGLDVLVHGEPERNDMVQYFAEQLA
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHH
GFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTIL
HHHHCCCCHHHHHCCCCCCCCEEECCCCCCCEEEHHHHHHHHHHCCCCCCCEECCCHHHH
AWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLR
HHHHHCCCCCHHHCHHHEEEEEECCCEEHHHCCEEEEEECCHHHHHHHHHHCCCHHHHHH
WAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAVPAERLWVNPDCGLKTRNVDEVTAS
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCHHHHHHH
LHNMVAAAREVRAG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]