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The map label for this gene is hcaD [C]

Identifier: 148661618

GI number: 148661618

Start: 2064089

End: 2065291

Strand: Reverse

Name: hcaD [C]

Synonym: MRA_1824

Alternate gene names: 148661618

Gene position: 2065291-2064089 (Counterclockwise)

Preceding gene: 148661619

Following gene: 148661611

Centisome position: 46.73

GC content: 66.75

Gene sequence:

>1203_bases
TTGACCCGGGTGGTGGTGATCGGCTCCGGGTTCGCGGGCCTGTGGGCGGCGCTCGGTGCGGCCCGACGACTCGACGAGCT
CGCCGTCCTAGCGGGCACGGTCGACGTCATGGTGGTGAGCAACAAACCGTTTCATGATATCCGGGTTCGCAACTATGAGG
CTGATCTGAGCGCCTGCCGCATCCCGCTCGGCGACGTGCTCGGCCCTGCCGGCGTTGCGCATGTCACCGCCGAGGTGACC
GCGATCGACGCCGACGGCCGCCGCGTCACCACGTCGACCGGTGCCTCATATAGCTATGACCGACTGGTCCTCGCGTCGGG
CAGTCACGTGGTCAAACCCGCGTTGCCAGGACTGGCAGAGTTCGGTTTTGATGTCGACACGTACGACGGCGCGGTCAGGC
TACAGCAGCATCTGCAAGGACTGGCCGGCGGGCCGCTGACATCGGCGGCGGCAACCGTCGTGGTCGTCGGTGCCGGGCTG
ACCGGTATCGAGACGGCCTGCGAGTTGCCGGGCCGACTGCACGCGTTGTTCGCCCGTGGTGATGGTGTCACCCCCCGCGT
GGTACTCATCGACCACAACCCCTTCGTCGGCTCCGATATGGGTTTATCGGCGCGGCCGGTGATCGAACAGGCGCTGTTGG
ACAACGGCGTCGAGACCAGAACCGGCGTGAGCGTTGCCGCCGTCAGCCCGGGCGGTGTAACGCTGTCATCGGGGGAACGG
CTGGCAGCGGCCACCGTGGTGTGGTGCGCCGGCATGCGGGCCAGCCGCCTGACCGAGCAGCTGCCGGTAGCCCGTGATCG
GCTGGGACGCCTGCAAGTCGACGACTACCTGCGGGTGATCGGGGTGCCTGCGATGTTTGCTGCTGGCGATGTGGCCGCGG
CCCGGATGGATGACGAGCACCTGTCGGTCATGTCGTGTCAGCATGGGCGCCCGATGGGCCGCTATGCCGGCTGCAACGTC
ATCAACGATCTGTTCGACCAACCGTTGCTGGCTCTTCGGATCCCTTGGTATGTCACGGTTCTCGATCTAGGATCGGCGGG
CGCGGTGTACACCGAGGGATGGGAACGCAAAGTGGTATCGCAGGGCGCACCGGCCAAGACCACCAAGCAGTCAATCAATA
CCCGGCGGATCTACCCGCCGCTGAACGGCAGTCGGGCAGACTTGCTGGCTGCGGCCGCGCCGCGAGTACAGCCCCGCCCC
TAG

Upstream 100 bases:

>100_bases
TCGGATGGCCCGGGCTCGAAGGAGGTTGGCACGTGTGGATGTTCCTGCCCGGTCGCCACGCGGCGGCGGCGATACCCCAG
AGTCGTCAGGTGTTTAGCCG

Downstream 100 bases:

>100_bases
TGTCGTGGGTCATTCGGCCTGCGCGTGCTCACCGGCATACCAGTGCACAGCGTAAACGCCCGGCTGCAGCGACAGTTCCG
CCACCAACCGCTCTAGCTTG

Product: putative dehydrogenase

Products: NAD+; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTRVVVIGSGFAGLWAALGAARRLDELAVLAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPLGDVLGPAGVAHVTAEVT
AIDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGL
TGIETACELPGRLHALFARGDGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGER
LAAATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSVMSCQHGRPMGRYAGCNV
INDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP

Sequences:

