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The map label for this gene is 148662319

Identifier: 148662319

GI number: 148662319

Start: 2802987

End: 2804462

Strand: Reverse

Name: 148662319

Synonym: MRA_2509

Alternate gene names: NA

Gene position: 2804462-2802987 (Counterclockwise)

Preceding gene: 148662320

Following gene: 148662318

Centisome position: 63.45

GC content: 66.19

Gene sequence:

>1476_bases
ATGGCTGAGTCCGGCGAGTCCCCCAGGTTGTCCGACGAGCTTGGGCCGGTCGACTATTTGATGCATCGGGGCGAGGCAAA
TCCGCGGACTCGTTCGGGGATCATGGCGCTGGAACTGCTGGACGGCACGCCGGACTGGGATCGCTTCCGGACCCGATTCG
AAAACGCCTCGCGACGGGTGTTGCGGCTGCGGCAGAAGGTGGTGGTGCCGACCTTGCCGACCGCGGCCCCGCGCTGGGTG
GTGGATCCGGACTTCAATCTGGACTTCCATGTGCGTCGGGTGCGTGTGTCCGGACCCGCCACGTTGCGCGAAGTGCTCGA
TCTCGCCGAGGTGATTCTGCAGTCGCCGCTGGACATATCGCGCCCGCTGTGGACGGCCACCCTGGTGGAGGGTATGGCTG
ACGGCAGAGCGGCGATGCTGCTGCACGTCAGCCATGCGGTCACCGACGGTGTCGGCGGCGTGGAGATGTTCGCGCAGATC
TATGACCTCGAGCGCGATCCACCACCTAGGTCGACGCCGCCGCAACCCATTCCGGAGGATCTGTCCCCCAATGACCTGAT
GCGCCGGGGCATCAACCACCTGCCAATTGCTGTCGTCGGCGGTGTCCTGGACGCGCTGTCCGGGGCGGTATCGATGGCCG
GGCGGGCTGTTCTGGAACCGGTGTCCACCGTGTCGGGGATCTTGGGCTACGCCAGGTCGGGCATCCGGGTGCTGAACCGA
GCTGCTGAACCCTCACCCCTACTGCGCCGGCGCAGCCTGACCACCCGTACCGAGGCCATCGACATCCGGCTCGCCGACCT
GCACAAGGCCGCAAAGGCCGGCGGCGGGTCGATCAACGACGCCTACCTTGCGGGTCTGTGTGGCGCGCTGCGCCGCTACC
ACGAGGCCCTGGGCGTGCCGATCAGCACGCTGCCGATGGCGGTGCCGGTCAACCTGCGGGCCGAGGGCGATGCGGCCGGC
GGCAACCAGTTCACCGGTGTCAACCTGGCGGCGCCGGTGGGCACTATCGATCCGGTGGCTCGGATGAAGAAGATCCGGGC
GCAGATGACCCAGCGTCGCGACGAGCCCGCGATGAACATCATCGGTTCCATTGCACCGGTGCTGAGTGTCTTGCCGACGG
CCGTGCTGGAGGGGATCACCGGTTCGGTGATCGGTTCCGATGTGCAAGCCAGCAATGTTCCGGTCTACCCAGGGGATACC
TACCTTGCCGGCGCGAAGATACTGCGGCAGTACGGTATTGGACCGTTGCCCGGTGTGGCGATGATGGTAGTGCTAATTTC
CCGGGGCGGGTGGTGCACCGTCACGGTGCGCTACGACAGGGCGTCGGTGCGAAACGACGAGTTGTTCGCTCAATGCCTGC
AGGCGGGTTTCGACGAAATCCTTGCGCTGGCCGGTGGTCCGGCACCGCGTGTGCTGCCGGCGTCGTTCGATACTCAAGGA
GCCGGTTCAGTGCCTCGATCGGTGTCGGGCTCATGA

Upstream 100 bases:

>100_bases
GCACTGTGGTGCGTGGCGGTGGCGGGTTTGAAGCAGGAGGTCTGATCGGCGGTGACAGAGTTTGTGCGTCGAGTTGCCCA
ACCCGCGCGGGGTCGTGGCC

Downstream 100 bases:

