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The map label for this gene is uppS

Identifier: 148662193

GI number: 148662193

Start: 2654546

End: 2655436

Strand: Reverse

Name: uppS

Synonym: MRA_2385

Alternate gene names: 148662193

Gene position: 2655436-2654546 (Counterclockwise)

Preceding gene: 148662194

Following gene: 148662192

Centisome position: 60.08

GC content: 64.42

Gene sequence:

>891_bases
GTGGCTAGGGATGCACGGAAGCGGACGTCCAGCAACTTCCCGCAGCTGCCTCCGGCCCCCGACGACTACCCGACCTTTCC
TGATACGTCGACGTGGCCCGTCGTCTTCCCGGAGTTGCCGGCAGCGCCCTACGGAGGCCCCTGCCGGCCACCGCAGCACA
CTTCGAAAGCGGCCGCGCCACGAATACCGGCCGACCGGCTGCCCAACCATGTCGCCATTGTCATGGACGGCAATGGCCGC
TGGGCCACCCAACGTGGGCTGGCCCGCACCGAAGGTCACAAGATGGGCGAGGCGGTGGTGATCGACATCGCTTGCGGTGC
TATCGAACTTGGGATCAAGTGGCTCAGCCTCTACGCCTTCTCCACGGAGAACTGGAAGCGTTCGCCCGAGGAAGTCCGCT
TCCTGATGGGCTTCAACCGCGACGTGGTGCGACGGCGCCGAGACACCCTAAAGAAGCTAGGGGTCCGGATCCGGTGGGTG
GGTTCACGACCACGGCTGTGGCGTAGCGTCATCAATGAGTTGGCGGTCGCGGAGGAGATGACCAAAAGCAACGACGTCAT
CACCATCAACTATTGCGTCAACTACGGCGGACGCACCGAAATCACCGAAGCCACCCGCGAAATCGCTCGTGAGGTCGCCG
CGGGCAGGCTCAACCCGGAGCGGATCACCGAATCCACGATCGCCCGCCACCTGCAGCGACCCGACATTCCCGACGTTGAC
CTCTTCCTGCGGACCTCGGGTGAGCAGCGCTCCAGCAACTTCATGCTGTGGCAGGCGGCCTACGCTGAATACATATTCCA
GGACAAGCTCTGGCCCGACTATGACCGCCGCGACTTGTGGGCGGCCTGCGAGGAATACGCGTCGCGCACTCGGCGGTTCG
GGAGCGCCTAG

Upstream 100 bases:

>100_bases
GAACGGCAGCTGAAAACGTTGCCGCTGGTGGAGCGGTTCTACCAGGCCGATCGATCGGTTGCCGAACGTCGCGCCGCGCT
GATCGGGCAGGATATAGCCG

Downstream 100 bases:

>100_bases
TGCCATCCCTACCGGACCGGCTGGCATCGATACTGCGCGATGTCCTTCCTGCCGAGGAGGAACCCGACGGCGCGCTGACG
GTTCGCCATGACGGTACGTT

Product: undecaprenyl pyrophosphate synthase

Products: NA

Alternate protein names: UPP synthase; Di-trans,poly-cis-decaprenylcistransferase; Undecaprenyl diphosphate synthase; UDS

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MARDARKRTSSNFPQLPPAPDDYPTFPDTSTWPVVFPELPAAPYGGPCRPPQHTSKAAAPRIPADRLPNHVAIVMDGNGR
WATQRGLARTEGHKMGEAVVIDIACGAIELGIKWLSLYAFSTENWKRSPEEVRFLMGFNRDVVRRRRDTLKKLGVRIRWV
GSRPRLWRSVINELAVAEEMTKSNDVITINYCVNYGGRTEITEATREIAREVAAGRLNPERITESTIARHLQRPDIPDVD
LFLRTSGEQRSSNFMLWQAAYAEYIFQDKLWPDYDRRDLWAACEEYASRTRRFGSA

Sequences:

