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The map label for this gene is glnA2

Identifier: 148662040

GI number: 148662040

Start: 2502304

End: 2503644

Strand: Reverse

Name: glnA2

Synonym: MRA_2239

Alternate gene names: 148662040

Gene position: 2503644-2502304 (Counterclockwise)

Preceding gene: 148662041

Following gene: 148662039

Centisome position: 56.64

GC content: 63.83

Gene sequence:

>1341_bases
ATGGACCGACAGAAGGAATTCGTTCTTCGTACCCTGGAAGAACGCGACATCCGCTTCGTCCGGCTGTGGTTCACAGACGT
GCTCGGTTTCCTCAAGTCGGTCGCCATCGCCCCAGCCGAACTCGAGGGCGCCTTCGAGGAAGGCATCGGCTTCGACGGAT
CCTCGATCGAGGGCTTTGCGCGGGTCTCGGAATCCGATACGGTGGCGCACCCGGACCCGTCGACCTTCCAGGTGCTGCCC
TGGGCCACCAGTTCCGGCCACCACCACTCAGCGCGGATGTTTTGCGACATCACCATGCCGGACGGCTCGCCGTCGTGGGC
GGACCCGCGGCACGTGTTGCGGCGGCAGCTGACGAAGGCCGGCGAACTCGGCTTCTCCTGCTACGTGCATCCCGAAATCG
AGTTCTTCCTGCTCAAGCCCGGACCCGAGGACGGGTCGGTGCCCGTCCCGGTCGACAACGCCGGCTATTTCGACCAAGCG
GTGCACGACTCCGCCTTGAACTTTCGCCGCCACGCGATCGATGCCCTGGAATTCATGGGCATCTCGGTGGAGTTCAGCCA
TCACGAAGGCGCACCCGGCCAGCAGGAGATCGACCTGCGGTTTGCCGACGCTCTGTCGATGGCTGACAACGTGATGACCT
TCCGCTACGTCATCAAAGAAGTCGCGCTGGAAGAGGGCGCCCGGGCGTCGTTCATGCCCAAGCCATTCGGCCAGCACCCG
GGCTCGGCGATGCACACCCACATGAGCCTGTTCGAGGGTGATGTCAACGCGTTCCACAGCGCTGATGATCCGCTGCAGCT
GTCGGAAGTGGGTAAATCGTTCATCGCCGGGATCCTGGAGCACGCTTGCGAGATCAGCGCGGTCACAAATCAGTGGGTCA
ACTCTTACAAGCGGCTGGTGCAGGGCGGCGAAGCGCCCACGGCCGCGTCGTGGGGGGCCGCCAACCGATCCGCCCTAGTG
CGGGTGCCGATGTACACGCCGCACAAGACCTCGTCGCGGCGGGTCGAAGTACGCAGCCCTGATTCGGCGTGCAATCCCTA
TCTGACATTCGCCGTGCTGCTGGCCGCGGGATTGCGGGGTGTAGAGAAGGGTTACGTGCTGGGCCCGCAGGCCGAGGACA
ACGTATGGGACCTCACACCCGAGGAACGCCGAGCGATGGGGTACCGAGAATTGCCGTCCAGTTTGGATAGTGCGCTGCGC
GCCATGGAGGCCTCCGAACTCGTCGCGGAGGCCTTGGGGGAGCACGTTTTTGACTTTTTCTTGCGCAACAAGCGCACGGA
GTGGGCGAACTACCGCAGCCACGTCACGCCATACGAGCTGCGCACCTACCTGTCGCTGTAG

Upstream 100 bases:

>100_bases
CCCTGAACTCACCGCGCACTCCCCTTGCGGGCCGCCCCGCAACGCTTCTGGCCGGGCATCGGTAACACGGTTTTAACAGA
CAGTGCATACTGTTCGAGGT

Downstream 100 bases:

>100_bases
CCCAGATCTACCGGTTGATCCGACGCCGACGCGCTTTGCGCTACCGTCGTGGTCGTGACCAAACTCGCCACGCAGCGGCC
GAAGTTGCCCAGCGTTGGCC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase 2