>Translated_400_residues
MTRVVVIGSGFAGLWAALGAARRLDELAVLAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPLGDVLGPAGVAHVTAEVT
AIDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGL
TGIETACELPGRLHALFARGDGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGER
LAAATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSVMSCQHGRPMGRYAGCNV
INDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP
>Mature_399_residues
TRVVVIGSGFAGLWAALGAARRLDELAVLAGTVDVMVVSNKPFHDIRVRNYEADLSACRIPLGDVLGPAGVAHVTAEVTA
IDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAEFGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLT
GIETACELPGRLHALFARGDGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGERL
AAATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEHLSVMSCQHGRPMGRYAGCNVI
NDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVSQGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP

Specific function: Part Of Multicomponent 3-Phenylpropionate Dioxygenase. That Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG1252

COG function: function code C; NADH dehydrogenase, FAD-containing subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH dehydrogenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1812_MYCTU (O07220)

Other databases:

- EMBL:   BX842577
- EMBL:   AE000516
- PIR:   F70982
- RefSeq:   NP_216328.1
- RefSeq:   NP_336319.1
- ProteinModelPortal:   O07220
- EnsemblBacteria:   EBMYCT00000001140
- EnsemblBacteria:   EBMYCT00000070469
- GeneID:   885487
- GeneID:   923775
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1860
- KEGG:   mtu:Rv1812c
- TIGR:   MT1860
- TubercuList:   Rv1812c
- GeneTree:   EBGT00050000014578
- HOGENOM:   HBG289292
- OMA:   ACELPAR
- ProtClustDB:   CLSK867445
- InterPro:   IPR013027
- InterPro:   IPR001327
- InterPro:   IPR000103
- PRINTS:   PR00368
- PRINTS:   PR00469

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2

EC number: 1.6.99.3

Molecular weight: Translated: 41957; Mature: 41825

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVVVIGSGFAGLWAALGAARRLDELAVLAGTVDVMVVSNKPFHDIRVRNYEADLSACR
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECEECCHHHHC
IPLGDVLGPAGVAHVTAEVTAIDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAE
CCHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCEEEEECCCEEECCCCCCHHH
FGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGIETACELPGRLHALFARG
HCCCCCCCCHHHHHHHHHHHCCCCCCHHHCEEEEEEECCCCCHHHHHCCCCCEEEEEECC
DGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGER
CCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCC
LAAATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEH
HHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCHHHHCCCHHHHHCCCHH
LSVMSCQHGRPMGRYAGCNVINDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVS
HHEEECCCCCCCCHHHCHHHHHHHHCCCEEEEECCEEEEEEECCCCCCEECCCHHHHHHH
QGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP
CCCCCCHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TRVVVIGSGFAGLWAALGAARRLDELAVLAGTVDVMVVSNKPFHDIRVRNYEADLSACR
CEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECEECCHHHHC
IPLGDVLGPAGVAHVTAEVTAIDADGRRVTTSTGASYSYDRLVLASGSHVVKPALPGLAE
CCHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCCEEEEECCCEEECCCCCCHHH
FGFDVDTYDGAVRLQQHLQGLAGGPLTSAAATVVVVGAGLTGIETACELPGRLHALFARG
HCCCCCCCCHHHHHHHHHHHCCCCCCHHHCEEEEEEECCCCCHHHHHCCCCCEEEEEECC
DGVTPRVVLIDHNPFVGSDMGLSARPVIEQALLDNGVETRTGVSVAAVSPGGVTLSSGER
CCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCC
LAAATVVWCAGMRASRLTEQLPVARDRLGRLQVDDYLRVIGVPAMFAAGDVAAARMDDEH
HHHHHHHHHCCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCHHHHCCCHHHHHCCCHH
LSVMSCQHGRPMGRYAGCNVINDLFDQPLLALRIPWYVTVLDLGSAGAVYTEGWERKVVS
HHEEECCCCCCCCHHHCHHHHHHHHCCCEEEEECCEEEEEEECCCCCCEECCCHHHHHHH
QGAPAKTTKQSINTRRIYPPLNGSRADLLAAAAPRVQPRP
CCCCCCHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; acceptor

Specific reaction: NADH + H+ + acceptor = NAD+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036