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The map label for this gene is mesJ

Identifier: 148663488

GI number: 148663488

Start: 4072348

End: 4073319

Strand: Reverse

Name: mesJ

Synonym: MRA_3661

Alternate gene names: 148663488

Gene position: 4073319-4072348 (Counterclockwise)

Preceding gene: 148663489

Following gene: 148663487

Centisome position: 92.16

GC content: 70.88

Gene sequence:

>972_bases
ATGGATCGACAGAGTGCTGTAGCGCAGCTGCGTGCGGCTGCGGAGCAGTTCGCTCGGGTCCATCTCGACGCCTGCGACCG
TTGGAGCGTGGGGCTCTCCGGCGGCCCGGACTCGTTGGCGCTCACCGCTGTAGCCGCACGGCTGTGGCCCACCACCGCAC
TGATCGTGGACCACGGTCTGCAGCCCGGCTCGGCCACGGTCGCGGAAACAGCTCGCATACAAGCGATTTCGCTGGGATGT
GTGGACGCGCGGGTGCTTTGCGTTCAGGTGGGCGCGGCCGGCGGCCGGGAGGCGGCGGCGCGCAGCGCCCGCTACTCGGC
ATTGGAGGAGCACCGCGATGGCCCGGTGCTGTTGGCCCATACGCTCGATGATCAGGCCGAGACAGTGTTGTTGGGGCTTG
GCCGTGGTTCGGGGGCCCGGTCGATCGCCGGCATGCGTCCGTACGATCCGCCCTGGTGCCGGCCGCTGCTGGGGGTGCGC
CGCAGCGTGACACATGCGGCCTGCCGGGAGCTGGGCCTGACCGCCTGGCAGGATCCGCACAACACCGACCGCCGCTTCAC
CCGAACCCGATTGCGCACCGAAGTGCTGCCACTGCTGGAGGACGTGCTGGGCGGAGGTGTGGCCGAAGCGCTGGCTCGCA
CCGCGACAGCGTTGCGCGAGGACACTGACCTCATCGACACGATCGCTGCGCAGGCGCTGCCCGGAGCCGCTGTTGCTGGC
TCACGGGGACAGGAGTTGAGTACCAGCGCCTTGACCGCGCTGCCGGATGCGGTGCGGCGCCGGGTGATTCGGGGCTGGCT
GCTGGCCGGCGGCGCGACCGGACTGACCGACAGGCAGATCCGCGGAGTGGACCGGCTGGTCACCGCGTGGCGCGGTCAGG
GCGGGGTAGCGGTTGGGTCCACGTTGCGCGGTCAGCGACTGGTCGCCGGGCGGCGCGACGGCGTACTTGTGTTGCGGCGC
GAGCCCGTTTGA

Upstream 100 bases:

>100_bases
TCGACCATGTGGTCGACCGGGCGGGAATGAAGCTGTTCAACACGATCTGGTCGGGCCCCGAGACGCTGCCGTTGCCTGCC
GAGATCGAAAACCCGCAGCG

Downstream 100 bases:

>100_bases
CGCCCGCGTTGGTCGTTGGTCCCGCGGCGTGGCACGCTGTGCACGTGACCCAGAGCTCCTCGGCGATCACCCCCGGGCAG
ACGGCGGAGCTTTATCCGGG

Product: putative cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDRQSAVAQLRAAAEQFARVHLDACDRWSVGLSGGPDSLALTAVAARLWPTTALIVDHGLQPGSATVAETARIQAISLGC
VDARVLCVQVGAAGGREAAARSARYSALEEHRDGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPYDPPWCRPLLGVR
RSVTHAACRELGLTAWQDPHNTDRRFTRTRLRTEVLPLLEDVLGGGVAEALARTATALREDTDLIDTIAAQALPGAAVAG
SRGQELSTSALTALPDAVRRRVIRGWLLAGGATGLTDRQIRGVDRLVTAWRGQGGVAVGSTLRGQRLVAGRRDGVLVLRR
EPV

Sequences:

>Translated_323_residues
MDRQSAVAQLRAAAEQFARVHLDACDRWSVGLSGGPDSLALTAVAARLWPTTALIVDHGLQPGSATVAETARIQAISLGC
VDARVLCVQVGAAGGREAAARSARYSALEEHRDGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPYDPPWCRPLLGVR
RSVTHAACRELGLTAWQDPHNTDRRFTRTRLRTEVLPLLEDVLGGGVAEALARTATALREDTDLIDTIAAQALPGAAVAG
SRGQELSTSALTALPDAVRRRVIRGWLLAGGATGLTDRQIRGVDRLVTAWRGQGGVAVGSTLRGQRLVAGRRDGVLVLRR
EPV
>Mature_323_residues
MDRQSAVAQLRAAAEQFARVHLDACDRWSVGLSGGPDSLALTAVAARLWPTTALIVDHGLQPGSATVAETARIQAISLGC
VDARVLCVQVGAAGGREAAARSARYSALEEHRDGPVLLAHTLDDQAETVLLGLGRGSGARSIAGMRPYDPPWCRPLLGVR
RSVTHAACRELGLTAWQDPHNTDRRFTRTRLRTEVLPLLEDVLGGGVAEALARTATALREDTDLIDTIAAQALPGAAVAG
SRGQELSTSALTALPDAVRRRVIRGWLLAGGATGLTDRQIRGVDRLVTAWRGQGGVAVGSTLRGQRLVAGRRDGVLVLRR
EPV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=277, Percent_Identity=33.9350180505415, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_MYCBO (P67152)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857288.1
- ProteinModelPortal:   P67152
- SMR:   P67152
- EnsemblBacteria:   EBMYCT00000018041
- GeneID:   1093575
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3649c
- GeneTree:   EBGT00050000016642
- HOGENOM:   HBG692699
- OMA:   QDPHNSD
- ProtClustDB:   CLSK792603
- BioCyc:   MBOV233413:MB3649C-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946

EC number: NA

Molecular weight: Translated: 34130; Mature: 34130

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQSAVAQLRAAAEQFARVHLDACDRWSVGLSGGPDSLALTAVAARLWPTTALIVDHGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCC
QPGSATVAETARIQAISLGCVDARVLCVQVGAAGGREAAARSARYSALEEHRDGPVLLAH
CCCCCHHHHHHHEEEEEECHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
TLDDQAETVLLGLGRGSGARSIAGMRPYDPPWCRPLLGVRRSVTHAACRELGLTAWQDPH
ECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NTDRRFTRTRLRTEVLPLLEDVLGGGVAEALARTATALREDTDLIDTIAAQALPGAAVAG
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC
SRGQELSTSALTALPDAVRRRVIRGWLLAGGATGLTDRQIRGVDRLVTAWRGQGGVAVGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
TLRGQRLVAGRRDGVLVLRREPV
CCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MDRQSAVAQLRAAAEQFARVHLDACDRWSVGLSGGPDSLALTAVAARLWPTTALIVDHGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEECCCC
QPGSATVAETARIQAISLGCVDARVLCVQVGAAGGREAAARSARYSALEEHRDGPVLLAH
CCCCCHHHHHHHEEEEEECHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
TLDDQAETVLLGLGRGSGARSIAGMRPYDPPWCRPLLGVRRSVTHAACRELGLTAWQDPH
ECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NTDRRFTRTRLRTEVLPLLEDVLGGGVAEALARTATALREDTDLIDTIAAQALPGAAVAG
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC
SRGQELSTSALTALPDAVRRRVIRGWLLAGGATGLTDRQIRGVDRLVTAWRGQGGVAVGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECC
TLRGQRLVAGRRDGVLVLRREPV
CCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972