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFARVSESDTVAHPDPSTFQVLP
WATSSGHHHSARMFCDITMPDGSPSWADPRHVLRRQLTKAGELGFSCYVHPEIEFFLLKPGPEDGSVPVPVDNAGYFDQA
VHDSALNFRRHAIDALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHP
GSAMHTHMSLFEGDVNAFHSADDPLQLSEVGKSFIAGILEHACEISAVTNQWVNSYKRLVQGGEAPTAASWGAANRSALV
RVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSSLDSALR
AMEASELVAEALGEHVFDFFLRNKRTEWANYRSHVTPYELRTYLSL

Sequences:

>Translated_446_residues
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFARVSESDTVAHPDPSTFQVLP
WATSSGHHHSARMFCDITMPDGSPSWADPRHVLRRQLTKAGELGFSCYVHPEIEFFLLKPGPEDGSVPVPVDNAGYFDQA
VHDSALNFRRHAIDALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHP
GSAMHTHMSLFEGDVNAFHSADDPLQLSEVGKSFIAGILEHACEISAVTNQWVNSYKRLVQGGEAPTAASWGAANRSALV
RVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSSLDSALR
AMEASELVAEALGEHVFDFFLRNKRTEWANYRSHVTPYELRTYLSL
>Mature_446_residues
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFARVSESDTVAHPDPSTFQVLP
WATSSGHHHSARMFCDITMPDGSPSWADPRHVLRRQLTKAGELGFSCYVHPEIEFFLLKPGPEDGSVPVPVDNAGYFDQA
VHDSALNFRRHAIDALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHP
GSAMHTHMSLFEGDVNAFHSADDPLQLSEVGKSFIAGILEHACEISAVTNQWVNSYKRLVQGGEAPTAASWGAANRSALV
RVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSSLDSALR
AMEASELVAEALGEHVFDFFLRNKRTEWANYRSHVTPYELRTYLSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=442, Percent_Identity=27.8280542986425, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1790301, Length=475, Percent_Identity=35.3684210526316, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI87081870, Length=399, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): GLNA2_MYCBO (P64246)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855895.1
- ProteinModelPortal:   P64246
- SMR:   P64246
- EnsemblBacteria:   EBMYCT00000016461
- GeneID:   1091371
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2246c
- GeneTree:   EBGT00050000016709
- HOGENOM:   HBG553755
- OMA:   AMFDGSS
- ProtClustDB:   CLSK791706
- BioCyc:   MBOV233413:MB2246C-MONOMER
- BRENDA:   6.3.1.2
- GO:   GO:0005737
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- Gene3D:   G3DSA:3.30.590.10
- TIGRFAMs:   TIGR00653

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N; SSF54368 Gln_synt_beta

EC number: =6.3.1.2

Molecular weight: Translated: 49608; Mature: 49608

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHH
RVSESDTVAHPDPSTFQVLPWATSSGHHHSARMFCDITMPDGSPSWADPRHVLRRQLTKA
HCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
GELGFSCYVHPEIEFFLLKPGPEDGSVPVPVDNAGYFDQAVHDSALNFRRHAIDALEFMG
HCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHP
EEEEECCCCCCCCCHHEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GSAMHTHMSLFEGDVNAFHSADDPLQLSEVGKSFIAGILEHACEISAVTNQWVNSYKRLV
CHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
VEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSSLDSALRAMEASELVAEALGEHVFDFF
CCCCCEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNKRTEWANYRSHVTPYELRTYLSL
HHCCCCHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHH
RVSESDTVAHPDPSTFQVLPWATSSGHHHSARMFCDITMPDGSPSWADPRHVLRRQLTKA
HCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
GELGFSCYVHPEIEFFLLKPGPEDGSVPVPVDNAGYFDQAVHDSALNFRRHAIDALEFMG
HCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVALEEGARASFMPKPFGQHP
EEEEECCCCCCCCCHHEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GSAMHTHMSLFEGDVNAFHSADDPLQLSEVGKSFIAGILEHACEISAVTNQWVNSYKRLV
CHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
VEKGYVLGPQAEDNVWDLTPEERRAMGYRELPSSLDSALRAMEASELVAEALGEHVFDFF
CCCCCEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNKRTEWANYRSHVTPYELRTYLSL
HHCCCCHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972