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The map label for this gene is iciA [C]

Identifier: 148661796

GI number: 148661796

Start: 2238891

End: 2239802

Strand: Reverse

Name: iciA [C]

Synonym: MRA_1998

Alternate gene names: 148661796

Gene position: 2239802-2238891 (Counterclockwise)

Preceding gene: 148661801

Following gene: 148661794

Centisome position: 50.67

GC content: 65.02

Gene sequence:

>912_bases
ATGGTGGATCCGCAGCTTGACGGTCCACAGCTGGCCGCATTGGCTGCCGTGGTCGAACTGGGCAGCTTCGATGCGGCCGC
GGAGCGCCTACATGTCACCCCGTCGGCTGTCAGTCAGCGCATCAAGTCGTTGGAGCAGCAGGTCGGCCAGGTGCTGGTGG
TCAGGGAAAAGCCATGTCGGGCGACGACCGCAGGTATCCCGCTGTTGCGGTTGGCCGCGCAAACAGCGTTGCTCGAGTCC
GAGGCGCTCGCTGAAATGGGTGGCAACGCGTCGCTGAAACGCACGCGGATCACCATTGCGGTAAACGCCGATTCCATGGC
GACATGGTTTTCGGCCGTGTTCGACGGTCTCGGCGACGTCCTGCTCGACGTTCGGATCGAGGACCAGGACCATTCCGCGC
GGCTGCTACGGGAGGGTGTGGCGATGGGCGCGGTGACCACCGAGCGGAACCCGGTGCCGGGCTGCCGGGTGCACCCGCTG
GGTGAAATGCGCTACCTACCAGTGGCCAGCAGGCCATTCGTCCAGCGCCATCTATCCGACGGGTTCACTGCCGCCGCGGC
GGCTAAAGCTCCGTCACTGGCGTGGAATCGTGACGATGGGCTGCAGGACATGTTGGTGCGTAAGGCCTTTCGTCGCGCCA
TCACCAGACCGACGCACTTTGTCCCGACCACAGAGGGCTTCACCGCCGCAGCGCGCGCCGGGCTGGGATGGGGCATGTTC
CCCGAGAAGCTGGCAGCATCTCCGCTTGCCGATGGATCGTTCGTACGGGTCTGCGACATACACCTCGACGTCCCTCTCTA
TTGGCAATGCTGGAAACTGGACAGTCCGATCATCGCGCGAATTACCGACACGGTGAGGGCGGCGGCAAGCGGTCTGTACC
GGGGCCAGCAACGCCGCCGCCGACCGGGTTGA

Upstream 100 bases:

>100_bases
CACTAGGCAGTTTGTATGGGTCAGTATAGCTAATAATTCTTCATTTACATTAGCATTATTAATGTGCAGTGCGACGCTCC
GCAGATGGTCTACACCTGAG

Downstream 100 bases:

>100_bases
CCGACGCCAGCATGTTGTTGTGTTAGCGCGGCTTGGTCTCGATGTCCCGGCCTTGCTGGACCCGCTTCCTCAAACAGGTT
GAACTTAACGACTCAGACGG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MVDPQLDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVREKPCRATTAGIPLLRLAAQTALLES
EALAEMGGNASLKRTRITIAVNADSMATWFSAVFDGLGDVLLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPL
GEMRYLPVASRPFVQRHLSDGFTAAAAAKAPSLAWNRDDGLQDMLVRKAFRRAITRPTHFVPTTEGFTAAARAGLGWGMF
PEKLAASPLADGSFVRVCDIHLDVPLYWQCWKLDSPIIARITDTVRAAASGLYRGQQRRRRPG

Sequences:

>Translated_303_residues
MVDPQLDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVREKPCRATTAGIPLLRLAAQTALLES
EALAEMGGNASLKRTRITIAVNADSMATWFSAVFDGLGDVLLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPL
GEMRYLPVASRPFVQRHLSDGFTAAAAAKAPSLAWNRDDGLQDMLVRKAFRRAITRPTHFVPTTEGFTAAARAGLGWGMF
PEKLAASPLADGSFVRVCDIHLDVPLYWQCWKLDSPIIARITDTVRAAASGLYRGQQRRRRPG
>Mature_303_residues
MVDPQLDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVREKPCRATTAGIPLLRLAAQTALLES
EALAEMGGNASLKRTRITIAVNADSMATWFSAVFDGLGDVLLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPL
GEMRYLPVASRPFVQRHLSDGFTAAAAAKAPSLAWNRDDGLQDMLVRKAFRRAITRPTHFVPTTEGFTAAARAGLGWGMF
PEKLAASPLADGSFVRVCDIHLDVPLYWQCWKLDSPIIARITDTVRAAASGLYRGQQRRRRPG

Specific function: Specific Inhibitor Of Chromosomal Initiation Of Replication In VItro. Binds The Three 13-Mers In The Origin (Oric) To Block Initiation Of Replication. [C]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789283, Length=281, Percent_Identity=35.9430604982206, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1985_MYCTU (P67665)

Other databases:

- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   G70756
- RefSeq:   NP_216501.1
- RefSeq:   NP_336500.1
- PDB:   3ISP
- PDBsum:   3ISP
- ProteinModelPortal:   P67665
- EnsemblBacteria:   EBMYCT00000001062
- EnsemblBacteria:   EBMYCT00000071151
- GeneID:   885818
- GeneID:   923399
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2039
- KEGG:   mtu:Rv1985c
- TIGR:   MT2039
- TubercuList:   Rv1985c
- GeneTree:   EBGT00050000015807
- HOGENOM:   HBG685425
- OMA:   HCWNLES
- ProtClustDB:   PRK03635
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039
- TIGRFAMs:   TIGR03298

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 32837; Mature: 32837

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDPQLDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVREKPCR
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCHHHHHHHHHHHHHHHCCEEEEECCCCC
ATTAGIPLLRLAAQTALLESEALAEMGGNASLKRTRITIAVNADSMATWFSAVFDGLGDV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHE
LLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPLGEMRYLPVASRPFVQRHLSD
EEEEEECCCCHHHHHHHHHHHHCEEECCCCCCCCCEECCCCCEEECCCCCCHHHHHHHCC
GFTAAAAAKAPSLAWNRDDGLQDMLVRKAFRRAITRPTHFVPTTEGFTAAARAGLGWGMF
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCC
PEKLAASPLADGSFVRVCDIHLDVPLYWQCWKLDSPIIARITDTVRAAASGLYRGQQRRR
HHHHHCCCCCCCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
RPG
CCC
>Mature Secondary Structure
MVDPQLDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVREKPCR
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCHHHHHHHHHHHHHHHCCEEEEECCCCC
ATTAGIPLLRLAAQTALLESEALAEMGGNASLKRTRITIAVNADSMATWFSAVFDGLGDV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHE
LLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPLGEMRYLPVASRPFVQRHLSD
EEEEEECCCCHHHHHHHHHHHHCEEECCCCCCCCCEECCCCCEEECCCCCCHHHHHHHCC
GFTAAAAAKAPSLAWNRDDGLQDMLVRKAFRRAITRPTHFVPTTEGFTAAARAGLGWGMF
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCC
PEKLAASPLADGSFVRVCDIHLDVPLYWQCWKLDSPIIARITDTVRAAASGLYRGQQRRR
HHHHHCCCCCCCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
RPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036