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The map label for this gene is ruvC

Identifier: 148662433

GI number: 148662433

Start: 2936785

End: 2937351

Strand: Reverse

Name: ruvC

Synonym: MRA_2623

Alternate gene names: 148662433

Gene position: 2937351-2936785 (Counterclockwise)

Preceding gene: 148662443

Following gene: 148662432

Centisome position: 66.46

GC content: 66.31

Gene sequence:

>567_bases
GTGCGGGTGATGGGTGTCGATCCCGGGTTGACCCGATGCGGGCTGTCGCTTATCGAGAGTGGGCGTGGTCGGCAGCTCAC
CGCGCTGGATGTCGACGTGGTGCGCACACCGTCGGATGCGGCCTTGGCGCAGCGCCTGTTGGCCATCAGCGATGCCGTCG
AGCACTGGCTGGACACCCATCATCCGGAGGTGGTGGCTATCGAACGGGTGTTCTCTCAGCTCAACGTGACCACGGTGATG
GGCACCGCGCAGGCCGGCGGCGTGATCGCCCTGGCGGCGGCCAAACGTGGTGTCGACGTGCATTTCCATACCCCCAGCGA
GGTCAAGGCGGCGGTCACTGGCAACGGTTCCGCAGACAAGGCTCAGGTCACCGCGATGGTCACCAAAATCCTTGCGCTGC
AAGCTAAACCGACACCGGCCGACGCGGCCGACGCTTTGGCGCTGGCGATCTGTCACTGTTGGCGGGCGCCGACGATCGCC
CGGATGGCTGAGGCCACGTCGAGGGCGGAAGCACGAGCAGCGCAGCAGCGTCATGCGTACCTAGCCAAGCTGAAGGCCGC
CCGATGA

Upstream 100 bases:

>100_bases
ATTTACCATCGCACGTTCCATAGGCGTGTCCTGCGCGGGATGTCGGGACGATCCGCTAGCGTATCGAACGATTGTTCGGA
AATGGCTGAGGGAGCGTGCG

Downstream 100 bases:

>100_bases
TCGCCTCGGTCCGCGGTGAGGTGCTCGAGGTGGCGCTTGACCATGTGGTGATCGAGGCCGCCGGTGTGGGCTACCGAGTG
AACGCGACACCGGCGACGCT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MRVMGVDPGLTRCGLSLIESGRGRQLTALDVDVVRTPSDAALAQRLLAISDAVEHWLDTHHPEVVAIERVFSQLNVTTVM
GTAQAGGVIALAAAKRGVDVHFHTPSEVKAAVTGNGSADKAQVTAMVTKILALQAKPTPADAADALALAICHCWRAPTIA
RMAEATSRAEARAAQQRHAYLAKLKAAR

Sequences:

>Translated_188_residues
MRVMGVDPGLTRCGLSLIESGRGRQLTALDVDVVRTPSDAALAQRLLAISDAVEHWLDTHHPEVVAIERVFSQLNVTTVM
GTAQAGGVIALAAAKRGVDVHFHTPSEVKAAVTGNGSADKAQVTAMVTKILALQAKPTPADAADALALAICHCWRAPTIA
RMAEATSRAEARAAQQRHAYLAKLKAAR
>Mature_188_residues
MRVMGVDPGLTRCGLSLIESGRGRQLTALDVDVVRTPSDAALAQRLLAISDAVEHWLDTHHPEVVAIERVFSQLNVTTVM
GTAQAGGVIALAAAKRGVDVHFHTPSEVKAAVTGNGSADKAQVTAMVTKILALQAKPTPADAADALALAICHCWRAPTIA
RMAEATSRAEARAAQQRHAYLAKLKAAR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=163, Percent_Identity=38.6503067484663, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_MYCBO (P66761)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856271.1
- ProteinModelPortal:   P66761
- SMR:   P66761
- EnsemblBacteria:   EBMYCT00000016990
- GeneID:   1092007
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2625c
- GeneTree:   EBGT00050000016429
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   MBOV233413:MB2625C-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19786; Mature: 19786

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMGVDPGLTRCGLSLIESGRGRQLTALDVDVVRTPSDAALAQRLLAISDAVEHWLDTH
CEEECCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
HPEVVAIERVFSQLNVTTVMGTAQAGGVIALAAAKRGVDVHFHTPSEVKAAVTGNGSADK
CCCEEHHHHHHHHHCHHHEEECCCCCCEEEEEECCCCCEEEEECCHHHEEEEECCCCCCH
AQVTAMVTKILALQAKPTPADAADALALAICHCWRAPTIARMAEATSRAEARAAQQRHAY
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LAKLKAAR
HHHHHCCC
>Mature Secondary Structure
MRVMGVDPGLTRCGLSLIESGRGRQLTALDVDVVRTPSDAALAQRLLAISDAVEHWLDTH
CEEECCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
HPEVVAIERVFSQLNVTTVMGTAQAGGVIALAAAKRGVDVHFHTPSEVKAAVTGNGSADK
CCCEEHHHHHHHHHCHHHEEECCCCCCEEEEEECCCCCEEEEECCHHHEEEEECCCCCCH
AQVTAMVTKILALQAKPTPADAADALALAICHCWRAPTIARMAEATSRAEARAAQQRHAY
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LAKLKAAR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972