>100_bases
GCGCCGCTGACGAGCAGGGGGAAGAGCGGGCGACGCGGAAGTCGGCGCCGGATTTGCGCCTACCCGGTTCGGTAGCCGAG
ATTCTGGCCAGTCCTGCCGG

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv2484c/MT2557

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRVLRLRQKVVVPTLPTAAPRWV
VDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQI
YDLERDPPPRSTPPQPIPEDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVLNR
AAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVPISTLPMAVPVNLRAEGDAAG
GNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDT
YLAGAKILRQYGIGPLPGVAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDTQG
AGSVPRSVSGS

Sequences:

>Translated_491_residues
MAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRVLRLRQKVVVPTLPTAAPRWV
VDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQI
YDLERDPPPRSTPPQPIPEDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVLNR
AAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVPISTLPMAVPVNLRAEGDAAG
GNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDT
YLAGAKILRQYGIGPLPGVAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDTQG
AGSVPRSVSGS
>Mature_490_residues
AESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRVLRLRQKVVVPTLPTAAPRWVV
DPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDISRPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQIY
DLERDPPPRSTPPQPIPEDLSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVLNRA
AEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVPISTLPMAVPVNLRAEGDAAGG
NQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNIIGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDTY
LAGAKILRQYGIGPLPGVAMMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDTQGA
GSVPRSVSGS

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2484_MYCTU (O53209)

Other databases:

- EMBL:   BX842580
- EMBL:   AE000516
- PIR:   C70868
- RefSeq:   NP_217000.1
- RefSeq:   NP_337047.1
- ProteinModelPortal:   O53209
- EnsemblBacteria:   EBMYCT00000001989
- EnsemblBacteria:   EBMYCT00000071729
- GeneID:   888623
- GeneID:   925768
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2557
- KEGG:   mtu:Rv2484c
- TIGR:   MT2557
- TubercuList:   Rv2484c
- GeneTree:   EBGT00050000015247
- HOGENOM:   HBG567860
- OMA:   RSEAIDI
- ProtClustDB:   CLSK791877
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 52310; Mature: 52179

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRV
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LRLRQKVVVPTLPTAAPRWVVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDIS
HHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCC
RPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQIYDLERDPPPRSTPPQPIPED
CCHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVLNR
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVP
CCCCCHHHHHHHCCHHHHHEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ISTLPMAVPVNLRAEGDAAGGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNI
HHHCCEEECEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
IGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDTYLAGAKILRQYGIGPLPGVA
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
MMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDTQG
HHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
AGSVPRSVSGS
CCCCCCCCCCC
>Mature Secondary Structure 
AESGESPRLSDELGPVDYLMHRGEANPRTRSGIMALELLDGTPDWDRFRTRFENASRRV
CCCCCCCCCCCCCCHHHHHHHCCCCCCCHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
LRLRQKVVVPTLPTAAPRWVVDPDFNLDFHVRRVRVSGPATLREVLDLAEVILQSPLDIS
HHHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCC
RPLWTATLVEGMADGRAAMLLHVSHAVTDGVGGVEMFAQIYDLERDPPPRSTPPQPIPED
CCHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LSPNDLMRRGINHLPIAVVGGVLDALSGAVSMAGRAVLEPVSTVSGILGYARSGIRVLNR
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEPSPLLRRRSLTTRTEAIDIRLADLHKAAKAGGGSINDAYLAGLCGALRRYHEALGVP
CCCCCHHHHHHHCCHHHHHEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
ISTLPMAVPVNLRAEGDAAGGNQFTGVNLAAPVGTIDPVARMKKIRAQMTQRRDEPAMNI
HHHCCEEECEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHH
IGSIAPVLSVLPTAVLEGITGSVIGSDVQASNVPVYPGDTYLAGAKILRQYGIGPLPGVA
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
MMVVLISRGGWCTVTVRYDRASVRNDELFAQCLQAGFDEILALAGGPAPRVLPASFDTQG
HHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
AGSVPRSVSGS
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036