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The map label for this gene is nuoM

Identifier: 148663012

GI number: 148663012

Start: 3536288

End: 3537949

Strand: Direct

Name: nuoM

Synonym: MRA_3190

Alternate gene names: 148663012

Gene position: 3536288-3537949 (Clockwise)

Preceding gene: 148663011

Following gene: 148663013

Centisome position: 80.01

GC content: 64.32

Gene sequence:

>1662_bases
GTGAATAACGTGCCGTGGCTGAGCGTGCTCTGGCTGGTGCCGCTGGCAGGTGCGGTGCTGATCATCCTGCTACCACCCGG
TCGGCGCCGACTCGCCAAGTGGGCCGGTATGGTTGTCAGCGTCCTGACGTTGGCGGTGTCGATCGTCGTCGCGGCCGAAT
TCAAGCCCAGCGCCGAGCCGTATCAGTTCGTCGAAAAGCATTCCTGGATACCGGCGTTCGGCGCCGGCTATACCCTTGGT
GTGGACGGCATCGCAGTGGTGCTGGTGTTGTTGACCACAGTGCTGATTCCGTTGCTGCTGGTGGCCGGCTGGAACGACGC
AACCGATGCTGACGACCTGTCCCCCGCAAGCGGGAGGTACCCCCAGCGCCCGGCTCCGCCGCGCTTGCGATCGTCAGGTG
GCGAACGCACCCGAGGCGTGCACGCCTACGTGGCATTGACGCTGGCCATCGAGTCGATGGTGCTGATGTCGGTGATCGCG
CTGGACGTGCTGCTGTTCTACGTGTTCTTCGAGGCCATGCTGATCCCGATGTACTTCCTCATCGGCGGCTTCGGCCAGGG
GGCCGGACGCTCGCGTGCCGCGGTGAAGTTCTTGCTGTACAACCTGTTTGGCGGGTTGATCATGCTGGCGGCGGTGATCG
GGCTGTATGTGGTGACCGCACAGTACGATTCGGGCACCTTCGACTTCCGTGAGATCGTGGCCGGCGTGGCGGCGGGCCGC
TACGGAGCGGACCCGGCGGTGTTCAAGGCGCTGTTCTTGGGCTTCATGTTCGCGTTCGCGATCAAGGCTCCGCTGTGGCC
GTTCCATCGCTGGCTGCCGGACGCCGCCGTCGAGTCCACCCCAGCGACCGCGGTGCTGATGATGGCGGTGATGGACAAGG
TCGGCACCTTCGGCATGCTGCGCTACTGCCTGCAGCTGTTTCCTGACCCGTCAACGTATTTCCGTCCGCTGATCGTGACG
CTGGCCATCATCGGGGTGATCTACGGCGCGATCGTGGCGATCGGCCAAACCGACATGATGCGGCTGATCGCCTACACCTC
GATCTCGCACTTCGGGTTCATCATCGCAGGCATCTTCGTCATGACCACCCAGGGCCAGAGCGGGTCGACGCTGTACATGC
TCAACCACGGCCTGTCCACGGCGGCGGTGTTCCTGATCGCCGGTTTCTTGATAGCGCGGCGCGGCAGCCGATCGATCGCC
GACTACGGCGGTGTCCAGAAGGTGGCGCCCATCCTGGCCGGCACGTTCATGGTCTCGGCCATGGCCACCGTATCGCTGCC
CGGCCTAGCCCCGTTTATCAGCGAATTCCTGGTTCTGCTGGGCACTTTCAGCCGCTACTGGCTGGCGGCGGCGTTCGGCG
TTACCGCACTGGTCCTCTCGGCCGTTTACATGCTGTGGCTCTACCAGCGGGTGATGACCGGTCCGGTAGCCGAAGGCAAC
GAACGCATAGGGGATCTGGTGGGCCGCGAGATGATCGTGGTGGCACCGTTGATCGCGCTGTTACTCGTGCTTGGGGTCTA
CCCCAAACCTGTGCTCGACATCATCAATCCGGCGGTCGAGAACACCATGACCACCATCGGCCAGCATGATCCCGCGCCCA
GCGTGGCACACCCGGTTCCGGCCGTGGGCGCCTCCCGGACAGCCGAAGGACCGCACCCATGA

Upstream 100 bases:

>100_bases
GCCTGCGGCAAATGCAAACCGGCTTCGCCCGTAACTACGCGTTATCGATGCTGGTAGGAGCGGTGTTAGTGGCGGCGGCG
CTGCTGGTGGTGCAGCTGTG

Downstream 100 bases:

>100_bases
TCCTGCCCGCCCCGCACGTCGAGTACTTCCTGCTCGCTCCGATGCTCATCGTCTTTTCGGTTGCGGTCGCCGGTGTGCTG
GCCGAGGCTTTCCTGCCGCG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEPYQFVEKHSWIPAFGAGYTLG
VDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRYPQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIA
LDVLLFYVFFEAMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
YGADPAVFKALFLGFMFAFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGMLRYCLQLFPDPSTYFRPLIVT
LAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFVMTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIA
DYGGVQKVAPILAGTFMVSAMATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVPAVGASRTAEGPHP

Sequences:

>Translated_553_residues
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEPYQFVEKHSWIPAFGAGYTLG
VDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRYPQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIA
LDVLLFYVFFEAMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
YGADPAVFKALFLGFMFAFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGMLRYCLQLFPDPSTYFRPLIVT
LAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFVMTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIA
DYGGVQKVAPILAGTFMVSAMATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVPAVGASRTAEGPHP
>Mature_553_residues
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEPYQFVEKHSWIPAFGAGYTLG
VDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRYPQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIA
LDVLLFYVFFEAMLIPMYFLIGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
YGADPAVFKALFLGFMFAFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGMLRYCLQLFPDPSTYFRPLIVT
LAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFVMTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIA
DYGGVQKVAPILAGTFMVSAMATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVPAVGASRTAEGPHP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family

Homologues:

Organism=Homo sapiens, GI251831116, Length=388, Percent_Identity=28.0927835051546, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI251831117, Length=351, Percent_Identity=21.6524216524217, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788613, Length=534, Percent_Identity=31.6479400749064, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=26.2820512820513, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788831, Length=410, Percent_Identity=24.8780487804878, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788829, Length=508, Percent_Identity=24.4094488188976, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI145693160, Length=368, Percent_Identity=26.6304347826087, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788614, Length=362, Percent_Identity=26.7955801104972, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367154, Length=293, Percent_Identity=26.962457337884, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOM_MYCTU (O53307)

Other databases:

- EMBL:   BX842582
- EMBL:   AE000516
- PIR:   C70946
- RefSeq:   NP_217673.1
- RefSeq:   NP_337770.1
- ProteinModelPortal:   O53307
- EnsemblBacteria:   EBMYCT00000003347
- EnsemblBacteria:   EBMYCT00000071731
- GeneID:   888765
- GeneID:   923349
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3245
- KEGG:   mtu:Rv3157
- TIGR:   MT3245
- TubercuList:   Rv3157
- GeneTree:   EBGT00050000015714
- HOGENOM:   HBG643593
- OMA:   QTDVMRL
- ProtClustDB:   PRK05846
- BRENDA:   1.6.99.5
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- PRINTS:   PR01437
- TIGRFAMs:   TIGR01972

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 59208; Mature: 59208

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00673 V8_SER

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20f7e52c)-; HASH(0x1e13f83c)-; HASH(0x218ffbd0)-; HASH(0x1e13f7a0)-; HASH(0x218bd1a4)-; HASH(0x1e13f848)-; HASH(0x218c0bb0)-; HASH(0x1e13fa7c)-; HASH(0x218c0a3c)-; HASH(0x21904b08)-; HASH(0x1e13faac)-; HASH(0x218c0bf8)-; HASH(0x21902590)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEP
CCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YQFVEKHSWIPAFGAGYTLGVDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRY
HHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIALDVLLFYVFFEAMLIPMYFL
CCCCCCCCHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCC
YGADPAVFKALFLGFMFAFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGML
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
RYCLQLFPDPSTYFRPLIVTLAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
MTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIADYGGVQKVAPILAGTFMVSA
HEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHH
MATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AVGASRTAEGPHP
CCCCCCCCCCCCH
>Mature Secondary Structure
MNNVPWLSVLWLVPLAGAVLIILLPPGRRRLAKWAGMVVSVLTLAVSIVVAAEFKPSAEP
CCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YQFVEKHSWIPAFGAGYTLGVDGIAVVLVLLTTVLIPLLLVAGWNDATDADDLSPASGRY
HHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PQRPAPPRLRSSGGERTRGVHAYVALTLAIESMVLMSVIALDVLLFYVFFEAMLIPMYFL
CCCCCCCCHHCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGGFGQGAGRSRAAVKFLLYNLFGGLIMLAAVIGLYVVTAQYDSGTFDFREIVAGVAAGR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCC
YGADPAVFKALFLGFMFAFAIKAPLWPFHRWLPDAAVESTPATAVLMMAVMDKVGTFGML
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
RYCLQLFPDPSTYFRPLIVTLAIIGVIYGAIVAIGQTDMMRLIAYTSISHFGFIIAGIFV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
MTTQGQSGSTLYMLNHGLSTAAVFLIAGFLIARRGSRSIADYGGVQKVAPILAGTFMVSA
HEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHH
MATVSLPGLAPFISEFLVLLGTFSRYWLAAAFGVTALVLSAVYMLWLYQRVMTGPVAEGN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
ERIGDLVGREMIVVAPLIALLLVLGVYPKPVLDIINPAVENTMTTIGQHDPAPSVAHPVP
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AVGASRTAEGPHP
CCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036