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The map label for this gene is 148662941

Identifier: 148662941

GI number: 148662941

Start: 3465081

End: 3466499

Strand: Direct

Name: 148662941

Synonym: MRA_3119

Alternate gene names: NA

Gene position: 3465081-3466499 (Clockwise)

Preceding gene: 148662940

Following gene: 148662942

Centisome position: 78.4

GC content: 70.05

Gene sequence:

>1419_bases
ATGCGCAGGCTCAACGGCGTTGACGCGCTGATGCTGTATCTCGACGGCGGCAGCGCCTACAACCACACCCTCAAGATCAG
CGTGCTCGACCCGTCGACCGACCCGGACGGCTGGTCGTGGCCGAAGGCGCGGCAGATGTTCGAGGAGCGCGCCCACCTGC
TTCCGGTCTTCCGGCTGCGGTACCTGCCCACACCGCTGGGCCTGCATCACCCGATCTGGGTCGAGGATCCCGAATTCGAC
CTCGACGCGCACGTGCGCCGGGTCGTCTGTCCCGCCCCGGGCGGGATGGCGGAATTCTGCGCGCTCGTCGAGCAGATCTA
CGCCCACCCGCTGGATCGCGACCGCCCGCTGTGGCAGACCTGGGTGGTCGAGGGCCTCGACGGCGGCCGCGTCGCCCTGG
TCACGCTGCTGCACCACGCCTACTCCGACGGCGTCGGCGTGCTGGACATGCTCGCCGCGTTCTACAACGACACGCCTGAC
GAGGCCCCCGTGGTTGCGCCCCCGTGGGAGCCGCCGCCGCTGCCGTCCACCCGGCAACGCCTCGGTTGGGCCCTGCGGGA
CCTGCCCTCCAGGCTCGGCAAGATCGCGCCGACCGTGCGGGCCGTTCGTGATCGGGTGCGCATCGAACGGGAGTTCGCCA
AAGACGGCGACCGGCGCGTCCCGCCCACGTTCGACCGCTCCGCACCGCCGGGCCCGTTTCAGCGCGGGCTGTCGCGCAGC
CGGCGGTTCTCCTGCGAATCGTTCCCGCTCGCCGAGGTTCGCGAGGTGAGCAAGACGCTGGGCGTCACCATCAACGACGT
CTTTTTGGCGTGTGTGGCCGGTGCCGTTCGTCGCTATCTGGAGCGTTGCGGCTCCCCTCCCACCGACGCGATGGTGGCCA
CGATGCCGCTCGCGGTCACCCCGGCGGCCGAGCGCGCCCACCCCGGCAACTACTCGTCGGTCGACTACGTCTGGCTACGC
GCCGACATCGCCGACCCGCTCGAGCGGCTACACGCGACCCACCTCGCCGCCGAGGCCACCAAGCAGCACTTCGCCCAGAC
CAAGGACGCCGACGTCGGCGCGGTGGTCGAGCTGCTGCCGGAACGCCTCATCTCGGGCCTGGCGCGTGCCAACGCGCGCA
CCAAGGGCCGCTTCGACACCTTCAAGAACGTGGTCGTGTCCAACGTGCCGGGGCCGCGTGAGCCGCGGTATCTCGGCCGC
TGGCGCGTCGACCAGTGGTTTTCCACCGGGCAGATCTCCCACGGCGCCACGCTCAACATGACCGTCTGGAGCTATTGCGA
CCAGTTCAACCTGTGCGTAATGGCCGACGCAGTCGCGGTTCGGAACACCTGGGAATTGCTCGGCGGCTTCCGCGCCTCGC
ACGAGGAGCTGCTCGCGGCGGCCCGTGCCCAAGCCACGCCCAAGGAGATGGCCACATGA

Upstream 100 bases:

>100_bases
GTGAACCAGGGCTACCAGGATCTGCGGGACGGCAAGAACATTCGGGGCGTGATCGTGCACTGACCAGCTTCCACCAACCA
CGAATCCAGAGAGGACGATG

Downstream 100 bases:

>100_bases
CCCGCATCAATCCGATCGATCTGTCCTTCCTGCTGCTGGAGCGGGCCAACCGGCCCAACCACATGGCCGCCTACACGATC
TTCGAAAAGCCGAAAGGACA

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3087/MT3172

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLRYLPTPLGLHHPIWVEDPEFD
LDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQTWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPD
EAPVVAPPWEPPPLPSTRQRLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSRS
RRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVTPAAERAHPGNYSSVDYVWLR
ADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLPERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGR
WRVDQWFSTGQISHGATLNMTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT

Sequences:

>Translated_472_residues
MRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLRYLPTPLGLHHPIWVEDPEFD
LDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQTWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPD
EAPVVAPPWEPPPLPSTRQRLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSRS
RRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVTPAAERAHPGNYSSVDYVWLR
ADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLPERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGR
WRVDQWFSTGQISHGATLNMTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT
>Mature_472_residues
MRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLRYLPTPLGLHHPIWVEDPEFD
LDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQTWVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPD
EAPVVAPPWEPPPLPSTRQRLGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSRS
RRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVTPAAERAHPGNYSSVDYVWLR
ADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLPERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGR
WRVDQWFSTGQISHGATLNMTVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y3087_MYCTU (O53304)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   C70853
- RefSeq:   NP_217603.1
- RefSeq:   NP_337694.1
- ProteinModelPortal:   O53304
- EnsemblBacteria:   EBMYCT00000001092
- EnsemblBacteria:   EBMYCT00000070223
- GeneID:   888653
- GeneID:   926718
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3172
- KEGG:   mtu:Rv3087
- TIGR:   MT3172
- TubercuList:   Rv3087
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   RNTWELV
- ProtClustDB:   CLSK792272
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 52598; Mature: 52598

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLR
CCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YLPTPLGLHHPIWVEDPEFDLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQT
HCCCCCCCCCCEECCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
WVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPDEAPVVAPPWEPPPLPSTRQR
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHH
LGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
RRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVT
HCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEC
PAAERAHPGNYSSVDYVWLRADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLP
CCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGRWRVDQWFSTGQISHGATLNM
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHCCCCCCCCCEEEE
TVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MRRLNGVDALMLYLDGGSAYNHTLKISVLDPSTDPDGWSWPKARQMFEERAHLLPVFRLR
CCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YLPTPLGLHHPIWVEDPEFDLDAHVRRVVCPAPGGMAEFCALVEQIYAHPLDRDRPLWQT
HCCCCCCCCCCEECCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
WVVEGLDGGRVALVTLLHHAYSDGVGVLDMLAAFYNDTPDEAPVVAPPWEPPPLPSTRQR
HHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHHHH
LGWALRDLPSRLGKIAPTVRAVRDRVRIEREFAKDGDRRVPPTFDRSAPPGPFQRGLSRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
RRFSCESFPLAEVREVSKTLGVTINDVFLACVAGAVRRYLERCGSPPTDAMVATMPLAVT
HCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEC
PAAERAHPGNYSSVDYVWLRADIADPLERLHATHLAAEATKQHFAQTKDADVGAVVELLP
CCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ERLISGLARANARTKGRFDTFKNVVVSNVPGPREPRYLGRWRVDQWFSTGQISHGATLNM
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHCCCCCCCCCEEEE
TVWSYCDQFNLCVMADAVAVRNTWELLGGFRASHEELLAAARAQATPKEMAT
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036