>Translated_296_residues
MARDARKRTSSNFPQLPPAPDDYPTFPDTSTWPVVFPELPAAPYGGPCRPPQHTSKAAAPRIPADRLPNHVAIVMDGNGR
WATQRGLARTEGHKMGEAVVIDIACGAIELGIKWLSLYAFSTENWKRSPEEVRFLMGFNRDVVRRRRDTLKKLGVRIRWV
GSRPRLWRSVINELAVAEEMTKSNDVITINYCVNYGGRTEITEATREIAREVAAGRLNPERITESTIARHLQRPDIPDVD
LFLRTSGEQRSSNFMLWQAAYAEYIFQDKLWPDYDRRDLWAACEEYASRTRRFGSA
>Mature_295_residues
ARDARKRTSSNFPQLPPAPDDYPTFPDTSTWPVVFPELPAAPYGGPCRPPQHTSKAAAPRIPADRLPNHVAIVMDGNGRW
ATQRGLARTEGHKMGEAVVIDIACGAIELGIKWLSLYAFSTENWKRSPEEVRFLMGFNRDVVRRRRDTLKKLGVRIRWVG
SRPRLWRSVINELAVAEEMTKSNDVITINYCVNYGGRTEITEATREIAREVAAGRLNPERITESTIARHLQRPDIPDVDL
FLRTSGEQRSSNFMLWQAAYAEYIFQDKLWPDYDRRDLWAACEEYASRTRRFGSA

Specific function: Generates undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide

COG id: COG0020

COG function: function code I; Undecaprenyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPP synthase family

Homologues:

Organism=Homo sapiens, GI45580742, Length=232, Percent_Identity=37.0689655172414, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI45580738, Length=232, Percent_Identity=37.0689655172414, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1786371, Length=226, Percent_Identity=42.0353982300885, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI71993029, Length=230, Percent_Identity=34.3478260869565, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319474, Length=213, Percent_Identity=37.0892018779343, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323748, Length=218, Percent_Identity=32.5688073394495, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI18857969, Length=209, Percent_Identity=35.8851674641148, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UPPS_MYCTU (P60479)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   H70585
- RefSeq:   NP_216877.1
- RefSeq:   NP_336910.1
- PDB:   2VG2
- PDB:   2VG3
- PDB:   2VG4
- PDBsum:   2VG2
- PDBsum:   2VG3
- PDBsum:   2VG4
- ProteinModelPortal:   P60479
- SMR:   P60479
- EnsemblBacteria:   EBMYCT00000003474
- EnsemblBacteria:   EBMYCT00000071422
- GeneID:   888964
- GeneID:   925908
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2430
- KEGG:   mtu:Rv2361c
- TIGR:   MT2430
- TubercuList:   Rv2361c
- GeneTree:   EBGT00050000016292
- HOGENOM:   HBG627837
- OMA:   RVRWAGR
- ProtClustDB:   PRK14827
- BioCyc:   MetaCyc:MONOMER-15221
- BRENDA:   2.5.1.31
- GO:   GO:0005829
- GO:   GO:0040007
- HAMAP:   MF_01139
- InterPro:   IPR001441
- InterPro:   IPR018520
- Gene3D:   G3DSA:3.40.1180.10
- PANTHER:   PTHR10291
- TIGRFAMs:   TIGR00055

Pfam domain/function: PF01255 Prenyltransf; SSF64005 UPP_synth

EC number: =2.5.1.31

Molecular weight: Translated: 33792; Mature: 33660

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS01066 UPP_SYNTHASE

Important sites: ACT_SITE 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDARKRTSSNFPQLPPAPDDYPTFPDTSTWPVVFPELPAAPYGGPCRPPQHTSKAAAP
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCC
RIPADRLPNHVAIVMDGNGRWATQRGLARTEGHKMGEAVVIDIACGAIELGIKWLSLYAF
CCCHHHCCCEEEEEECCCCCCHHHCCCHHHCCCCCCCEEEEEEECCHHHHHHHHHHHEEE
STENWKRSPEEVRFLMGFNRDVVRRRRDTLKKLGVRIRWVGSRPRLWRSVINELAVAEEM
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
TKSNDVITINYCVNYGGRTEITEATREIAREVAAGRLNPERITESTIARHLQRPDIPDVD
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCE
LFLRTSGEQRSSNFMLWQAAYAEYIFQDKLWPDYDRRDLWAACEEYASRTRRFGSA
EEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ARDARKRTSSNFPQLPPAPDDYPTFPDTSTWPVVFPELPAAPYGGPCRPPQHTSKAAAP
CCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCC
RIPADRLPNHVAIVMDGNGRWATQRGLARTEGHKMGEAVVIDIACGAIELGIKWLSLYAF
CCCHHHCCCEEEEEECCCCCCHHHCCCHHHCCCCCCCEEEEEEECCHHHHHHHHHHHEEE
STENWKRSPEEVRFLMGFNRDVVRRRRDTLKKLGVRIRWVGSRPRLWRSVINELAVAEEM
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
TKSNDVITINYCVNYGGRTEITEATREIAREVAAGRLNPERITESTIARHLQRPDIPDVD
HCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCE
LFLRTSGEQRSSNFMLWQAAYAEYIFQDKLWPDYDRRDLWAACEEYASRTRRFGSA
EEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036