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The map label for this gene is 148663344

Identifier: 148663344

GI number: 148663344

Start: 3907586

End: 3909079

Strand: Reverse

Name: 148663344

Synonym: MRA_3520

Alternate gene names: NA

Gene position: 3909079-3907586 (Counterclockwise)

Preceding gene: 148663345

Following gene: 148663340

Centisome position: 88.44

GC content: 64.39

Gene sequence:

>1494_bases
GTGAGCCAGACGGCCCGGCGGTTGGGTCCGCAGGACATGTTTTTCCTGTACTCCGAATCGTCGACCACGATGATGCACGT
TGGTGCGTTGATGCCGTTCACGCCGCCCTCGGGTGCGCCGCCGGACTTGCTGCGCCAACTCGTCGACGAAAGCAAGGCCA
GCGAGGTCGTCGAACCCTGGAGCCTAAGACTGAGCCATCCGGAGCTGCTATACCATCCCACGCAGTCCTGGGTCGTTGAT
GACAACTTCGACCTGGACTACCACGTGCGCCGGTCGGCGCTGGCCAGCCCTGGCGACGAGCGTGAACTCGGGATCCCGGT
GTCCCGCTTGCATAGTCACGCCCTGGACCTACGCCGTCCGCCGTGGGAAGTGCATTTCATCGAAGGCCTCGAAGGTGGCC
GGTTCGCGATCTACATCAAGATGCACCACTCGTTGATAGACGGCTATACCGGCCAGAAAATGCTGGCCCGCAGCCTGTCC
ACCGACCCGCACGACACCACCCACCCGTTGTTCTTCAACATTCCCACACCTGGCCGATCACCCGCCGACACTCAAGACTC
CGTCGGCGGCGGTTTGATAGCTGGGGCCGGTAACGTGCTCGATGGCCTGGGCGACGTGGTGCGGGGGTTGGGCGGGCTGG
TCAGCGGGGTCGGTAGCGTCTTAGGTTCGGTGGCCGGTGCGGGACGATCGACGTTCGAGCTCACCAAGGCGCTGGTCAAC
GCCCAGCTGCGCAGTGATCACGAGTACCGCAACCTCGTCGGCTCGGTGCAAGCGCCGCACTGCATACTCAACACCCGCAT
CAGCCGCAACCGCCGCTTCGCCACCCAGCAATATCCGCTGGATCGACTGAAAGCCATTGGGGCTCAATATGATGCCACAA
TCAACGACGTCGCGTTGGCGATCATCGGAGGCGGCTTACGACGTTTTCTGGACGAGCTGGGTGAGCTGCCCAACAAGTCG
CTGATCGTGGTCTTGCCGGTCAACGTGCGACCCAAGGACGACGAGGGCGGCGGCAACGCGGTCGCGACCATCCTGGCGAC
GTTGGGTACCGACGTTGCCGATCCGGTGCAGCGGCTGGCGGCGGTCACGGCGTCAACCCGCGCGGCCAAGGCTCAGCTGA
GGTCCATGGACAAGGACGCGATCCTGGCCTATAGCGCCGCGTTGATGGCGCCGTACGGGGTGCAGTTGGCCAGCACGCTC
AGTGGGGTGAAGCCACCCTGGCCGTACACGTTCAACCTGTGTGTCAGCAATGTGCCGGGGCCCGAGGACGTGCTCTACCT
GCGGGGCAGCCGGATGGAGGCGTCCTATCCGGTCTCCCTGGTGGCGCACAGCCAGGCTCTCAACGTTACTTTGCAGAGTT
ACGCCGGCACGCTGAACTTTGGATTCATCGGCTGCCGTGACACACTGCCACACCTGCAGCGGCTCGCCGTCTACACCGGC
GAAGCGCTCGATCAGTTGGCGGCGGCTGACGGCGCCGCGGGCCTCGGTTCCTGA

Upstream 100 bases:

>100_bases
CGCGATGTGATGCGGCGTACAACGCACGGCCGCGATCATCTACGGTAGTTATCTGGCGGCGCTAGACGGCCTGAGAATGC
CTCCAACGAAGGAGTGGCCC

Downstream 100 bases:

