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The map label for this gene is cobL

Identifier: 148661886

GI number: 148661886

Start: 2338874

End: 2340046

Strand: Reverse

Name: cobL

Synonym: MRA_2086

Alternate gene names: 148661886

Gene position: 2340046-2338874 (Counterclockwise)

Preceding gene: 148661887

Following gene: 148661885

Centisome position: 52.94

GC content: 68.2

Gene sequence:

>1173_bases
ATGATCATCGTTGTCGGGATCGGCGCCGACGGCATGACCGGTCTCTCCGAGCATTCTCGCTCCGAATTGCGCAGGGCCAC
AGTAATTTACGGCTCGAAACGGCAACTTGCCCTGCTCGACGATACCGTCACCGCCGAGCGCTGGGAGTGGCCGACGCCGA
TGCTGCCCGCGGTGCAAGGCCTGTCACCGGATGGGGCTGACCTACACGTGGTTGCCAGCGGCGACCCGTTGTTGCATGGT
ATCGGCTCCACCCTGATCCGGCTGTTCGGCCACGACAACGTGACCGTGTTGCCGCACGTGTCCGCGGTGACGTTGGCGTG
CGCCCGGATGGGCTGGAACGTGTATGACACCGAGGTGATCAGCCTGGTCACCGCGCAACCACACACCGCGGTGCGCCGCG
GCGGCCGGGCCATCGTGCTGTCCGGCGATCGGTCCACCCCGCAGGCGCTGGCGGTGCTGCTGACCGAGCACGGTCGCGGT
GACTCCAAGTTCAGCGTGCTCGAACAGCTTGGCGGCCCGGCCGAACGCCGCCGCGACGGTACGGCCCGGGCATGGGCCTG
CGACCCACCCCTCGATGTCGATGAGCTCAACGTGATCGCCGTGCGCTACCTGCTCGACGAGCGCACGTCGTGGGCACCCG
ACGAGGCATTCGCGCACGACGGGCAGATCACCAAACACCCGATCCGCGTGCTGACCCTGGCTGCGCTGGCGCCAAGGCCC
GGGCAGCGGTTATGGGACGTCGGCGCGGGCTCAGGCGCCATCGCGGTCCAGTGGTGTCGGAGCTGGCCGGGCTGCACCGC
GGTGGCGTTCGAGCGCGACGAACGGCGCCGCCGCAACATTGGGTTCAATGCCGCGGCCTTCGGGGTGAGCGTCGACGTGC
GCGGCGACGCGCCCGATGCGTTCGACGACGCCGCACGGCCGTCGGTGATTTTTCTTGGCGGTGGTGTAACCCAGCCAGGC
CTGCTTGAGGCCTGCCTGGACAGCCTGCCCGCAGGCGGGAACTTGGTCGCCAACGCTGTCACCGTCGAATCGGAAGCCGC
TCTGGCGCATGCATATTCGCGCCTCGGTGGCGAGCTACGACGATTCCAGCACTATCTCGGCGAACCGCTGGGCGGCTTCA
CCGGTTGGCGCCCACAGCTGCCGGTCACCCAGTGGTCGGTGACCAAGCGATGA

Upstream 100 bases:

>100_bases
CCCCGGTTCGTCTGGCGCAGGATGCCGCGATGATCCGCGACGATGCAGAGCGCAGCGATGCGGTGGGGGTACCGCCCGCT
TGCGGGGGAGAGCGGCGCCG

Downstream 100 bases:

>100_bases
CGGTCTATTTCATCGGAGCGGGCCCCGGCGCCGCCGACCTAATCACCGTCCGCGGCCAACGGCTCCTGCAACGATGCCCG
GTGTGCCTGTATGCGGGTTC

Product: precorrin-6y c5,15-methyltransferase

Products: NA

Alternate protein names: Precorrin-6 methyltransferase; Precorrin-6Y methylase

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MIIVVGIGADGMTGLSEHSRSELRRATVIYGSKRQLALLDDTVTAERWEWPTPMLPAVQGLSPDGADLHVVASGDPLLHG
IGSTLIRLFGHDNVTVLPHVSAVTLACARMGWNVYDTEVISLVTAQPHTAVRRGGRAIVLSGDRSTPQALAVLLTEHGRG
DSKFSVLEQLGGPAERRRDGTARAWACDPPLDVDELNVIAVRYLLDERTSWAPDEAFAHDGQITKHPIRVLTLAALAPRP
GQRLWDVGAGSGAIAVQWCRSWPGCTAVAFERDERRRRNIGFNAAAFGVSVDVRGDAPDAFDDAARPSVIFLGGGVTQPG
LLEACLDSLPAGGNLVANAVTVESEAALAHAYSRLGGELRRFQHYLGEPLGGFTGWRPQLPVTQWSVTKR

Sequences:

>Translated_390_residues
MIIVVGIGADGMTGLSEHSRSELRRATVIYGSKRQLALLDDTVTAERWEWPTPMLPAVQGLSPDGADLHVVASGDPLLHG
IGSTLIRLFGHDNVTVLPHVSAVTLACARMGWNVYDTEVISLVTAQPHTAVRRGGRAIVLSGDRSTPQALAVLLTEHGRG
DSKFSVLEQLGGPAERRRDGTARAWACDPPLDVDELNVIAVRYLLDERTSWAPDEAFAHDGQITKHPIRVLTLAALAPRP
GQRLWDVGAGSGAIAVQWCRSWPGCTAVAFERDERRRRNIGFNAAAFGVSVDVRGDAPDAFDDAARPSVIFLGGGVTQPG
LLEACLDSLPAGGNLVANAVTVESEAALAHAYSRLGGELRRFQHYLGEPLGGFTGWRPQLPVTQWSVTKR
>Mature_390_residues
MIIVVGIGADGMTGLSEHSRSELRRATVIYGSKRQLALLDDTVTAERWEWPTPMLPAVQGLSPDGADLHVVASGDPLLHG
IGSTLIRLFGHDNVTVLPHVSAVTLACARMGWNVYDTEVISLVTAQPHTAVRRGGRAIVLSGDRSTPQALAVLLTEHGRG
DSKFSVLEQLGGPAERRRDGTARAWACDPPLDVDELNVIAVRYLLDERTSWAPDEAFAHDGQITKHPIRVLTLAALAPRP
GQRLWDVGAGSGAIAVQWCRSWPGCTAVAFERDERRRRNIGFNAAAFGVSVDVRGDAPDAFDDAARPSVIFLGGGVTQPG
LLEACLDSLPAGGNLVANAVTVESEAALAHAYSRLGGELRRFQHYLGEPLGGFTGWRPQLPVTQWSVTKR

Specific function: Catalyzes the methylation of both C-5 and C-15 in precorrin-6Y to form precorrin-8X

COG id: COG2241

COG function: function code H; Precorrin-6B methylase 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBL_MYCTU (Q10671)

Other databases:

- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   C70765
- RefSeq:   NP_216588.1
- RefSeq:   NP_336598.1
- ProteinModelPortal:   Q10671
- EnsemblBacteria:   EBMYCT00000003371
- EnsemblBacteria:   EBMYCT00000073096
- GeneID:   888321
- GeneID:   923637
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2132
- KEGG:   mtu:Rv2072c
- TIGR:   MT2132
- TubercuList:   Rv2072c
- GeneTree:   EBGT00050000017618
- HOGENOM:   HBG537530
- OMA:   WRPAMPV
- ProtClustDB:   CLSK791605
- BRENDA:   2.1.1.132
- InterPro:   IPR000878
- InterPro:   IPR012818
- InterPro:   IPR014008
- InterPro:   IPR006365
- PIRSF:   PIRSF036428
- TIGRFAMs:   TIGR02467
- TIGRFAMs:   TIGR02469

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.132

Molecular weight: Translated: 41855; Mature: 41855

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVVGIGADGMTGLSEHSRSELRRATVIYGSKRQLALLDDTVTAERWEWPTPMLPAVQG
CEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHCC
LSPDGADLHVVASGDPLLHGIGSTLIRLFGHDNVTVLPHVSAVTLACARMGWNVYDTEVI
CCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCEECCEEE
SLVTAQPHTAVRRGGRAIVLSGDRSTPQALAVLLTEHGRGDSKFSVLEQLGGPAERRRDG
EEEECCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHCCC
TARAWACDPPLDVDELNVIAVRYLLDERTSWAPDEAFAHDGQITKHPIRVLTLAALAPRP
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCC
GQRLWDVGAGSGAIAVQWCRSWPGCTAVAFERDERRRRNIGFNAAAFGVSVDVRGDAPDA
CCCEEECCCCCCCEEEEEHHCCCCCEEEEECCHHHHHHCCCCCEEEEEEEEEECCCCCCC
FDDAARPSVIFLGGGVTQPGLLEACLDSLPAGGNLVANAVTVESEAALAHAYSRLGGELR
HHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHH
RFQHYLGEPLGGFTGWRPQLPVTQWSVTKR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIIVVGIGADGMTGLSEHSRSELRRATVIYGSKRQLALLDDTVTAERWEWPTPMLPAVQG
CEEEEEECCCCCCCCHHHHHHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHCC
LSPDGADLHVVASGDPLLHGIGSTLIRLFGHDNVTVLPHVSAVTLACARMGWNVYDTEVI
CCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCEECCEEE
SLVTAQPHTAVRRGGRAIVLSGDRSTPQALAVLLTEHGRGDSKFSVLEQLGGPAERRRDG
EEEECCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHCCC
TARAWACDPPLDVDELNVIAVRYLLDERTSWAPDEAFAHDGQITKHPIRVLTLAALAPRP
CCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCC
GQRLWDVGAGSGAIAVQWCRSWPGCTAVAFERDERRRRNIGFNAAAFGVSVDVRGDAPDA
CCCEEECCCCCCCEEEEEHHCCCCCEEEEECCHHHHHHCCCCCEEEEEEEEEECCCCCCC
FDDAARPSVIFLGGGVTQPGLLEACLDSLPAGGNLVANAVTVESEAALAHAYSRLGGELR
HHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHH
RFQHYLGEPLGGFTGWRPQLPVTQWSVTKR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036