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The map label for this gene is dinF [H]

Identifier: 148662677

GI number: 148662677

Start: 3154301

End: 3155620

Strand: Reverse

Name: dinF [H]

Synonym: MRA_2859

Alternate gene names: 148662677

Gene position: 3155620-3154301 (Counterclockwise)

Preceding gene: 148662678

Following gene: 148662676

Centisome position: 71.39

GC content: 68.86

Gene sequence:

>1320_bases
TTGAGCCAGGTGGGGCACCGCGCGGGCGGCCGTCAGATCGCGCAGCTGGCGTTGCCCGCGCTGGGCGTGCTGGCCGCCGA
GCCGCTGTACCTGCTGTTCGACATCGCCGTGGTGGGCCGGCTGGGGGCGATATCGCTGGCGGGTCTGGCTATCGGCAGTC
TGGTGCTCGGCCTGGTTGGTTCCCAGGCGACGTTTTTGTCCTACGGCACCACAGCGCGCGCGGCGCGTCGTTACGGTGCC
GGCAACCGGGTGGCCGCGGTCACCGAAGGTGTACAGGCGACCTGGTTGGCGTTGGGTCTGGGCGCGTTGGTCGTCGTCGT
GGTGGAAGCCACCGCGACGCCGCTGGTGTCGGCGATCGCTTCTGGCGATGGCATCACCGCGGCGGCCTTGCCGTGGCTGC
GGATCGCGATCCTGGGCACGCCGGCGATCCTGGTCTCGCTCGCCGGCAACGGCTGGCTGCGCGGCGTGCAGGACACCGTG
CGACCGCTGCGGTATGTGGTCGCCGGCTTCGGGTCGTCGGCTCTGCTGTGCCCGCTGCTGGTTTACGGTTGGCTGGGGTT
GCCCCGCTGGGGGTTAACCGGCTCGGCGGTGGCCAATCTGGTGGGGCAGTGGCTGGCGGCGCTGCTGTTTGCCGGTGCGT
TACTGGCCGAGCGGGTGTCGCTGCGGCCGGACCGCGCCGTGCTGGGCGCCCAGCTGATGATGGCGCGGGACCTGATCGTG
CGGACCCTGGCCTTCCAAGTTTGTTATGTCTCGGCTGCGGCGGTGGCCGCGAGGTTCGGCGCTGCCGCGCTCGCGGCCCA
CCAGGTCGTGTTGCAGTTGTGGGGTCTTCTTGCGCTGGTTCTTGATTCGCTAGCGATTGCGGCGCAGTCGCTGGTCGGTG
CCGCGTTGGGTGCCGGTGATGCCGGGCACGCTAAGGCGGTGGCATGGCGGGTGACGGCGTTTTCGCTGTTGGCGGCGGGA
ATTTTGGCAGCGGCGTTAGGGCTAGGCTCCTCGGTGCTGCCCGGGCTATTCACCGACGATCGATCGGTACTCGCCGCGAT
CGGGGTGCCGTGGTGGTTCATGGTAGTCCAATTGCCTTTTGCGGGAATTGTTTTCGCAGTTGACGGGGTGCTGCTGGGCG
CGGGCGACGCCGCGTTCATGCGGACGGCGACGGTGGCGAGTGCGTTGGTGGGCTTTTTGCCGCTTGTCTGGTTGTCGCTG
GCGTATGGCTGGGGGCTGGCGGGTATCTGGTCGGGACTGGGCACGTTTATCGTGCTGCGGCTGATATTCGTCGGATGGCG
GGCGTACAGCGGTCGGTGGGCGGTGACGGGCGCGGCGTAG

Upstream 100 bases:

>100_bases
GTTCGGTGGCGGTGGTCACCGGCTGGCCGCGGGGTATACGACCACCGGCTCGATCGACGACGCTGTGGCGTCGTTGCGCG
CGGCGCTTGGTTAGCCGCCG

Downstream 100 bases:

>100_bases
CTGGCCGACATTGCCGTGACGGCGGTGATTGGTTCATCGACCGTGGCGGCAAACTCGCCGACAGAATACGGAGCTACACT
GCGCCGATGGCGGCGCCGCA

Product: putative DNA-damage-inducible protein F

Products: NA

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSQVGHRAGGRQIAQLALPALGVLAAEPLYLLFDIAVVGRLGAISLAGLAIGSLVLGLVGSQATFLSYGTTARAARRYGA
GNRVAAVTEGVQATWLALGLGALVVVVVEATATPLVSAIASGDGITAAALPWLRIAILGTPAILVSLAGNGWLRGVQDTV
RPLRYVVAGFGSSALLCPLLVYGWLGLPRWGLTGSAVANLVGQWLAALLFAGALLAERVSLRPDRAVLGAQLMMARDLIV
RTLAFQVCYVSAAAVAARFGAAALAAHQVVLQLWGLLALVLDSLAIAAQSLVGAALGAGDAGHAKAVAWRVTAFSLLAAG
ILAAALGLGSSVLPGLFTDDRSVLAAIGVPWWFMVVQLPFAGIVFAVDGVLLGAGDAAFMRTATVASALVGFLPLVWLSL
AYGWGLAGIWSGLGTFIVLRLIFVGWRAYSGRWAVTGAA

