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The map label for this gene is kgtP [H]

Identifier: 148663340

GI number: 148663340

Start: 3901048

End: 3902397

Strand: Reverse

Name: kgtP [H]

Synonym: MRA_3516

Alternate gene names: 148663340

Gene position: 3902397-3901048 (Counterclockwise)

Preceding gene: 148663344

Following gene: 148663337

Centisome position: 88.29

GC content: 59.93

Gene sequence:

>1350_bases
ATGACCGTTTCCATTGCACCTCCATCGCGCCCGAGTCAGGCCGAGACCCGTCGGGCCATTTGGAACACGATCCGGGGATC
GTCGGGCAATCTGGTCGAGTGGTATGACGTCTACGTCTACACCGTGTTCGCTACGTATTTCGAGGACCAATTCTTTGATC
GGGCCGATCGCAACTCGACGGTCTATGTCTACGCGATCTTCGCTGTTACCTTCGTGACCCGGCCCGTCGGATCGTGGTTC
CTTGGTCGGTTTGCCGACCGCCGCGGCCGCCGGGCCGCGCTGACGTTCAGTGTGTCGCTGATGGCCGCCTGTTCGCTGAT
CGTGGCGTTGGTGCCGTCTCGCTCGAGCATTGGCGTCGCTGCGCCGATCCTGCTTATTTTGTGTCGGCTCGTCCAGGGAT
TTGCGACCGGCGGAGAGTACGGCACGTCGGCGACGTACATGTCTGAGGCCGCGACGCGGGAGCGGCGCGGCTACTTCTCG
TCGTTTCAGTATGTGACGCTGGTGGGTGGGCACGTGCTTGCCCAGTTCACCCTGCTAGTCATCCTGGCGGTCTTCACCCG
CGAGCAGGTTCACGAATTCGGCTGGCGCATCGGCTTCGCCGTAGGCGGTGGCGCCGCGATCGTGGTGTTCTGGTTGCGGC
GCACGATGGATGAATCGCTCAGTCAGGAACGCCTGACTGCGATCAAGGCCGGGCGGGACCACGACTCCGGGTCGCTCCGT
GAACTGGCCACCCACTATTGGAAGCCATTGCTGCTCTGTTTCCTCGTCACGTTGGGCGGCACGGTCGCGTTTTATACCTA
CAGCGTCAATGCACCTGCGATCGTCAAGAGTGTCTACGGTTCGCAGGCGATGACCGCTACTTGGATCAACCTGGTTGGTC
TGATTCTGTTGATGATGCTGCAGCCCATCGGGGGCATGATCAGCGACAAGATCGGCCGCAAGCCACTGCTGCTGTGGTTC
GGCGTCGGCGGACTCATCTATACCTACGTCCTTGTTACTTATCTTCCCGAAACACGTTCGCCGACAATGTCGTTTCTATT
GGTGGCCGTTGGCTATGTGATTCTGACCGGATACTGCTCGATCAATGCACTGGTCAAGTCCGAACTGTTCCCCGCGCACG
TGCGCGCCCTTGGTGTCGGAGTCGGCTATGCACTGGCGAACTCGGTCTTCGGCGGCACCGCGCCGCTGATCTACCAGGCT
CTTAAGGAACGCGATCAGGTGCCGATGTTCATCGCCTACGTGACCGCGTGCATAGCGGTCTCGCTGATTGTCTATGTGTT
TTTCATCAAGAACAAGGCCGATACCTACCTCGACCGCGAGCAGGGATTTGCATTCTACGGGCACGCATAG

Upstream 100 bases:

>100_bases
TCAAAACCGACCACGCTCCAGCCCTGCACGGCGGGTGCTGACGTCGGTCACTATGGTGACCGTCAGACGGCGCGACAATG
CGAATCGCATGGAGGACACC

Downstream 100 bases:

>100_bases
CTGTAGCGTCTGGGTAACACCTTTCGCCGAGCGTGCAATCACTGCGAAAATGGCGCCTTGGGTTCCGTGGATGGACCACG
AACCGGGTGCCGGATTGACG

Product: dicarboxylic acid transport integral membrane protein KgtP

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTVSIAPPSRPSQAETRRAIWNTIRGSSGNLVEWYDVYVYTVFATYFEDQFFDRADRNSTVYVYAIFAVTFVTRPVGSWF
LGRFADRRGRRAALTFSVSLMAACSLIVALVPSRSSIGVAAPILLILCRLVQGFATGGEYGTSATYMSEAATRERRGYFS
SFQYVTLVGGHVLAQFTLLVILAVFTREQVHEFGWRIGFAVGGGAAIVVFWLRRTMDESLSQERLTAIKAGRDHDSGSLR
ELATHYWKPLLLCFLVTLGGTVAFYTYSVNAPAIVKSVYGSQAMTATWINLVGLILLMMLQPIGGMISDKIGRKPLLLWF
GVGGLIYTYVLVTYLPETRSPTMSFLLVAVGYVILTGYCSINALVKSELFPAHVRALGVGVGYALANSVFGGTAPLIYQA
LKERDQVPMFIAYVTACIAVSLIVYVFFIKNKADTYLDREQGFAFYGHA

Sequences:

>Translated_449_residues
MTVSIAPPSRPSQAETRRAIWNTIRGSSGNLVEWYDVYVYTVFATYFEDQFFDRADRNSTVYVYAIFAVTFVTRPVGSWF
LGRFADRRGRRAALTFSVSLMAACSLIVALVPSRSSIGVAAPILLILCRLVQGFATGGEYGTSATYMSEAATRERRGYFS
SFQYVTLVGGHVLAQFTLLVILAVFTREQVHEFGWRIGFAVGGGAAIVVFWLRRTMDESLSQERLTAIKAGRDHDSGSLR
ELATHYWKPLLLCFLVTLGGTVAFYTYSVNAPAIVKSVYGSQAMTATWINLVGLILLMMLQPIGGMISDKIGRKPLLLWF
GVGGLIYTYVLVTYLPETRSPTMSFLLVAVGYVILTGYCSINALVKSELFPAHVRALGVGVGYALANSVFGGTAPLIYQA
LKERDQVPMFIAYVTACIAVSLIVYVFFIKNKADTYLDREQGFAFYGHA
>Mature_448_residues
TVSIAPPSRPSQAETRRAIWNTIRGSSGNLVEWYDVYVYTVFATYFEDQFFDRADRNSTVYVYAIFAVTFVTRPVGSWFL
GRFADRRGRRAALTFSVSLMAACSLIVALVPSRSSIGVAAPILLILCRLVQGFATGGEYGTSATYMSEAATRERRGYFSS
FQYVTLVGGHVLAQFTLLVILAVFTREQVHEFGWRIGFAVGGGAAIVVFWLRRTMDESLSQERLTAIKAGRDHDSGSLRE
LATHYWKPLLLCFLVTLGGTVAFYTYSVNAPAIVKSVYGSQAMTATWINLVGLILLMMLQPIGGMISDKIGRKPLLLWFG
VGGLIYTYVLVTYLPETRSPTMSFLLVAVGYVILTGYCSINALVKSELFPAHVRALGVGVGYALANSVFGGTAPLIYQAL
KERDQVPMFIAYVTACIAVSLIVYVFFIKNKADTYLDREQGFAFYGHA

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=40.0473933649289, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=27.2093023255814, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788292, Length=412, Percent_Identity=27.9126213592233, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789941, Length=406, Percent_Identity=27.0935960591133, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=29.2792792792793, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082404, Length=341, Percent_Identity=24.3401759530792, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49517; Mature: 49386

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSIAPPSRPSQAETRRAIWNTIRGSSGNLVEWYDVYVYTVFATYFEDQFFDRADRNST
CEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYVYAIFAVTFVTRPVGSWFLGRFADRRGRRAALTFSVSLMAACSLIVALVPSRSSIGVA
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHH
APILLILCRLVQGFATGGEYGTSATYMSEAATRERRGYFSSFQYVTLVGGHVLAQFTLLV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHH
ILAVFTREQVHEFGWRIGFAVGGGAAIVVFWLRRTMDESLSQERLTAIKAGRDHDSGSLR
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ELATHYWKPLLLCFLVTLGGTVAFYTYSVNAPAIVKSVYGSQAMTATWINLVGLILLMML
HHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QPIGGMISDKIGRKPLLLWFGVGGLIYTYVLVTYLPETRSPTMSFLLVAVGYVILTGYCS
HHHCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
INALVKSELFPAHVRALGVGVGYALANSVFGGTAPLIYQALKERDQVPMFIAYVTACIAV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SLIVYVFFIKNKADTYLDREQGFAFYGHA
HHHHHHHHHCCCHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TVSIAPPSRPSQAETRRAIWNTIRGSSGNLVEWYDVYVYTVFATYFEDQFFDRADRNST
EEECCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYVYAIFAVTFVTRPVGSWFLGRFADRRGRRAALTFSVSLMAACSLIVALVPSRSSIGVA
EEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCHH
APILLILCRLVQGFATGGEYGTSATYMSEAATRERRGYFSSFQYVTLVGGHVLAQFTLLV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHH
ILAVFTREQVHEFGWRIGFAVGGGAAIVVFWLRRTMDESLSQERLTAIKAGRDHDSGSLR
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ELATHYWKPLLLCFLVTLGGTVAFYTYSVNAPAIVKSVYGSQAMTATWINLVGLILLMML
HHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
QPIGGMISDKIGRKPLLLWFGVGGLIYTYVLVTYLPETRSPTMSFLLVAVGYVILTGYCS
HHHCCHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
INALVKSELFPAHVRALGVGVGYALANSVFGGTAPLIYQALKERDQVPMFIAYVTACIAV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SLIVYVFFIKNKADTYLDREQGFAFYGHA
HHHHHHHHHCCCHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]