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The map label for this gene is nrdE

Identifier: 148662904

GI number: 148662904

Start: 3424255

End: 3426336

Strand: Reverse

Name: nrdE

Synonym: MRA_3083

Alternate gene names: 148662904

Gene position: 3426336-3424255 (Counterclockwise)

Preceding gene: 148662905

Following gene: 148662903

Centisome position: 77.52

GC content: 58.5

Gene sequence:

>2082_bases
ATGCTGAACCTGTACGACGCGGACGGCAAGATCCAGTTCGACAAGGATCGGGAAGCAGCCCACCAGTACTTTTTGCAGCA
TGTCAATCAGAACACGGTCTTCTTCCATAATCAGGACGAGAAGCTCGACTACCTGATCCGCGAGAATTACTACGAGCGTG
AGGTTCTCGACCAGTACTCGCGCAACTTCGTCAAGACGCTGCTAGACCGCGCCTACGCCAAAAAGTTCCGGTTTCCGACG
TTTTTGGGTGCGTTCAAGTACTACACCTCCTACACGCTGAAAACCTTTGACGGGAAGCGCTATCTGGAGCGCTTCGAGGA
CCGCGTGGTCATGGTGGCGCTAACGTTGGCCGCCGGCGATACCGCACTTGCCGAGCTGCTGGTCGACGAGATCATCGACG
GCCGCTTCCAGCCCGCCACACCGACGTTTTTGAATTCTGGCAAGAAGCAGCGCGGGGAGCCCGTGAGCTGTTTTTTGCTT
CGCGTCGAAGACAACATGGAGTCGATCGGACGGTCGATCAACTCCGCGCTGCAGCTATCCAAGCGTGGCGGGGGAGTGGC
GTTGCTGCTGACCAACATTCGCGAGCACGGCGCGCCGATCAAGAACATCGAGAACCAGTCCTCGGGCGTCATCCCCATCA
TGAAGTTGCTGGAGGATGCGTTCTCCTACGCCAACCAGCTGGGCGCTCGTCAAGGTGCCGGCGCGGTGTACCTGCACGCC
CATCACCCCGACATCTACCGATTCCTGGACACCAAGCGTGAGAACGCCGACGAGAAGATCCGGATCAAGACGCTGAGTCT
GGGGGTGGTGATCCCCGACATCACCTTCGAGTTGGCCAAGCGCAACGATGACATGTACCTGTTCTCGCCCTACGATGTCG
AGCGGGTCTACGGTGTGCCGTTCGCTGACATCTCGGTCACCGAGAAGTACTACGAAATGGTCGATGACGCGCGCATCCGC
AAGACCAAGATCAAGGCACGGGAGTTCTTCCAGACGCTGGCCGAGCTGCAGTTCGAGTCCGGCTACCCCTATATCATGTT
CGAAGACACCGTCAATCGCGCTAATCCAATTGATGGCAAGATCACGCACAGCAACCTGTGCTCGGAGATCCTGCAAGTGT
CTACGCCGTCATTGTTCAACGAGGACTTGTCGTATGCCAAAGTGGGCAAAGACATTTCGTGCAACCTGGGGTCGCTGAAC
ATCGCCAAGACGATGGACTCGCCGGACTTCGCGCAGACGATCGAGGTGGCGATCCGCGCGTTGACCGCGGTGAGCGACCA
AACCCATATCAAGTCGGTGCCCTCAATCGAGCAGGGCAACAACGACTCCCACGCGATCGGGCTAGGACAGATGAACCTGC
ACGGCTACCTGGCCCGGGAACGCATCTTCTACGGATCCGACGAAGGCATCGACTTCACCAACATCTACTTCTATACGGTG
CTGTATCACGCGTTGCGGGCATCCAACCGCATCGCGATCGAACGCGGCACGCACTTCAAGGGTTTCGAGCGGTCCAAGTA
CGCGTCCGGGGAATTCTTCGACAAGTACACCGACCAGATTTGGGAGCCGAAGACCCAGAAGGTACGCCAGCTGTTCGCCG
ACGCCGGCATCCGCATCCCAACGCAGGACGACTGGCGTCGGCTCAAGGAGTCGGTGCAAGCGCACGGCATCTACAACCAG
AACCTGCAGGCGGTGCCGCCGACCGGGTCGATTTCCTACATCAACCATTCGACGTCGTCGATTCACCCGATCGTGTCGAA
GGTCGAGATCCGCAAGGAAGGCAAGATCGGGCGGGTCTACTACCCGGCGCCGTATATGACCAACGACAACCTGGAGTACT
ACGAAGACGCCTACGAGATCGGTTACGAGAAGATCATCGACACCTACGCGGCGGCCACCCAGCATGTGGATCAAGGGCTT
TCGCTGACGTTGTTCTTCAAAGACACCGCCACCACCCGCGACGTGAACAAGGCGCAGATTTACGCCTGGCGCAAGGGGAT
CAAGACGCTGTACTACATCCGGCTGCGGCAGATGGCGTTGGAGGGCACCGAGGTCGAGGGTTGCGTGTCCTGCATGCTGT
AG

Upstream 100 bases:

>100_bases
AGACGTGCCACCAACCGTCATTGCAGAGCCTGTAGCCTCCGGCGCGCACGCCTCTTACTCTGGGGGGCCGGGCGAAACGG
ACTATCACGCGCTGAACGCG

Downstream 100 bases:

>100_bases
CACCGGCGCGCCAGAGTGAAAGTGGCGACGGCTTCGCGGCGTGTCGCGTCGTGAACCTCACATTCAACCAAGCCTGGCGT
GGCCAGTCTGCCCAGGTCAG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYSRNFVKTLLDRAYAKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDTALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLL
RVEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHA
HHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVPFADISVTEKYYEMVDDARIR
KTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGKITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLN
IAKTMDSPDFAQTIEVAIRALTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTV
LYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIPTQDDWRRLKESVQAHGIYNQ
NLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVYYPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGL
SLTLFFKDTATTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML

Sequences:

>Translated_693_residues
MLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYSRNFVKTLLDRAYAKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDTALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLL
RVEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHA
HHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVPFADISVTEKYYEMVDDARIR
KTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGKITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLN
IAKTMDSPDFAQTIEVAIRALTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTV
LYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIPTQDDWRRLKESVQAHGIYNQ
NLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVYYPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGL
SLTLFFKDTATTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
>Mature_693_residues
MLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYSRNFVKTLLDRAYAKKFRFPT
FLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDTALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLL
RVEDNMESIGRSINSALQLSKRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHA
HHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVPFADISVTEKYYEMVDDARIR
KTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGKITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLN
IAKTMDSPDFAQTIEVAIRALTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTV
LYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIPTQDDWRRLKESVQAHGIYNQ
NLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVYYPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGL
SLTLFFKDTATTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family

Homologues:

Organism=Homo sapiens, GI4506749, Length=643, Percent_Identity=26.2830482115086, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1789030, Length=693, Percent_Identity=73.5930735930736, Blast_Score=1085, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=561, Percent_Identity=26.9162210338681, Blast_Score=181, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17555242, Length=643, Percent_Identity=26.7496111975117, Blast_Score=192, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6320914, Length=655, Percent_Identity=26.412213740458, Blast_Score=180, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=26.1467889908257, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17352481, Length=642, Percent_Identity=25.2336448598131, Blast_Score=185, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR1_MYCBO (P0A5W9)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856722.1
- ProteinModelPortal:   P0A5W9
- SMR:   P0A5W9
- EnsemblBacteria:   EBMYCT00000018003
- GeneID:   1093421
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3077c
- GeneTree:   EBGT00050000017700
- HOGENOM:   HBG348953
- ProtClustDB:   PRK08188
- BioCyc:   MBOV233413:MB3077C-MONOMER
- BRENDA:   1.17.4.1
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554
- PANTHER:   PTHR11573
- PRINTS:   PR01183
- TIGRFAMs:   TIGR02506

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N; SSF48168 Ribonucleo_red_N

EC number: =1.17.4.1

Molecular weight: Translated: 79292; Mature: 79292

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00089 RIBORED_LARGE

Important sites: ACT_SITE 397-397 ACT_SITE 399-399 ACT_SITE 401-401 BINDING 172-172 BINDING 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYS
CCEEECCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHH
RNFVKTLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEEEEEEECCCH
TALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLRVEDNMESIGRSINSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHCCCCHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
KRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHA
CCCCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVP
CCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHHCCCCEEEECCCCCHHHCCCC
FADISVTEKYYEMVDDARIRKTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
ITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRA
EEHHHHHHHHHHHCCCHHHHHCCCHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
LTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTV
HHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHEEEECCCCCCCHHHHHHHHH
LYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIP
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
TQDDWRRLKESVQAHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVY
CHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
YPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGLSLTLFFKDTATTRDVNKAQI
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH
YAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLNLYDADGKIQFDKDREAAHQYFLQHVNQNTVFFHNQDEKLDYLIRENYYEREVLDQYS
CCEEECCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHH
RNFVKTLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHCCEEEEEEEECCCH
TALAELLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLRVEDNMESIGRSINSALQLS
HHHHHHHHHHHHCCCCCCCCCHHHCCCCHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
KRGGGVALLLTNIREHGAPIKNIENQSSGVIPIMKLLEDAFSYANQLGARQGAGAVYLHA
CCCCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
HHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERVYGVP
CCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCHHHHHHCCCCEEEECCCCCHHHCCCC
FADISVTEKYYEMVDDARIRKTKIKAREFFQTLAELQFESGYPYIMFEDTVNRANPIDGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
ITHSNLCSEILQVSTPSLFNEDLSYAKVGKDISCNLGSLNIAKTMDSPDFAQTIEVAIRA
EEHHHHHHHHHHHCCCHHHHHCCCHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
LTAVSDQTHIKSVPSIEQGNNDSHAIGLGQMNLHGYLARERIFYGSDEGIDFTNIYFYTV
HHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHEEEECCCCCCCHHHHHHHHH
LYHALRASNRIAIERGTHFKGFERSKYASGEFFDKYTDQIWEPKTQKVRQLFADAGIRIP
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
TQDDWRRLKESVQAHGIYNQNLQAVPPTGSISYINHSTSSIHPIVSKVEIRKEGKIGRVY
CHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEE
YPAPYMTNDNLEYYEDAYEIGYEKIIDTYAAATQHVDQGLSLTLFFKDTATTRDVNKAQI
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHH
YAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972