Sequences:

>Translated_439_residues
MSQVGHRAGGRQIAQLALPALGVLAAEPLYLLFDIAVVGRLGAISLAGLAIGSLVLGLVGSQATFLSYGTTARAARRYGA
GNRVAAVTEGVQATWLALGLGALVVVVVEATATPLVSAIASGDGITAAALPWLRIAILGTPAILVSLAGNGWLRGVQDTV
RPLRYVVAGFGSSALLCPLLVYGWLGLPRWGLTGSAVANLVGQWLAALLFAGALLAERVSLRPDRAVLGAQLMMARDLIV
RTLAFQVCYVSAAAVAARFGAAALAAHQVVLQLWGLLALVLDSLAIAAQSLVGAALGAGDAGHAKAVAWRVTAFSLLAAG
ILAAALGLGSSVLPGLFTDDRSVLAAIGVPWWFMVVQLPFAGIVFAVDGVLLGAGDAAFMRTATVASALVGFLPLVWLSL
AYGWGLAGIWSGLGTFIVLRLIFVGWRAYSGRWAVTGAA
>Mature_438_residues
SQVGHRAGGRQIAQLALPALGVLAAEPLYLLFDIAVVGRLGAISLAGLAIGSLVLGLVGSQATFLSYGTTARAARRYGAG
NRVAAVTEGVQATWLALGLGALVVVVVEATATPLVSAIASGDGITAAALPWLRIAILGTPAILVSLAGNGWLRGVQDTVR
PLRYVVAGFGSSALLCPLLVYGWLGLPRWGLTGSAVANLVGQWLAALLFAGALLAERVSLRPDRAVLGAQLMMARDLIVR
TLAFQVCYVSAAAVAARFGAAALAAHQVVLQLWGLLALVLDSLAIAAQSLVGAALGAGDAGHAKAVAWRVTAFSLLAAGI
LAAALGLGSSVLPGLFTDDRSVLAAIGVPWWFMVVQLPFAGIVFAVDGVLLGAGDAAFMRTATVASALVGFLPLVWLSLA
YGWGLAGIWSGLGTFIVLRLIFVGWRAYSGRWAVTGAA

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=448, Percent_Identity=27.9017857142857, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI226510954, Length=444, Percent_Identity=22.972972972973, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 44756; Mature: 44625

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVGHRAGGRQIAQLALPALGVLAAEPLYLLFDIAVVGRLGAISLAGLAIGSLVLGLVG
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SQATFLSYGTTARAARRYGAGNRVAAVTEGVQATWLALGLGALVVVVVEATATPLVSAIA
CCHHHHCCCCHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGDGITAAALPWLRIAILGTPAILVSLAGNGWLRGVQDTVRPLRYVVAGFGSSALLCPLL
CCCCCHHHHHHHHHHHHHCCHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
VYGWLGLPRWGLTGSAVANLVGQWLAALLFAGALLAERVSLRPDRAVLGAQLMMARDLIV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RTLAFQVCYVSAAAVAARFGAAALAAHQVVLQLWGLLALVLDSLAIAAQSLVGAALGAGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGHAKAVAWRVTAFSLLAAGILAAALGLGSSVLPGLFTDDRSVLAAIGVPWWFMVVQLPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHCCHHHHHHHHHHH
AGIVFAVDGVLLGAGDAAFMRTATVASALVGFLPLVWLSLAYGWGLAGIWSGLGTFIVLR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LIFVGWRAYSGRWAVTGAA
HHHHHHHHCCCCEEEECCC
>Mature Secondary Structure 
SQVGHRAGGRQIAQLALPALGVLAAEPLYLLFDIAVVGRLGAISLAGLAIGSLVLGLVG
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SQATFLSYGTTARAARRYGAGNRVAAVTEGVQATWLALGLGALVVVVVEATATPLVSAIA
CCHHHHCCCCHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGDGITAAALPWLRIAILGTPAILVSLAGNGWLRGVQDTVRPLRYVVAGFGSSALLCPLL
CCCCCHHHHHHHHHHHHHCCHHHHEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
VYGWLGLPRWGLTGSAVANLVGQWLAALLFAGALLAERVSLRPDRAVLGAQLMMARDLIV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RTLAFQVCYVSAAAVAARFGAAALAAHQVVLQLWGLLALVLDSLAIAAQSLVGAALGAGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGHAKAVAWRVTAFSLLAAGILAAALGLGSSVLPGLFTDDRSVLAAIGVPWWFMVVQLPF
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHCCHHHHHHHHHHH
AGIVFAVDGVLLGAGDAAFMRTATVASALVGFLPLVWLSLAYGWGLAGIWSGLGTFIVLR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LIFVGWRAYSGRWAVTGAA
HHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]