>100_bases
ATTCCGGATCACCGCACGCATCGTCACCGTCGTCCTGGGGTGGCCGCTGCGATCATGATGGCCACCAGTGCGATGAGCCC
GACGACGACCAGGGGGTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3480c/MT3584

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPWSLRLSHPELLYHPTQSWVVD
DNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRPPWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLS
TDPHDTTHPLFFNIPTPGRSPADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVN
AQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALAIIGGGLRRFLDELGELPNKS
LIVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTL
SGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTG
EALDQLAAADGAAGLGS

Sequences:

>Translated_497_residues
MSQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPWSLRLSHPELLYHPTQSWVVD
DNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRPPWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLS
TDPHDTTHPLFFNIPTPGRSPADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVN
AQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALAIIGGGLRRFLDELGELPNKS
LIVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTL
SGVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTG
EALDQLAAADGAAGLGS
>Mature_496_residues
SQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPWSLRLSHPELLYHPTQSWVVDD
NFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRPPWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLST
DPHDTTHPLFFNIPTPGRSPADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVNA
QLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALAIIGGGLRRFLDELGELPNKSL
IVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLAAVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLS
GVKPPWPYTFNLCVSNVPGPEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTGE
ALDQLAAADGAAGLGS

Specific function: Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y3480_MYCTU (O06343)

Other databases:

- EMBL:   BX842583
- EMBL:   AE000516
- PIR:   E70568
- RefSeq:   NP_217997.1
- RefSeq:   NP_338129.1
- ProteinModelPortal:   O06343
- EnsemblBacteria:   EBMYCT00000002878
- EnsemblBacteria:   EBMYCT00000070087
- GeneID:   888473
- GeneID:   922922
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3584
- KEGG:   mtu:Rv3480c
- TIGR:   MT3584
- TubercuList:   Rv3480c
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   NARISRN
- ProtClustDB:   CLSK792514
- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 53315; Mature: 53184

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPW
CCCHHHHCCCCCEEEEEECCCCEEEEECEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
SLRLSHPELLYHPTQSWVVDDNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRP
HEECCCCCEEEECCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
PWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLSTDPHDTTHPLFFNIPTPGRS
CCEEEEEECCCCCEEEEEEEEEHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCC
PADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVN
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALA
HHHHCCHHHHHHHCCCCCCHHHHHCCHHCCCCCCHHCCCHHHHHHHCCCCCCCHHHHHHH
IIGGGLRRFLDELGELPNKSLIVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLA
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
AVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCC
PEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTG
CCCEEEEECCCCCCCCCEEEEECCHHHEEEHHHHCCCCCCCEEECHHHHHHHHHHHHHHH
EALDQLAAADGAAGLGS
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQTARRLGPQDMFFLYSESSTTMMHVGALMPFTPPSGAPPDLLRQLVDESKASEVVEPW
CCHHHHCCCCCEEEEEECCCCEEEEECEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
SLRLSHPELLYHPTQSWVVDDNFDLDYHVRRSALASPGDERELGIPVSRLHSHALDLRRP
HEECCCCCEEEECCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
PWEVHFIEGLEGGRFAIYIKMHHSLIDGYTGQKMLARSLSTDPHDTTHPLFFNIPTPGRS
CCEEEEEECCCCCEEEEEEEEEHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEECCCCCCC
PADTQDSVGGGLIAGAGNVLDGLGDVVRGLGGLVSGVGSVLGSVAGAGRSTFELTKALVN
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AQLRSDHEYRNLVGSVQAPHCILNTRISRNRRFATQQYPLDRLKAIGAQYDATINDVALA
HHHHCCHHHHHHHCCCCCCHHHHHCCHHCCCCCCHHCCCHHHHHHHCCCCCCCHHHHHHH
IIGGGLRRFLDELGELPNKSLIVVLPVNVRPKDDEGGGNAVATILATLGTDVADPVQRLA
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
AVTASTRAAKAQLRSMDKDAILAYSAALMAPYGVQLASTLSGVKPPWPYTFNLCVSNVPG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCC
PEDVLYLRGSRMEASYPVSLVAHSQALNVTLQSYAGTLNFGFIGCRDTLPHLQRLAVYTG
CCCEEEEECCCCCCCCCEEEEECCHHHEEEHHHHCCCCCCCEEECHHHHHHHHHHHHHHH
EALDQLAAADGAAGLGS
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036