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The map label for this gene is ddl [H]

Identifier: 148662831

GI number: 148662831

Start: 3348965

End: 3350086

Strand: Reverse

Name: ddl [H]

Synonym: MRA_3010

Alternate gene names: 148662831

Gene position: 3350086-3348965 (Counterclockwise)

Preceding gene: 148662832

Following gene: 148662829

Centisome position: 75.79

GC content: 67.47

Gene sequence:

>1122_bases
GTGAGTGCTAACGACCGGCGTGATCGGCGTGTCCGCGTTGCCGTCGTGTTCGGCGGGCGCAGCAACGAGCACGCCATCTC
GTGTGTGTCCGCCGGCAGCATCCTGCGCAACCTGGACTCGCGGCGGTTCGACGTGATCGCGGTGGGTATCACCCCGGCAG
GTTCGTGGGTGCTCACCGACGCCAACCCCGACGCCCTGACGATCACCAACCGGGAGCTTCCTCAGGTCAAATCAGGATCG
GGCACCGAGCTGGCGCTGCCGGCCGATCCGCGGCGTGGTGGCCAGTTGGTGTCGCTGCCGCCCGGGGCCGGCGAGGTTCT
GGAGTCGGTCGACGTGGTGTTCCCGGTACTGCACGGCCCGTACGGCGAGGACGGCACGATCCAGGGACTGCTCGAACTCG
CCGGGGTGCCCTACGTGGGCGCCGGTGTGCTGGCCAGTGCCGTCGGCATGGACAAGGAGTTCACCAAGAAGCTGCTCGCC
GCCGATGGACTTCCGGTGGGTGCGTACGCGGTGCTGCGTCCGCCGCGGTCGACACTGCACCGCCAGGAGTGCGAACGGCT
GGGCTTACCGGTGTTCGTCAAACCCGCCCGAGGCGGCTCGTCGATCGGTGTTAGCCGGGTGTCGAGTTGGGATCAACTGC
CCGCCGCGGTCGCGCGGGCCCGCCGGCATGACCCTAAGGTCATCGTCGAGGCCGCGATCAGCGGCCGCGAGCTGGAATGC
GGTGTGCTCGAAATGCCGGACGGCACACTGGAAGCCAGCACGCTGGGGGAGATCCGGGTGGCCGGGGTGCGGGGACGCGA
GGACTCTTTCTACGACTTCGCAACCAAGTATCTCGACGACGCAGCCGAATTGGACGTGCCCGCCAAGGTCGATGACCAGG
TCGCAGAGGCGATTCGTCAGCTGGCGATCCGGGCGTTCGCGGCTATCGACTGCCGGGGTCTGGCCAGGGTGGACTTCTTC
CTCACCGACGACGGTCCGGTGATCAACGAGATCAACACGATGCCGGGATTCACCACGATCTCGATGTACCCGCGGATGTG
GGCGGCCAGCGGTGTCGACTATCCGACCCTGCTGGCGACGATGATCGAGACGACATTGGCCCGCGGCGTGGGCCTGCACT
AG

Upstream 100 bases:

>100_bases
GTCGCCGCACCAAGCCCGAATGAGCCTCGCACGGGCCGGCACGACCGAAAACTGCAGCTCGTGGGAGCCCGTCTGCCTGA
CCCGGTAGCCTCTGCAGGTT

Downstream 100 bases:

>100_bases
CCCGATGGTGCCGACCCGCCGCGCCCGGCGCTAGCGCCGCGCTTGCGATCGGCACTTAGCCCGATGGTGCCGACCCGCCG
CGCCCGGCGCTAGCGCCGCG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGS
GTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLA
ADGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFF
LTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETTLARGVGLH

Sequences:

>Translated_373_residues
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGS
GTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLA
ADGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFF
LTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETTLARGVGLH
>Mature_372_residues
SANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTDANPDALTITNRELPQVKSGSG
TELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAA
DGLPVGAYAVLRPPRSTLHRQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELECG
VLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQLAIRAFAAIDCRGLARVDFFL
TDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLATMIETTLARGVGLH

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=43.4065934065934, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1786280, Length=258, Percent_Identity=36.4341085271318, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39711; Mature: 39580

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTD
CCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEEECCCCCEEEEC
ANPDALTITNRELPQVKSGSGTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGP
CCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
YGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAADGLPVGAYAVLRPPRSTLH
CCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHH
RQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
HHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCEECC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQ
CEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAIRAFAAIDCRGLARVDFFLTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLAT
HHHHHHHHHCCCCCEEEEEEECCCCCEEHHCCCCCCEEEEECCHHHHHCCCCCHHHHHHH
MIETTLARGVGLH
HHHHHHHHCCCCC
>Mature Secondary Structure 
SANDRRDRRVRVAVVFGGRSNEHAISCVSAGSILRNLDSRRFDVIAVGITPAGSWVLTD
CCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEEECCCCCEEEEC
ANPDALTITNRELPQVKSGSGTELALPADPRRGGQLVSLPPGAGEVLESVDVVFPVLHGP
CCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
YGEDGTIQGLLELAGVPYVGAGVLASAVGMDKEFTKKLLAADGLPVGAYAVLRPPRSTLH
CCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHH
RQECERLGLPVFVKPARGGSSIGVSRVSSWDQLPAAVARARRHDPKVIVEAAISGRELEC
HHHHHHCCCCEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCEECC
GVLEMPDGTLEASTLGEIRVAGVRGREDSFYDFATKYLDDAAELDVPAKVDDQVAEAIRQ
CEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LAIRAFAAIDCRGLARVDFFLTDDGPVINEINTMPGFTTISMYPRMWAASGVDYPTLLAT
HHHHHHHHHCCCCCEEEEEEECCCCCEEHHCCCCCCEEEEECCHHHHHCCCCCHHHHHHH
MIETTLARGVGLH
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA